구제역 백신 다수 접종에 의한 NSP (비구조단백질) 항체 형성 여부 조사 Serological immune responses against NSP residues in FMD trivalent vaccine after multiple vaccination in cattle, pigs or goats원문보기
보고서 정보
주관연구기관
농림축산식품부 Ministry of Agriculture, Food and Rural Affairs
보고서유형
최종보고서
발행국가
대한민국
언어
한국어
발행년월
2014-04
과제시작연도
2013
주관부처
농림축산식품부 Ministry of Agriculture, Food and Rural Affairs(MAFRA)
등록번호
TRKO201400012393
과제고유번호
1545006244
사업명
농림축산검역검사기술개발
DB 구축일자
2014-07-05
키워드
구제역.백신.다수접종.FMD.Vaccine.NSP.multiple vaccination.
초록▼
최종 연구결과 요약 ◦세부과제명: 구제역 3 가 백신 다수 접종에 따른 NSP 항체 형성 조사 - 구제역 3가 백신 접종 소, 돼지, 염소에서의 NSP 항체 형성 여부 조사 ◦ 시험·접종군 선정 및 백신접종 상황 가. 축종별 시험군 선정 : 소 62두 (3 농가), 돼지 24두 (2 농가), 염소 45두 (2 농가) 나. 백신제조사별 접종군 : 소, 염소 (A사 및 B사), 돼지 (A사) 다. 백신 접종 : 2 개월령에 1차 접종 후 한 달 간격으로 12차까지 접종(2014년 3월말 14개월령으로 1
최종 연구결과 요약 ◦세부과제명: 구제역 3 가 백신 다수 접종에 따른 NSP 항체 형성 조사 - 구제역 3가 백신 접종 소, 돼지, 염소에서의 NSP 항체 형성 여부 조사 ◦ 시험·접종군 선정 및 백신접종 상황 가. 축종별 시험군 선정 : 소 62두 (3 농가), 돼지 24두 (2 농가), 염소 45두 (2 농가) 나. 백신제조사별 접종군 : 소, 염소 (A사 및 B사), 돼지 (A사) 다. 백신 접종 : 2 개월령에 1차 접종 후 한 달 간격으로 12차까지 접종(2014년 3월말 14개월령으로 12차 백신 접종 후 4주 경과) ◦ NSP 항체 형성 여부 조사 결과 가. 돼지에서는 전 두수 음성, 소는 6두, 염소는 7두 양성 나. 소, 염소의 NSP 항체 양성 개체에 대한 구제역바이러스 검출결과 음성 ◦ SP 항체 형성 여부 조사 결과 A 및 B사 백신(O1 Manisa, A Malaysia, Asia1 Shamir) 모두 항체 형성 확인
Abstract▼
Foot and mouth disease (FMD) is the most contagious disease of cloven-hoofed mammals, and cause huge economic losses of the farmers and country. Detection of antibodies to non-structural protein (NSP) has been used to identify past or ongoing infections with any of the existing serotypes of FMDV. Si
Foot and mouth disease (FMD) is the most contagious disease of cloven-hoofed mammals, and cause huge economic losses of the farmers and country. Detection of antibodies to non-structural protein (NSP) has been used to identify past or ongoing infections with any of the existing serotypes of FMDV. Since severe damages due to FMD outbreaks in South Korea from November 2010 to April 2011, South Korea adopted vaccination policy throughout the country for all susceptible animals. For the purpose of finding infected animals by detecting humoral immune responses to NSP of the FMD virus, antibodies induced by contaminated minute NSP contained in less pure FMD vaccine can be problematic for serological screening. This study was aimed to see whether antibodies against NSP could be induced in repeatedly vaccinated cattle, pigs and goats. From March 2013, approximately 131 cattle, pigs and goats were kept on seven farms in Korea. At the beginning of this study, all of the animals were approximately 6 weeks of age. All animals were vaccinated once a month from at 8 weeks of age with oil adjuvanted, inactivated purified FMD trivalent vaccine against FMD types O, A and Asia 1 (Merial Animal Health Ltd or Intervet Co.). Blood samples were collected by jugular vein puncture at the time of vaccination and monthly thereafter. Those sera were tested by NSP ELISA (Median Diagnostics, Bionote, Korea; PrioCHECK® FMDV NSP, Netherlands) and SP ELISA(PrioCHECK® FMDV type O, Netherlands; Pirbright LPB A and Pirbright LPB Asia1, United Kingdom) according to the manufacturer’ instructions. All animals were 14 month old and vaccinated 12 times. NSP antibodies were not detected in all animals except for 6 cattle and 7 goats which were tested positive. However, the NSP-seropositive cattle and goats showed negative results in antigen test by real-time RT-PCR. The SP-positive prevalence was increased in all three types depending on receiving vaccination times and the prevalence of type O and of Asia1 showed slightly higher levels than that of type A. We found that a multiple vaccinated animal showed positive in serological NSP assay. At this stage, we do not precisely know why NSP antibody was induced in animals. However some explanations of our result would be non-specificity of the assay or induction by minute NSP-contaminated vaccines. It was unlikely that the positive reaction tested in the field sample was caused by natural FMD virus infection. Currently, there is no confirmatory diagnostic method to support NSP ELISA for detection of true NSP antibodies induced by wild type FMD viruses. Thus, further study is required to develop the additional methods for confirmation of suspicious NSP-positive samples.
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