초록 ▼

- 꽃송이버섯 신품종 체취 및 육성 완료
- 꽃송이버섯 균사체의 대량증식 기술개발 및 신품종 육성균주 자실체 발생 검증
- 액체탱크 배양 조건 구명 완료
- 엘리시터 첨가에 따른 베타글루칸 함량을 증량조건 구명완료
- 꽃송이와 표고를 중심으로 버섯 추출물의 항산화능/항당뇨/항암/항염 기능성 확보
- 꽃송이 표고버섯 추출물중 소수성 유용성분 분리를 위한 HPLC 및 분취용 HPCCC 조건 확립
- 꽃송이 표고버섯 추출물중 친수성 유용성분 분리 완료 (렉틴 및 효소 2종)
- 꽃송이 버섯 분말을 이

- 꽃송이버섯 신품종 체취 및 육성 완료
- 꽃송이버섯 균사체의 대량증식 기술개발 및 신품종 육성균주 자실체 발생 검증
- 액체탱크 배양 조건 구명 완료
- 엘리시터 첨가에 따른 베타글루칸 함량을 증량조건 구명완료
- 꽃송이와 표고를 중심으로 버섯 추출물의 항산화능/항당뇨/항암/항염 기능성 확보
- 꽃송이 표고버섯 추출물중 소수성 유용성분 분리를 위한 HPLC 및 분취용 HPCCC 조건 확립
- 꽃송이 표고버섯 추출물중 친수성 유용성분 분리 완료 (렉틴 및 효소 2종)
- 꽃송이 버섯 분말을 이용한 제빵 개발 및 버섯 혼합분말을 첨가한 기능성 김치 제조 완료
- 꽃송이 버섯 추출물을 이용한 화장품 원료 등재 및 화장품 제품 개발 완료 (매출 발생)
- 표고버섯 건강기능식품 등재를 위한 추출조건 확립/기초분석/독성검사/활성분석
- 꽃송이 버섯 원물이 포함된 건강기능식품 고시형 1종 개발 (유산균과 혼합제품)

Abstract ▼

The use of mushrooms has immense potential in many diverse applications. They have been used in forestry, industry, biotechnology, bioremediation, restoration, and reforestation. They have been consumed as food and medicine since ancient times due to their enormous health benefits. It is estimated t

The use of mushrooms has immense potential in many diverse applications. They have been used in forestry, industry, biotechnology, bioremediation, restoration, and reforestation. They have been consumed as food and medicine since ancient times due to their enormous health benefits. It is estimated that there are 140,000 mushroom species on the earth and nearly 10% are known (Wasser, 2002). Mushrooms can improve one’s health significantly because they are low in calories, sodium, fat and cholesterol;they also contain a large amount of protein, carbohydrate, fiber, vitamins and minerals. All together, these nutritional properties make mushrooms part of a well-balanced diet (Manzi et al., 1999). More than 3,000 species are consumed around the world, and more than 100 have shown promising clinical activity against cancer and other chronic diseases. In the food and pharmaceutical industries, some useful mushrooms are in demand (Garibay-Orijel et al., 2009). Sparassis crispa (now known as S. latifolia) is also commonly called the cauliflower mushroom in English, Hanabiratake in Japanese and Ggoksongee (meaning a blossom) in Korean. The scientific nameisWulfen:Fries,which was derived from their curly or crisped branches. S. crispa is a good source of bioactive natural products that have antiviral and anticancer properties and also has a rich flavor.
In this research report, based on R&D project of the 4 year research project, we reported several outcomes from distribution and taxonomy to industrial application of commercial products. According to the investigations of the ecological condition of the S. crispa productive sites, the isolation sites showed a very wide altitudinal distribution, ranging from 240 m to 1,100 m, and were found especially in very high altitudes (>1,000 m). In general, the mushrooms showed distinct features of heart-rot fungus, as it was found on soils around the stems of larches (Larix kaempferi ) and Korean white pines (Pinus koraiensis). However, some of the mushrooms could be found on the stem or stump of dead trees, which indicated that the fungus might have several invasion routes and capabilities to grow on various ecological conditions (Oh, 2009). S. crispa’s mycelium and fruiting body can be cultured using solid and liquid substrates by three methods: bottle, bag and log cultivation. In the bottle cultivation method, neither the sawdust nor the liquid as an inoculum showed significant differences for the growth of mycelium. Several new strains were isolated throughout the countries and mass cultivation technologies of fruiting body and mycelium were developed and commercially transferred to several companies.
The hot water extracts of the fruiting bodies of S. crispa are specifically useful for the application of the traditional natural remedies. Ohna et al.(2000) reported 52.0% of β-glucan content by the hot water, cold alkaline and hot alkaline extraction in S.crispa. The larger molecular compounds were precipitated from the crude water extracts by methanol. Until now, approximately 20 different major diverse classes of natural products from S.crispa mushroom were noted, which can be put into practice in medicinal research (Politi et al., 2007). Recently, the molecular weight of the S.crispa polysaccharides from the extraction was 510 kDa, and the average particle size diameter was 150±14.1 nm. These fine particles are useful for industrial applications and drug delivery because they are easily absorbed by the human intestine. They also reported that the alkaline extraction and hot water extraction method gave a β-glucan content of 70.2%. In this report, we have made extracts using 80% ethanol and subsequent column chromatography resulting in several fractions. The fractions were applied to several in vitro tests including anti-oxidant, anti-diabetes, and anti-cancer activities. Some animal studies were done to verify the weight-loss effect and anti-cancer effects using mouse and zebrafish. The fractions were further purified to make a pure compound by DEAE-Sehparose, HPLC and high-performance counter current chromatography (HPCCC) technique. One lectin and two enzymes were firstly isolated and purified. Also several small molecules were separated by HPLC-MS technique.
The dried fruiting bodies of the S. crispa yielded a novel compound which prevents melanin biosynthesis and methicillin-resistant Staphylococcus aureus (MRSA) growth. The IC50 value for the melanin production inhibition assay was 12 μM and the MIC value against MRSA was 1.0 mM (Kawagishi et al., 2007). In this report, we have developed several commercial cosmetics using the dried fruiting bodies and the extracted fractions. Several million dollars of revenue were made by exporting the cosmetic products. Besides, a number of food and nutraceutical products were developed as follows: normal side dish using fresh mushroom fruiting bodies, bread and Kimchi, and nutraceuticals.
In summary, primary technology about isolation, cultivation, extraction, and purification of S. crispa and its extracts was developed. And this technology could be used as a good starting point of much more specialized medical products. The contents of the report would be beneficial eventually for farmers raising S. crispa mushroom throughout the contries.

목차 Contents

• 제 출 문 ... 1
• 보고서 요약서 ... 2
• 요 약 문 ... 3
• SUMMARY ... 5
• CONTENTS ... 8
• 제 1 장 연구개발과제의 개요 ... 10
• 제 1 절 연구의 필요성 ... 10
• 제 2 절 연구개발의 목표 및 내용 ... 11
• 제 2 장 국내외 기술개발 현황 ... 15
• 제 1 절 국내 기술개발 현황 ... 15
• 제 2 절 국외 기술개발 현황 ... 37
• 제 3 장 연구 개발 수행 내용 및 결과 ... 53
• 제 1 절 제1세부과제: 전남산림자원연구소 ... 53
• 제 2 절 제2세부과제: 조선대학교 산학협력단 ... 98
• 제 3 절 제3세부과제: 파고다 ... 159
• 제 4 절 제3세부과제: (주)컬러핑크알앤디 ... 197
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 243
• 제 1 절 평가의 착안점 ... 243
• 제 2 절 목표의 달성도 ... 244
• 제 3 절 관련분야의 기여도 ... 245
• 제 5 장 연구개발결과의 활용계획 ... 246
• 제 1 절 연구개발결과의 활용방안 ... 246
• 제 2 절 기대성과 ... 246
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 248
• 제 7 장 참고문헌 ... 257
• 끝페이지 ... 291