초록 ▼

1. 갈매보리수나무 신품종 개발 및 대량증식 기술 개발
- 갈매보리수나무 국내 도입 및 삽목 증식법 확립, 기내대량증식 시스템 개발
2. 갈매보리수나무 기능성 화장품 개발
- 갈매보리수나무 기능성 물질 추출기술 개발, 효능평가
- 갈매보리수나무 종자 Ethanol fraction (SE)의 특성
- 종자 Ethanol fraction의 피부효능 평가, Ethanol 추출물의 항산화 및 항균 거동 조사
3. 갈매보리수나무를 활용한 고부가가치 기능성 식품 및 소재개발 연구
- 갈매보리수나무의 생리활

1. 갈매보리수나무 신품종 개발 및 대량증식 기술 개발
- 갈매보리수나무 국내 도입 및 삽목 증식법 확립, 기내대량증식 시스템 개발
2. 갈매보리수나무 기능성 화장품 개발
- 갈매보리수나무 기능성 물질 추출기술 개발, 효능평가
- 갈매보리수나무 종자 Ethanol fraction (SE)의 특성
- 종자 Ethanol fraction의 피부효능 평가, Ethanol 추출물의 항산화 및 항균 거동 조사
3. 갈매보리수나무를 활용한 고부가가치 기능성 식품 및 소재개발 연구
- 갈매보리수나무의 생리활성 물질 검정 연구
- 식품 소재로서의 기호성 검정 연구, 기능성 소재개발
4. 갈매보리수나무의 생리생태적 특성 연구 및 재배법 확립
- 갈매보리수나무 생태적지(자생지)의 환경특성 분석
- 갈매보리수나무의 질소고정 효과 연구
- 갈매보리수나무의 생리생태적 특성 및 재배법 규명

Abstract ▼

(1) Development of mass propagation technology in H. rhamnoides
(a) Introduction of Domestic and Establish of cutting system
◦ Growing of superior plant after Obtain clones and seed from desert, mainly in China and Mongolia
◦ Cutting wood and seedling from seed
◦ Screening for unique cha

(1) Development of mass propagation technology in H. rhamnoides
(a) Introduction of Domestic and Establish of cutting system
◦ Growing of superior plant after Obtain clones and seed from desert, mainly in China and Mongolia
◦ Cutting wood and seedling from seed
◦ Screening for unique characteristics
(b) Research of plant tissue culture in in vitro
◦ Material as Apical meristem for plantlet in in vitro
(c) Development of Mass propagation system through Somatic-embryo
◦ PGRs of auxin(2,4-D), cytokinin (BA, zeatin, TDZ), ABA, GA etc., treated for induced embryo from cotyledon and leafe
◦ Mass-plantlet apply to germination and ventilization technology from somatic-embryo
(2) Development of Fucntional cosmetics use H. rahmnoides
(a) Extraction of bioactives from H. rahmnoides
◦ solvent extraction: hexane, ethanol, methanol, hot water
◦ oil fractionation
(b) Bioactivities of the fractions from H. rahmnoides
◦ antioxidant effect
◦ antiaging effect
(c) Characteristics of the ethanol fraction from H. rahmnoides
◦ TLC
◦ HPLC
◦ ESI-MS
(d) Mass production of SE fraction
◦ hexane-ethanol process
◦ ethanol-hexane process
(e) Skin protecting effect of the Ethanol fraction from H. rahmnoides
◦ effect of the extraction process on the efficacy of SE fraction
◦ effect of SE fraction extracted with ethanol only
(f) The Antioxidant and antibacterial behavior examination for sediment removed ethanol extract from Sea buckthorn seeds
◦ development of sediment removing technology for ethanol extract from Sea buckthorn seeds
◦ evaluation of antioxidant efficacy for sediment removed ethanol extract from Sea buckthorn seeds
◦ evaluation of antibacterial efficacy for sediment removed ethanol extract from Sea buckthorn seeds
(g) Development of the prototype cosmetics
◦ prototype development of cosmetics adding sediment removed ethanol extract from Sea buckthorn seeds
(h) Potential of the leaf extract
(3) Development of a high value functional food and material by using Sea buckthorn
(a) Reserch for test bioacitve substance of Sea buckthorn
◦ Evaluation of soluble vitamin C content
◦ Evaluation chemical composition: Pretein, Carbohydrate, fat content
◦ For the possibility of development, evaluate the multi-functional effect including anticancer and anti-oxidant through in vitro assay : Establish the evaluation system and discover biological metabolism of unknown functional ingredient in H. rhamnoides L. through in vitro assay.
◦ Investigation of multi-functional effect and biological mechanism for suggesting biological basis : For the development of pharmaceutical technique and products, investigate biological metabolism related to anticancer and anti-oxidant activity.
◦ Natural anti-bacterial product application of H. rhamnoides L. fruit extracts : Anti-bacterial effect of H. rhamnoides L. fruit extracts
(b) Research preference test for food material
◦ Analysis of volatile compounds of Sea buckthorn fruit extract with Solid-phase micro extraction (SPME) and Solvent assisted flavor evaporation (SAFE) by Gas chromatography massspectrometry (GC-MS)
(c) Development of functional food
◦ Development a functional beer contained Sea buckthorn fruit extract by application beer brewing technology
◦ A study which develop a recipe for functional jellies that are made up of heating mixing process with gelatin, sugar, sea buckthorn berry juice, and cooling process.
◦ A study which develop a recipe for functional beverages that are made up of mixing process with sea buckthorn berry juice, sugar, citric acid, and other fruit ingredients for improving palatability.
◦ Evaluate multi-functional effect and suitability to human metabolism: For development of trial manufactured goods, conduct Proteomics targeted to human cell.
◦ Investigate biological metabolism and suitability to human : Evaluation of protein expression level reflects the suitability to human.
(4) Study on ecophysiological characteristics and cultivation method of Hippophae rhamnoides
(a) Analysis of habit environment characteristics of H. rhamnoides
◦ Investigation of habit climate characteristics of H. rhamnoides
(b) Analysis of nitrogen fixation effect in H. rhamnoides
◦ Investigation of soil properties such as nitrogen and organic matter contents before and after H. rhamnoides seedlings were planted
(c) Analysis of ecophysiological characteristics and cultivation method of H. rhamnoides
◦ Comparison of growth of seedlings by light conditions (quality and quantity)
◦ Survival and growth response of seeds and seedlings at different irrigation conditions
◦ Growth and chlorophyll contents of seedlings H. rhamnoides at different fertilization conditions
◦ Physiological characteristics of seeds with different ages
- Analysis of inorganic compounds and electrical conductivity of leachate
◦ Mass propagation by various pre-treatments of seeds
- prechilling, cold stratification and priming

목차 Contents

• 제 출 문 ... 1
• 보고서 요약서 ... 2
• 요 약 문 ... 3
• SUMMARY ... 9
• CONTENTS ... 17
• 목 차 ... 18
• 제 1 장. 연구개발과제의 개요 ... 19
• 제 1 절. 갈매보리수나무 대량증식 기술 개발 ... 19
• 제 2 절. 갈매보리수나무 기능성 화장품 ... 20
• 제 3 절. 갈매보리수나무를 활용한 고부가가치 기능성 식품 및 소재개발 연구 ... 21
• 제 4 절. 갈매보리수나무의 생리생태적 특성연구 및 재배법 확립 ... 22
• 제 2 장 국내외 기술개발 현황 ... 23
• 제 1 절. 갈매보리수나무 기술개발에 관한 세계적 수준 ... 23
• 제 2 절. 갈매보리수나무 기술개발에 관한 국내적수준 ... 23
• 제 3 절. 갈매보리수나무의 국내·외 연구현황 ... 25
• 제 4 절. 연구개발과제 및 대상기술의 중복성 ... 27
• 제 3 장 연구개발수행 내용 및 결과 ... 28
• 제 1 절. 갈매보리수나무 신품종 및 대량증식 기술 개발 ... 28
• 제 2 절. 갈매보리수나무 기능성 화장품 개발 ... 64
• 제 3 절. 갈매보리수나무를 활용한 고부가가치 기능성 식품 및 소재개발 연구 ... 158
• 제 4 절. 갈매보리수나무 생리생태적 특성 및 재배법 확립 ... 284
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 308
• 제 1 절. 갈매보리수나무 연구를 통한 성과 ... 308
• 제 2 절. 갈매보리수나무 신품종 및 대량증식 기술 개발 ... 311
• 제 3 절. 갈매보리수나무 기능성 화장품 개발 ... 311
• 제 4 절. 갈매보리수나무를 활용한 고부가가치 기능성 식품 및 소재개발 연구 ... 312
• 제 5 절. 갈매보리수나무 생리생태적 특성 및 재배법 확립 ... 313
• 제 5 장 연구개발결과의 활용계획 ... 314
• 제 1 절. 활용계획 ... 314
• 제 2 절. 기대효과 ... 315
• 제 6 장 참고문헌 ... 318