초록 ▼

Ⅴ. 연구개발결과의 활용계획
1. 항생제 대체물질 연구가 전 세계적으로 활발히 진행되고 있으며, 특히 EU국가에서 연구 중인 식물추출물을 활용한 질병치료 대체물질에 대한 실증평가 필요
가. 유기산제, 생균제, 효소제, 생리활성물질, 에센션오일 등
2. 항생제 대체요법으로 생리활성물질 또는 면역증강물질을 투여에 의한 면역원성 증진에 관한 연구 수행필요
가. 화분(bee pollen), 다당류(베타글루칸, 락토페린 등), Phytochemical 등
3. 산업화를 위한 천연항생물질 및 면역증강물질의 추출기술

Ⅴ. 연구개발결과의 활용계획
1. 항생제 대체물질 연구가 전 세계적으로 활발히 진행되고 있으며, 특히 EU국가에서 연구 중인 식물추출물을 활용한 질병치료 대체물질에 대한 실증평가 필요
가. 유기산제, 생균제, 효소제, 생리활성물질, 에센션오일 등
2. 항생제 대체요법으로 생리활성물질 또는 면역증강물질을 투여에 의한 면역원성 증진에 관한 연구 수행필요
가. 화분(bee pollen), 다당류(베타글루칸, 락토페린 등), Phytochemical 등
3. 산업화를 위한 천연항생물질 및 면역증강물질의 추출기술 확립과 경제적 제품생산기술 연구 수행 요구
가. 베타글루칸 생산 및 정제기술 (대한민국 특허공개번호 1999-028736)
나. 화분내 다당류 및 식물내 Phytochemical 추출기술, 에센셜 오일 정제기술 등
다. 미국 등의 경우 높은 농도의 병원체 특이 항체 및 림프구를 포함한 초유 및 혈장의 수동적인 투여가 병원체를 효과적으로 방어한다는 것을 증명하고 다양한 병원체 특이 초유 및 혈장 제품들을 생산하고 있음
라. 국외의 경우에도 송아지 설사병 병원체에 특이적인 제품들은 아직 개발되어 있지 않음
마. 국내 송아지 설사병의 주요 병원체들을 조사하고 이들 병원체에 특이적인 항체 및 림프구를 고도로 가진 혈장 및 초유를 생산하고 이들의 설사병 치료효과를 평가할 필요가 있음

Abstract ▼

It has been reported that gastrointestinal disorder is the most frequent diagnosis in calves and caused the most economically significant problem in Korean cattle farms.
Although various pathogens could be major causative factors in calf diarrhea, insufficient uptake of colostrum is one of the mo

It has been reported that gastrointestinal disorder is the most frequent diagnosis in calves and caused the most economically significant problem in Korean cattle farms.
Although various pathogens could be major causative factors in calf diarrhea, insufficient uptake of colostrum is one of the most important factor. Because colostrum is considered to be the sole source of passively acquired maternal antibodies for calves, newborn calves must consume colostrum to gain disease resistance during their early years of life.
Colostrum banks that collect and save surplus colostrum from neighboring dairy farms and provide colostrum for farms where colostrum is insufficient to feed new born claves have been operated in many Agricultural Technique Centers (ATCs) in Korea. The biggest problem in this system, however, is disease transmission because various pathogens can be contaminated in colostrum and it could be the major source for disease spread between farms. In addition, the quality of colostrum is important to achieve a good level of protection against various diseases related to newborn mortality. In fact, the IgG concentrations of the colostrums collected from ATCs ranged from 8.57 to 96.74 g/L while IgA concentration ranged from 0.98 to 31.18 g/L. Only 16 out of 35 colostrums contained equal or higher than 30 g/L of IgG, which is the minimum concentration for adequate quality colostrums and only 10 were considered good quality colostrums which contained equal or higher than 50 g/L of IgG. BVDV-specific antibody was detected only from 5 colostrums, indicating that most of the colostrums were not collected from cows frequently exposed to BVDV infection. A great deal of bacterial colonies was observed from most of the colostrums (up to 7.2x10⁷CFU/ml). To evaluate the effect of pasteurization on the colostrums, all of the colostrums were incubated at63℃ for 30 min in a water bath. The average number of bacterial cells counted from general bacterial culture after pasteurization was less than 10²CFU/ml.When IgG or IgA levels before and after pasteurization were analyzed by pair-wisecomparison,the decrease of IgA concentration after pasteurization was consistent throughout the collected colostrums and statistically significant (p<0.01). On the other hand, the decrease of IgG concentration after pasteurization was not statistically significant (p>0.1). Therefore, it was recommended that colostrum should be heat-treated before frozen and stored in the bank because pasteurization at 63℃ for 30 min was very effective reducing the risk of disease transmission without causing significant degradation of immunoglobulins.
We investigated that effects of IGF and lactoferrin, the possible effective component of colostrum in human neuronal cells. Lactoferrin (LF) is an 80 kDa protein, which has antioxidant abilities due to the scavenging of ROS. The effects of LF treatment on PrP (106-126)-mediated neurotoxicity and ROS generation were the focus of this study. LF treatment protected against PrP (106-126)-induced neuronal cell death and decreased ROS
generation. The reduced ROS generation prevented PrP (106-126)-induced mitochondrial dysfunction. Moreover, PrP (106-126)-induced protein activation including c-Jun N-terminal kinase and caspase-3 were blocked by LF treatment. These results demonstrated that LF protects neuronal cells against PrP (106-126)-mediated neurotoxicity through the scavenging of ROS and provide evidence that LF treatment prevents neuronal cell death caused by PrP (106-126). Insulin-like growth factor-1 (IGF-1) is one of the most important components of bovine colostrum. It exhibits antiapoptotic and antioxidative activities. This study examined the protective effect of IGF-1 on residues 106-126 of the cellular prion protein [PrP (106-126)]-mediated mitochondrial neurotoxicity and oxidative stress. In SH-SY5Y human neuronal cells, treatment with PrP (106-126) decreased the cell viability and IGF-1 pretreatment markedly blocked the PrP (106-126)-induced neuronal cell death. IGF-1 inhibited PrP (106-126)-induced intracellular ROS generation and mitochondrial oxidative stress. In addition, IGF-1 blocked the translocation of the Bax protein to the mitochondria induced by PrP (106-126). These results demonstrate that IGF-1 protects neuronal cells against PrP (106-126)-mediated neurotoxicity through an antioxidative effect and blockage of mitochondrial Bax translocation. The results also suggest that regulation of IGF-1 secretion may have a therapeutic potential in the management of mitochondrial dysfunction and oxidative stress-induced neurodegeneration.
The use of herbal medicines was also evaluate for the effects on preventing calf diarrhea in the current study. Thirty three herbal plants were screened based on their medical functions such as antimicrobial, antidiarrhea, astringent and antiinflamation effects. Four herbal plants (Aralia cordata, Myristica fragrans, Sanguisorba officinalis and Terminalia chebula) were finally selected in terms of cytotoxicity, antimicrobial activity and immune enhancement effect. Cytotoxicity was not observed on hot water extracts from Aralia cordata, Myristica fragrans, Sanguisorba officinalis and Terminalia chebula. Hot water extracts from Aralia cordata and Myristica fragrans had cell proliferation effect. Especially, Aralia cordata extract activated immune cells and enhanced Nitric oxide production. Twenty nine percent of diarrhea was reduced from the calves that had fed four herbal extracts combination (Aralia cordata, Myristica fragrans, Sanguisorba officinalis and Terminalia chebula). In conclusion, hot water extract of four herbal plants reduced the calf diarrhea and promote immune cells function. Further study on young animal growth effect is recommended for using of those herbal plants as growth promoter.

목차 Contents

• 표 지 ... 1
• 제출문 ... 2
• 요 약 문 ... 3
• SUMMARY ... 6
• 목 차 ... 8
• 제 1 장 서 론 ... 9
• 1절. 연구개발대상 기술의 경제적 • 산업적 중요성 및 연구개발의 필요성 ... 9
• 2절. 연구 배경 ... 10
• 제 2 장 국내외 기술개발 현황 ... 11
• 1절: 국내 연구 현황 ... 11
• 2절: 국외 연구 현황 ... 13
• 제 3 장 연구개발수행 내용 및 결과 ... 16
• 제1세부과제 : 국내 초유은행 운영 시스템 구축 및 활용에 관한 연구 ... 16
• 제2세부과제 : 고도면역 혈장 및 초유를 활용한 항생제 대체제 연구 및 개발 ... 39
• 제3협동과제 : 다당류 및 식물추출물 이용 송아지 설사치료용 항생제대체제 개발 ... 66
• 제 4 장 연구개발 목표 달성도 및 대외 기여도 ... 85
• 1절 목표대비 달성도 ... 85
• 2절 : 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 86
• 제 5 장 연구개발결과의 활용계획 ... 87
• 1절. 국내외 연구현황 비교 및 필요 연구 분야 ... 87
• 2절. 현재 추진중인 논문, 산업재산권 현황 ... 87
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 88
• 제 7 장 기타 중요 변동사항 ... 90
• 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 90
• 제 9 장 참고문헌 ... 90
• 끝페이지 ... 92