초록 ▼

Ⅲ. 사업(연구개발)의 내용 및 범위
- 자체적으로 확보한 호열균(Thermus caldophilus GK 24/ Caldicellulosruptor owensis) 유전체 정보를 분석하고, 이로부터 관련 효소유전자(alpha-glucan phosphorylase, phophoglucomutase, glucose-6P isomerase, phophofructokianse, FB aldolase, transaldolase,transketolase 등) 발굴.
- 내열성 효소 유전자들의 발현과 효소변이유도 실험.
- 해

Ⅲ. 사업(연구개발)의 내용 및 범위
- 자체적으로 확보한 호열균(Thermus caldophilus GK 24/ Caldicellulosruptor owensis) 유전체 정보를 분석하고, 이로부터 관련 효소유전자(alpha-glucan phosphorylase, phophoglucomutase, glucose-6P isomerase, phophofructokianse, FB aldolase, transaldolase,transketolase 등) 발굴.
- 내열성 효소 유전자들의 발현과 효소변이유도 실험.
- 해당효소와 변이효소의 반응을 HPLC 검증하고 분리하고, 또 NMR과 Mass분석기술의 활용하여 구조분석을 수행.
- 변이효소반응의 응용과 C3/C5/C7 당류의 제조 진행.

Abstract ▼

The synthesis of heptulose in this system was tentatively explained according to three different hypotheses. The first was based on the presence of residual catalytic quantities of acceptor in the enzyme preparation; the second postulated the transfer of glycolaldehyde to D-glucose 6-phosphate to fo

The synthesis of heptulose in this system was tentatively explained according to three different hypotheses. The first was based on the presence of residual catalytic quantities of acceptor in the enzyme preparation; the second postulated the transfer of glycolaldehyde to D-glucose 6-phosphate to form octulose S-phosphate, thus generating D-erythrose 4-phosphate; and the third suggested a coupled attack by transketolase and transaldolase upon 2 molecules of D-fructose 6-phosphate, each enzyme cleavage reaction producing the acceptor for the other enzyme. We demonstrate that the process proceeds as well in the complete absence of hexose phosphate isomerase and support the third hypothesis, namely that of a coupled reaction catalyzed by transketolase and transaldolase. We have observed that D-frUCtose 6-phosphate, completely free of D-glucose B-phosphate, is converted to sedoheptulose 7-phosphate and n-xylulose 5-phosphate with preparations of transaldolase and transketolase which are highly purified and completely free of hexose phosphate isomerase and n-xylulose 5-phosphate 3-epimerase activity.

목차 Contents

• 표 지 ... 1
• 제 출 문 ... 2
• 기관고유사업보고서 초록 ... 3
• 요 약 문 ... 4
• SUMMARY ... 7
• CONTENTS ... 7
• 목 차 ... 8
• 제 1 장 서론 ... 8
• 제 2 장 국내.외 기술개발 현황 ... 8
• 제 3 장 사업(연구개발)수행 내용 및 결과 ... 9
• 제 4 장 사업(연구개발)목표 달성도 및 대외기여도 ... 26
• 제 5 장 연구개발결과의 활용계획 ... 26
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 26
• 제 7 장 참고문헌 ... 26