초록 ▼

Ⅱ. 연구개발의 목적 및 필요성
메밀은 곡물중에서 드물게 단백질 함량이 높고 균형잡힌 아미노산구성을 가지고 있다. 또한 다른 곡물에서 볼 수 없는 루틴(rutin)이라고 하는 독특한 기능활성물질이 다량 함유되어 있다. 특히 친환경농산물의 국내 시장규모가 증가하고 있는 점을 감안하면 메밀은 재배 중 농약을 거의 치지 않고 영양학적 측면과 기능성측면에서 다른 곡물에 비해 우수하므로 새로운 농식품 산업화 소재로서 가치가 크다고 할 수 있다. 메밀속 중 식용으로 가능한 종은 보통종과 쓴메밀종이 있는데 쓴메밀종은 자식성이며 루틴함량이

Ⅱ. 연구개발의 목적 및 필요성
메밀은 곡물중에서 드물게 단백질 함량이 높고 균형잡힌 아미노산구성을 가지고 있다. 또한 다른 곡물에서 볼 수 없는 루틴(rutin)이라고 하는 독특한 기능활성물질이 다량 함유되어 있다. 특히 친환경농산물의 국내 시장규모가 증가하고 있는 점을 감안하면 메밀은 재배 중 농약을 거의 치지 않고 영양학적 측면과 기능성측면에서 다른 곡물에 비해 우수하므로 새로운 농식품 산업화 소재로서 가치가 크다고 할 수 있다. 메밀속 중 식용으로 가능한 종은 보통종과 쓴메밀종이 있는데 쓴메밀종은 자식성이며 루틴함량이 높아 금후 새로운 식의약소재로 활용가능성이 매우 크다. 그러나 아직은 시장초기단계이므로 금후 새로운 고부가가치 산업화를 위해서는 쓴메밀 품종개발 및 알려져 있지 않은 새로운 기능성을 탐색하여 소재활용의 기초를 확립할 필요성이 있다. 이에 본 연구는 루틴함량이 높고 다수성인 쓴메밀 품종을 개발하고 쓴메밀이 지닌 새로운 기능성 탐색과 식의약 소재활용을 위한 산업화기술을 개발하는데 그 목적을 두고 수행하였다.
Ⅲ. 연구개발의 내용 및 범위
○ 쓴메밀 유전자원 수집, 특성평가 및 육종효율 증진을 위한 유전분석
○ 쓴메밀 교배육종을 통한 우수계통 선발
○ 쓴메밀 분획물의 항균, 항염, 항암활성 I n vitro 스크리닝
○ 쓴메밀 신 기능성 물질 탐색 및 생리활성 in vivo 효능 구명
○ 쓴메밀 유래 활성물질 분리 및 분리물의 in vivo 효능 및 작용 메카니즘 구명
Ⅳ. 연구개발결과
○ 쓴메밀 육종효율 증진을 위한 SSR 마커 탐색 및 루틴고함유 계통 선발
○ 쓴메밀 생리활성 탐색 및 알츠하이며 예방조성물 특허기술 개발
Ⅴ. 연구개발결과의 활용계획
○ 쓴메밀을 이용한 고부가가치 식의약소재 개발기술의 산업재산권 선점
○ 인지개선능력 효과에 대한 특허출원기술 이전으로 메밀산업 활성화
○ 새로운 식의약소재 개발의 기초자료로 활용

Abstract ▼

Buckwheat is grown throughout a large area of Asia and Southeast Asia as a crop. Buckwheat has a well-balanced amino acid composition that is complementary to cereal grains. Fagopyrum is a genus of about 15 species. common buckwheat (Fagopyrum esculentum L.) and tatary buckwheat (Fagopyrum tataricum

Buckwheat is grown throughout a large area of Asia and Southeast Asia as a crop. Buckwheat has a well-balanced amino acid composition that is complementary to cereal grains. Fagopyrum is a genus of about 15 species. common buckwheat (Fagopyrum esculentum L.) and tatary buckwheat (Fagopyrum tataricum G.) are the most important species in the genus. Many studies on tatary buckwheat have been trying recently, especially for improving higher nutritional value of common buckwheat. Tatary buckwheat probably originated in northwestern Yunnan in China, and this species is now cultivated in the high-altitude mountainous areas of Asia and to a much lesser extent elsewhere in Europe. The flowers are homomorphic and self-fertile, with pollination occurring before the flower opens. Studies on tatary buckwheat were increased due to tatary buckwheat containing many micro-elements, vitamins and vitamin B, and being especially rich in antioxidants, such as rutin and quercetin. Rutin in buckwheat is a kind of flavonol glycoside compounds used in preventing edema, haemorrhagic diseases, and stabilizing high blood pressure due to its effectiveness in controlling blood vessel. This experiment was to select tatary buckwheat lines with high yield and rutin content. We evaluated 75 tatary buckwheat lines(or varieties) at lowland and highland on summer and autumn, respectively. There were a positive correlation between branch number and flower clusters and yield. Onthe other hand, plant height and days to flowering were shown negative correlation with yield. 9 lines among the selected 12 lines with high yield were shown higher rutin content than standard cultivar. 36 F1 hybrids obtained at 125 crossing combinations. After evaluation of agronomical traits of F1 of 36 crossing lines, 9 lines of summer type and 14 lines of autumn type.
Phytochemical constituents were isolated from the seeds of Fagopyrum tataricum (Polygonaceae) by open column chromatograph. Their structures were elucidated as β-sitosterol, β-sitosterol-3-O-glucoside, oleanolic acid, kaempferol, quercetin, kaempferol-3-O-rutinoside, and quercetin-3-O-rutinoside. The protective effects from oxidative stress of fractions and rutin from Fagopyrum tataricum were evaluated. The treatment of n-hexane, methylene chloride, ethyl acetate, and n-butanol fractions from F. tataricum showed over 90% scavenging effect against DPPH at 500μ g/ml. In addition, the fractions showed over 80% ㆍOH scavenging activity at the concentration of 50μg/ml. Moreover, rutin showed over 80% scavenging effect against DPPH at 50μg/ml. ㆍOH scavenging activity of rutin showed over 80% at 10μg/ml. Furthermore, the treatment of H2O2 to C6 glioma cell induced oxidative stress, however the fractions from F. tataricum and rutin increased significantly the cell viability. Protective effects of Tartary buckwheat (TB) and common buckwheat (CB) on amyloid beta (Ab)-induced impairment of cognition and memory function were investigated in vivo in order to identify potential therapeutic agents against Alzheimer’s disease (AD) and its associated progressive memory deficits, cognitive impairment, and personality changes. An in vivo mouse model of AD was created by injecting the brains of ICR mice with Ab25–35, a fragment of the full-length Ab protein. Damage of mice recognition ability through following Ab25–35 brain injections was confirmed using the T-maze test, the object recognition test, and the Morris water maze test. Results of behavior tests in AD model showed that oral administration of the methanol (MeOH) extracts of TB and CB improved cognition and memory function following Ab25–35 injections. Furthermore, in groups receiving the MeOH extracts of TB and CB, lipid peroxidation was significantly inhibited, and nitric oxide levels in tissue, which are elevated by injection of Ab25–35, were also decrease. In particular, the MeOH extract of TB exerted a stronger protective activity than CB against Ab25–35-induced memory and cognition impairment. The purpose of this study is to elucidate the molecular mechanism of anti-inflammatory effect of the fractions (n-hexane, methylene chloride, ethyl acetate, and n-butanol) and active compound, rutin, which was isolated from Fagopyrum tartaricum. We investigated the protective activity of the fractions and rutin against NO-induced oxidative damage by sodium nitroprusside (SNP) in RAW264.7 macrophages. We further investigated the effects of the fractions and rutin on the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nuclear factor- κB (NF-κB) under RT-PCR in SNP-stimulated RAW264.7 cells. The fractions and rutin suppressed the NO production and down-regulated the levels of iNOS, COX-2 and NF-κB mRNA expressions. The present study indicate that the fractions and rutin from F. tartaricum showed protective activity against oxidative damage induced by NO hrough regulation of its related mRNA expressions such as iNOS, COX-2 and NF-κB.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 4
• 목차 ... 6
• 제 1 장 서 론 ... 7
• 1절 기능성 물질 고함유 쓴메밀 계통 육성 ... 7
• 2절 쓴메밀의 인체생리활성탐색 및 질환개선효능 구명 ... 7
• 제 2 장 국내외 기술개발 현황 ... 9
• 1절 기능성 물질 고함유 쓴메밀 계통 육성 ... 9
• 2절 쓴메밀의 인체생리활성탐색 및 질환개선효능 구명 ... 10
• 제 3 장 연구개발수행 내용 및 결과 ... 12
• 1절 기능성 물질 고함유 쓴메밀 계통 육성 ... 12
• 2절 쓴메밀의 인체생리활성탐색 및 질환개선효능 구명 ... 28
• 제 4 장 연구개발목표 달성도 및 대외기여도 ... 64
• 1절 목표대비 대외 달성도 ... 64
• 2절 정량적 성과 ... 65
• 제 5 장 연구개발결과의 활용계획 ... 68
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 69
• 제 7 장 기타 중요 변동사항 ... 70
• 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 71
• 제 9 장 참고문헌 ... 72