본 사업과 관련하여 3단계 연구기간동안 SCI급 11 편, 학진등재 논문 5 편을 게재하였고 특허는 출원 9건, 등록 3건의 실적을 올렸음. 대표적인 논문으로 2010년 2월에 Metabolism이라는 잡지에 발효인삼의 주성분인 IH-901의 포도당 및 지질대사에 미치는 영향을 보고한 바 있음. 발효인삼의 경우 전임상, 인체시험을 완료하였음. 정상혈당 또는 공복혈당 장애가 있는 피험자 60명을 대상으로 혈당개선에 대한 발효인삼농축액 (960 mg/day)의 유효성 및 안전성 평가를 위한 8주간의 무작위, 이중눈가림, 플라시보 비교
본 사업과 관련하여 3단계 연구기간동안 SCI급 11 편, 학진등재 논문 5 편을 게재하였고 특허는 출원 9건, 등록 3건의 실적을 올렸음. 대표적인 논문으로 2010년 2월에 Metabolism이라는 잡지에 발효인삼의 주성분인 IH-901의 포도당 및 지질대사에 미치는 영향을 보고한 바 있음. 발효인삼의 경우 전임상, 인체시험을 완료하였음. 정상혈당 또는 공복혈당 장애가 있는 피험자 60명을 대상으로 혈당개선에 대한 발효인삼농축액 (960 mg/day)의 유효성 및 안전성 평가를 위한 8주간의 무작위, 이중눈가림, 플라시보 비교 인체실험결과 발효인삼농축액은 임상적으로 이상반응이나 변화를 일으키지 않아 안전성 측면에서 문제가 없을 것으로 판단되며 약효 면에서는 공복 및 식후 혈당을 유의하게 감소시켰음.
Abstract▼
This project was to design to develop a noble anti-diabetic agent from indigenous plants cultivated in Korea peninsula with better efficacy and tolerable unwanted side effects when compared to commercially available anti-diabetic agents. Among hundreds of indigenous plants, ginseng and c
This project was to design to develop a noble anti-diabetic agent from indigenous plants cultivated in Korea peninsula with better efficacy and tolerable unwanted side effects when compared to commercially available anti-diabetic agents. Among hundreds of indigenous plants, ginseng and cinnamon were shown to have superb anti-diabetic effects through in vitro and in vivo studies. Fermented ginseng, which was prepared by treating white ginseng extract with a beta-glucosidase, resulting in ginseng extract containing relatively large amounts of IH-901 (compound K). IH-901 is an intestinal metabolite of ginsenosides found in panax ginseng. In the present study, effects of CK on glucose and lipid metabolisms were examined using C2C12 myotubes and C57BL/ksJ db/dbmice. A significant increase in phosphorylated AMP-activated protein kinase (AMPK) was observed when differentiated C2C12 myotubes were treated with IH -901. Glucose tsensporter4 (GLUT4) protein expressions were also up-regulated when muscle cells were treated with of IH -901 up to 60 min, resulting in stimulation of glucose uptake by 25% as compared to untreated cells. In addition, phosphatidylinositol-3 kinase (PI3K) and Akt protein expressions were increased when C2C12 myotubes were exposed to IH -901 for up to 3 hour; and these effects including glucose uptake were attenuated by pretreatment with L Y29pr02, a selective PI3K inhibitor. In animal study, IH -901 at 25 mg/kg lowered the plasma glucose, triglyceride, cholesterol and non-esterified fatty acid levels by 20.7%, 41.6%, 20.0% and 24.6%, respectively, compared to control mice. In the meantime, plasma insulin levels were significantly increased by 2.2 and 3.4 times in 10 and 25 mg/kg -treated mice, respectively, compared to control mice, in parallel with the histological observation showing a preserved architecture of the pancreatic islet. Protein and gene expression patterns for AMPK, SREBPla and GLUT4 in the liver and skeletal muscles were similar to those in cell studies. We also investigated the protective effects of fermented ginseng (FG) on hyperglycemia induced by strepto2otocin (STZ) in Sprague Dawley rats. FG was administered orally at dose of 250 (FGL) or 500 mg/kg (FGH) for 20 days starting one week before STZ injection. FG restored the plasma insulin levels by 266% and 334% in FGL and FGH, respectively, and resulting in reduction of plasma glucose concentration. Histological observation indicated that STZ-induced destruction of pancreatic islets was protected by FG. In the random, double blind and placebo-controlled clinical study, only one person showed side effects and 59 persons were reported to have lowered fasting and postprandial plasma glucose levels when compared the control group. In summary, fermented ginseng might be a promising therapeutic agent improving altered glucose and lipid metabolisms revealed in type 2 diabetic patients.
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