초록

본 연구는 편향을 예방하기 위한 일련의 은폐를 실시하고 타당한 실험동물 개체수를 확보한 MPTP-유도 파킨슨병 동물모델에서 정량적으로 침치료에 의한 도파민성 신경세포의 사멸 억제 효과를 확인하기 위해, (1) MPTP 대조군, (2) MPTP 침처치군, (3) MPTP 위약(placebo)침처치의 세 군에서 면역조직 화학 염색을 통해 TH 면역 양성세포의 비율을 측정 비교하여 침처치군에서 MPTP에 의한 흑색질의 TH 면역반응 양성 세포의 감소가 유의하게 억제되었는지 확인하고자 하였음.

Abstract ▼

Neuronal changes with acupuncture treatment for patients with Parkinson's disease has not been well established, although some clinical and experimental studies suggest that acupuncture treatment may produce a neuroprotective effect and restore dopaminergic and motor functions.
The purpose of th

Neuronal changes with acupuncture treatment for patients with Parkinson's disease has not been well established, although some clinical and experimental studies suggest that acupuncture treatment may produce a neuroprotective effect and restore dopaminergic and motor functions.
The purpose of this study was to determined the effects of acupuncture on the nigrostriatal dopaminergic neurodegeneration in the mouse model of Parkinson's disease(PD).
Forty-five male C57BL/6 mice were used. Parkinsonism was induced by intraperitoneal injecting with 5 doses(Every 24 hour) of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) 30 mg/kg over consecutive 5 days. Mice were divided into 3 groups.
(1) Control group (n=15)
(2) Acupuncture treatment group (n=15)
(3) Placebo acupuncture treatment group (n=15)
As to acupuncture treatment group, acupuncture needles with 0.2 mm in diameter were inserted into the GB34 (陽陵泉) acupuncture points. Placebo acupuncture treatment group was also inserted by stainless acupuncture needle about 3mm in depth into both hips. 12th days after MPTP intraperitonral injection, mice were sacrificed. The animals in terminal anesthesia were transcardially perfused with 4% paraformaldehyde(PFA) in 0.1M phosphate buffer(PB) followed by an overnight immersion in the same fixative solution at 4C. The brains were removed, post-fixed, and cryoprotected. We collected sections 20um sections for TH immunohistochemistry and the numbers of stained cellular elements will be counted using image-analysis based cell counting.
Recent studies suggest the possible role of acupuncture in the treatment of Parkinson's disease. Acupuncture has been reported to inhibit the neurotoxic inflammatory events and the degeneration of dopaminergic cells. For this study, we used a subacute mouse model of Parkinsonism. We plan to analysis via TH immunohistochemistry the survival of dopaminergic neurons in the substatia nigra (SN) and the neuroprotective effects against neuronal death in the PD animal model.

목차 Contents

• 표지 ... 1
• 제출문 ... 3
• 보고서 요약서 ... 4
• 요 약 문 ... 5
• SUMMARY ... 6
• 6. 연구 성과 실적 및 향후 계획 ... 7
• 7. 참여연구원 현황표 ... 11
• [별지 제3호 서식] 보건의료기술진흥사업 최종보고서 서식 ... 12
• II. 총괄연구과제 연구결과 ... 14
• 1. 연구개발과제의 배경 및 필요성 ... 15
• 2. 국내외 기술개발 현황 ... 15
• 3. 연구개발과제의 추진체계 ... 16
• 4. 연구개발수행 내용 및 결과 ... 17
• 5. 목표달성도 및 관련분야 기여도 ... 19
• 6. 향후 연구성과 추진 계획(※해당되는 경우만 작성) ... 20
• 7. 연구개발결과의 파급효과 ... 20
• 8. 연구개발결과의 활용계획 ... 21
• 9. 연구개발과정에서 수집한 해외과학기술정보 (해당 없음) ... 22
• 10. 참고문헌 ... 22
• IV. 첨부서류 ... 24
• [ 양식 4 ] [국가연구시설장비등록 확인 : 본 과제로 구축한 연구시설․장비를 작성] ... 25
• 끝페이지 ... 26