초록 ▼

○ 연구결과
·채엽전 다원에서 자외선과 methyl jasmonic acid 1,000ppm 조합처리로 가바함량을 대조구에 비해 10% 향상시켰음
·제다전 100% 질소 개스 혐기와 호기 교호처리로 차 외관과 맛을 저하시키지 않으면서 가바함량을 대조구에 비해 300% 향상시켰음
·가바차 동물 식이와 성인에서 음용시 혈압과 체중 감소, 지질대사 개선으로 가바차는 특히 항비만에 우수하다는 사실이 밝혀졌음
·가바차 주요성분에서 건물중 g당 8.6mg의 가바가 함유되어 있었음
·가바차와 티백, 가루차와 캔음료,

○ 연구결과
·채엽전 다원에서 자외선과 methyl jasmonic acid 1,000ppm 조합처리로 가바함량을 대조구에 비해 10% 향상시켰음
·제다전 100% 질소 개스 혐기와 호기 교호처리로 차 외관과 맛을 저하시키지 않으면서 가바함량을 대조구에 비해 300% 향상시켰음
·가바차 동물 식이와 성인에서 음용시 혈압과 체중 감소, 지질대사 개선으로 가바차는 특히 항비만에 우수하다는 사실이 밝혀졌음
·가바차 주요성분에서 건물중 g당 8.6mg의 가바가 함유되어 있었음
·가바차와 티백, 가루차와 캔음료, 파우다 제조공정 확립과 함께 4종 시제품을 개발하였음

Abstract ▼

This experiment has 2 main purposes: one is to develop the gaba enhancement system in green tea, the other is to establish the suitable production procedure for gaba tea. This study consist of 8 parts which have objectives are as follow: 1) to enhance gaba content in green tea trees by using light s

This experiment has 2 main purposes: one is to develop the gaba enhancement system in green tea, the other is to establish the suitable production procedure for gaba tea. This study consist of 8 parts which have objectives are as follow: 1) to enhance gaba content in green tea trees by using light source and signal transfer material, 2) to increase gaba content by various pretreatment such as microorganism, 3) to rise gaba content by combination of anarchic and aerobic treatments before processing, 4) to investigate the protective effectiveness of hypotension in rats fed with gaba tea, 5) to investigate the protective effectiveness of lipid metabolism in human consumed gaba tea, 6) to analysis major components and nutraceutical content in both gaba tea and its extracts, 7) to extend shelf life of gaba tea during storage, 8) to produce value added gaba tea product and panel test by comsumer.
For the gaba enhancement in the first part, we analysed major components and gaba content in tea trees by different light wavelengths and signal transfer materials in field condition. Any harmful effects did not find in tea trees by both treatments during 2006-2007 seasons. Also, we can not found any changes of shoot growth and major components either in light source or signal transfer material treatments. Gaba and GDC activity were much increased in either ultraviolet or methyl jasmonate compared with control. Gaba content is significantly higher in combination treatment with ultraviolet and signal transfer material comparison with single treatment.
The second part was conducted to increase the gaba content in tea leaves by various pretreatments before processing. In water soaking treatment, gaba content slightly increased in either at $0^{\circ}C$ for 4 hours or at $20^{\circ}C$ for 2 hours treatments compared with lion-treatment. In producing of gaba content by various microorganism, Lactovacillus brevis influenced the highest gaba content among microoranism in vitro under grown 2% glutamic acid.
The third part was conducted to increase gaba content by anaerobic treatment in tea leaves. An anaerobic treatment, gaba content is much higher at $20^{\circ}C$ for 3 hours than those of at $10^{\circ}C$ or $30^{\circ}C$ treatments. Also, gaba content significantly increased with combination treatments of anaerobic and aerobic at $20^{\circ}C$.
The fourth part was conducted to investigate the changes of catechins in $\gamma$-aminobutyric acid (GABA)-rich green tea (GRGT) and its anti-obesity effect. The content of catechins in GRCT were significantly higher than those of green tea. In particular, catechin (C) and epigallocatechin gallate (EGCG) greatly increased over 1.5 folds during the manufacturing of GRGT. Also, lipase inhibitory activity was shown to be higher in GRGT than green tea (GT).
GRGT was administered to male diabetic Sprague-Dawley rats with high fat diet for four weeks. Body weight gain and lipid level in plasma of GABA tea and GT groups were significantly lower than those of control group (P<0.05). Total-abdominal adipose tissue weight in the GRGT group was significantly lower than that in control and GT groups (p<0.05). The levels of plasma triglyceride and plasma total-cholesterol in the GRGT group were significantly lower than other groups (p<0.05). Plasma low-density lipoprotein cholesterol in GRGT and GT groups were significantly lower than control group (p<0.05). However, no significant differences were observed between the plasma high-density lipoprotein cholesterol levels of GRGT and other groups.
In fifth part, the effect of GRGT on body weight loss and the improvement of plasma lipid level of male person(40$\sim$50 ages) were also investigated. The overweighted male (BMI>25) were drunk into the beverage (560 mL, 5.6 g tea dr. wt. equivalent) of GRGT for four weeks. The levels of triglyceride and plasma total-cholesterol were reduced by green teas but there was no difference between GRGT and GT groups.
This results indicated that GRGT might exert potent anti-obesity and the improvement of plasma lipid level in diet-induced obese rats.
The sixth part was evaluated the major components and bioactivity in both gaba tea and green tea. Total phenolics, ascorbic acid and chlorophyll were lower in gaba tea than those of green tea. However, there were no differences in water content, total nitrogen, tannin, caffeine and reducing sugar contents. In amino acids, aspartic acid and glutamic acid were much lower in gaba tea than those of green tea. Epicatechin and epigallocatechin contents were significantly decreased in gaba tea compared to green tea. Among minerals, Mg content is lower in gaba tea than green tea. In bioactivities, ACE inhibition activity is much higher in gaba tea than those of green tea.
The seventh part was conducted to determine the shelf life of gaba tea during storage. Sensory quality and visual score decreased with time in both cold and ambient temperature storage. Catechin content and sensory quality were closely related PPO activity during storage. Shelf life of gaba tea in cold and ambient temperature storage were 6 and 8 months, respectively In packing methods, shelf life considerably reduced when the tea bag open or clipped with silica gel, regardless of storage condition.
The eighth part was conducted to produce gaba tea and investigate consumer panel test. Gaba tea was produced by roasted processing, In economic analysis, gaba tea farmer earned much money about 174,000won/10a compared to green tea. In nutrition facts, gaba tea has 0 calories, 0 mg Na, 0 mg fat, 3.8 mg total phenolics, 8.6 mg gaba content per g dry weight. For consumer panel test with 200 people from the big city, they liked gaba tea products with increasing good taste and functional nutrients such as gaba, ascorbic acid and catechins. The optimal retail consumer price was 30,000 won per gaba tea product(50g).

목차 Contents

• 표지 ... 1
• 제출문 ... 3
• 요약문 ... 5
• Summary ... 11
• Contents ... 15
• 목차 ... 18
• 제 1 장 서론 ... 21
• 제 1 절 연구개발과제의 개요 ... 21
• 1. 연구의 목적 ... 21
• 2. 연구 내용과 범위 ... 21
• 제 2 절 국내외 기술개발 현황 ... 22
• 1. 국내에서 기능성 차 개발 ... 22
• 2. 해외에서 기능성 차 개발 ... 25
• 제 2 장 다원에서 광 파장 및 신호전달물질 살포에 의한 가바 증진 ... 26
• 제 1 절 서론 ... 26
• 제 2 절 재료 및 방법 ... 26
• 제 3 절 실험 결과 및 고찰 ... 30
• 1. 광파장 처리 효과 ... 30
• 2. 신호전달물질 처리 효과 ... 39
• 3. 자외선과 신호전달물질 살포 효과 ... 49
• 제 3 장 제다전 혐기처리에 의한 가바증진 ... 54
• 제 1 절 서론 ... 54
• 제 2 절 재료 및 방법 ... 54
• 제 3 절 실험 결과 및 고찰 ... 55
• 1. 냉수침지 처리 효과 ... 55
• 2. 유산균주를 이용한 가바증진 ... 58
• 3. 질소 혐기처리 효과 ... 60
• 제 4 장 고지방 식이를 섭취한 흰쥐에서 가바녹차의 항비만 효과 ... 71
• 제 1 절 서론 ... 71
• 제 2 절 실험재료 및 방법 ... 72
• 제 3 절 결과 및 고찰 ... 76
• 제 5 장 혐기 처리에 의해 제조된 가바녹차의 기능 성분 증대 및 생리활성 ... 88
• 제 1 절 서론 ... 88
• 제 2 절 재료 및 방법 ... 90
• 제 3 절 결과 및 고찰 ... 94
• 제 6 장 성인남자에서 가바녹차 섭취가 비만 억제 및 콜레스테롤 감소 효과 ... 100
• 제 1 절 서론 ... 100
• 제 2 절 실험재료 및 방법 ... 100
• 제 3 절 결과 및 고찰 ... 103
• 제 7 장 가바차 주요 특성 ... 121
• 제 1 절 서론 ... 121
• 제 2 절 재료 및 방법 ... 121
• 제 3 절 실험 결과 및 고찰 ... 125
• 1. 가바차에서 주요특성 ... 125
• 2. 차 추출물에서 주요 특성 ... 129
• 제 8 장 가바차 유통중 품질 변화 ... 135
• 제 1 절 서론 ... 135
• 제 2 절 재료 및 방법 ... 135
• 제 3 절 실험 결과 ... 135
• 제 9 장 가바차 시제품 생산 및 유통 ... 141
• 제 1 절 시제품 생산 공정 개발 ... 141
• 제 2 절 시제품 생산 ... 143
• 제 3 절 경제성분석 ... 145
• 제 4 절 시제품 개발 설명회 및 소비자기호도 ... 147
• 참고자료 및 인용 문헌 ... 150
• 끝페이지 ... 156