초록 ▼

1. 국내 식물 바이오매스의 현황조사, 미국재생에너지연구소의 표준분석법(www.eere.energy./biomass/analytical_procedure. html) 및 참고문헌조사에 따라 HPLC, GC, 회화로등의 분석장비를 이용한 바이오매스 시료의 표준물질 분석
- 셀룰로오스, 헤미셀룰로오스, 리그닌등의 함량분석
- BSA(bicinchoninic acid)법 및 흡광광도법을 이용한 단백질 정량분석, 기타 유기산, 당, Cell-bound-MnP, Intact-cell-bound-MnP, 그밖의 extractive등

1. 국내 식물 바이오매스의 현황조사, 미국재생에너지연구소의 표준분석법(www.eere.energy./biomass/analytical_procedure. html) 및 참고문헌조사에 따라 HPLC, GC, 회화로등의 분석장비를 이용한 바이오매스 시료의 표준물질 분석
- 셀룰로오스, 헤미셀룰로오스, 리그닌등의 함량분석
- BSA(bicinchoninic acid)법 및 흡광광도법을 이용한 단백질 정량분석, 기타 유기산, 당, Cell-bound-MnP, Intact-cell-bound-MnP, 그밖의 extractive등의 정량 및 정성적 분석
2. RSM을 이용하여 효소적 당화율을 높이기 위한 최적 전처리 조건 확립
- 전자빔 전처리: 1 MeV, 80 KGy 및 0.12mA의 변화에 따른 조건 최적화로 약 55% 가량의 효소적 당화율
-암모니아 전처리: 온도(50-${70^{\circ}C}$), 시간(4~12시간), 암모니아 농도(12~28%) 그리고 solid/liquid 비율(1:6)의 변화에 따른 조건 최적화로 70% 이상의 효소적 당화율
- 생물학적 전처리: RSM 을 활용한 합성배지의 조성변화에 의한 백색부후균의 리그닌 분해효소의 활성 최적화로 68% 가량의 효소적 당화율
: 변이에 의한 효소적 당화율 개선
3. 신개념의 물리적 및 생물학적 통합 바이오매스 전처리공정의 개발
- 최적조건에서의 전자빔처리를 기초로한 암모니아 및 생물학적 전처리의 병행실시 하였을 때 약 1.5 배 이상의 효소적 당화율 상승
4. 바이오매스의 전처리 조건변화에 따른 효소적 당화율 비교 및 효과확인
-전처리 단계별 XRD 분석 및 SEM 확인
5. 바이오에탄올 생산
- 통합적 전처리의 최적화와 1.1-1.8 배 가량의 수율 향상

Abstract ▼

The hydrolysis and fermentation of rice straw was used the combined pretreatment using all electron beam, ammonia and fungi at optimized condition. First of all, the pretreatment by electron beam irradiation (EBI) was found to significantly increase the enzyme digestibility of rice straw. Specifical

The hydrolysis and fermentation of rice straw was used the combined pretreatment using all electron beam, ammonia and fungi at optimized condition. First of all, the pretreatment by electron beam irradiation (EBI) was found to significantly increase the enzyme digestibility of rice straw. Specifically, when rice straw that was pretreated by EBI at 80 kGy at 0.12 mA and 1 MeV was hydrolyzed with 60 FPU of cellulase and 30 CBU of b-glucosidase, the glucose yield after 132 h of hydrolysis was 52.1% of theoretical maximum. This value was significantly higher than the 22.6% that was obtained when untreated rice straw was used. In addition, SEM analysis of pretreated rice straw revealed that EBI caused apparent damage to the surface of the rice straw. Furthermore, EBI pretreatment was found to increase the crystalline portion of the rice straw. Finally, the crystallinity and enzyme digestibility were found to be strongly correlated between rice straw samples that were pretreated by EBI under different conditions. Second, the ranges of the batch reaction condition stested were a temperature of 50-${70^{\circ}C}$, are action time of 4-12h, and an ammonia concentration of 12-28%(w/w) at a fixed S:L ratio of 1:6. The experimental and RSM results showed that the effects of reaction time and temperature were significant on the degree of lignin removal and enzymatic digestibility of the pretreated rice straw The optimal reaction condition for achieving the maximal enzymatic digestibility, 71.1%, was found to be ${69^{\circ}C}$, 10 h, 21% (w/w) of ammonia concentration and S:L ratio of 1:6. Different loading amounts of different commercial cellulases and the additional effects of a non-cellutolytic enzyme, xylanase, were also tested. Scanning electron micrographs showed that the specific surface area of cellulose available for the adsorption of cellulases possibly increased after the ammonia pretreatment. The simultaneous saccharification and fermentation (SSF) process was conducted to assess the ethanol production yield and productivity based on 3% (w/v) of the initial glucan concentration at ${38^{\circ}C}$ and 150 rpm. Third, the production of a fungal lignin-degrading enzyme, manganese peroxidase (MnP) was optimized by white rot fungi. Various culture conditions such as carbon, nitrogen, trace minerals, and vitamin sources were optimized against the activity of MnP by the response surface methodology (RSM). For the direct measurement of change in the enzyme digestibility by using cellulose hydrolysis enzymes (Trichoderma reesei cellulase and Asrersillus niger beta-glucosidase) of biomass in the fungal culture media, the rice straw samples were periodically taken from the culture media, and the enzyme digestibility of the cellulose in the rice straw was measured. The typical enzyme digestibility of rice straw was 22.6 and 69.4% for untreated and pretreated biomass, respectively, in terms of % of theoretical maximum of gluose hydrolysis. The overall mass balance of combined pretreatinent including the enzymatic digestibility and the ethanol production by using SSF was also determined.

목차 Contents

• 표지 ...1
• 제출문...2
• 보고서 초록...3
• 요약문...4
• SUMMARY...22
• 목차...24
• 제 1 장 연구개발과제의 개요 ...25
• ▶ 연구개발 개요...25
• ▶ 개요...27
• ▶ 바이오매스 처리공정...27
• ▶ 전처리후 바이오에탄올 생산성 향상...28
• ▶ 국내외 바이오매스자원의 현황 및 이용활성화 방안...29
• ▶ 개요...30
• 제 2 장 국내외 기술개발 현황 ...31
• 가. 유사 연구사례에 대한 조사 현황...31
• 제 3 장 연구개발수행 내용 및 결과 ...39
• 연구개발 추진계획...39
• 제 4 장 목표달성도 및 관련분야에의 기여도 ...49
• 1. 연구개발결과의 우수성 및 창의성...49
• 2. 연구개발결과의 파급효과...49
• 3. 연구개발결과에 대한 활용가능성...50
• 4. 연구개발 수행노력의 성실도...50
• 5. 공개발표된 연구개발성과(논문.지적재산권.발표회 개최 등)...50
• 제 5 장 연구개발결과의 활용계획 ...56
• * 추가연구의 필요성...56
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ...73
• # 에너지 기초.원천기술 분야 국제협력 추진 (과학기술부 에너지환경심의관실)...73
• 제 7 장 참고문헌 ...77
• 연구개발 요약서...82
• 연구결과활용계획서...84
• [첨부 1] 연구결과활용계획서...85
• [첨부 2] 기술 요약서...93
• 연구계획서 수정.보완요구사항 반영내역...98