초록

○ 연구결과
상황버섯의 속성재배 환경을 확립하였으며 동충하초의 유효성분의 안정적인 유지방법과 양호한 자실체 생성의 기술을 확립하였다. 신령버섯 자실체의 맛과 향을 개량하고 유효성분을 증대시키는 배지를 개발을 성공하였으며, 나아가 신령버섯의 괴균병 억제물질을 탐색하여 방제약품을 개발하였다.
이들 약용버섯의 약효성분을 분석하고 약효검정을 in vitro 세포배양을 통하여 실시함과 동시에 항암, 면역증강기전을 규명하였다. 뿐만아니라 이들 약용성분을 이용한 산업화 방법을 모색하고 상품화를 꾀하였다.

Abstract ▼

IV. Results and Discussion
Medicinal mushrooms such as Phellinus, Agaricus, and Cordyceps are approved as food and many farmers are cultivating these mushrooms. Although the mushrooms are valuable farmers are in financial trouble because there are cultivation difficulties.
For Phellinus linteu

IV. Results and Discussion
Medicinal mushrooms such as Phellinus, Agaricus, and Cordyceps are approved as food and many farmers are cultivating these mushrooms. Although the mushrooms are valuable farmers are in financial trouble because there are cultivation difficulties.
For Phellinus linteus it takes too long cultivation period, contamination frequency was high, and no scientific analysis and laboratory data supported food products. All of these explain export troubles.
In case of Cordyceps militaris, normal fruit body were not produced due to high mutation frequency. Previously each subculture was carried out with a piece of tissue from ascocarp. However, tissue culture also accompanied with contamination.
In case of Agaricus blazei it has been excluded due to bad flavor and taste on the consumer's dining table although it has been know to have anticancer function. Agaricus farmers face financial problem due to over 30% damage by False truffle disease.
The followings are our accomplishment to solve the problem mentioned above:
(1) We developed new media and culture condition to reduce cultivation period for Phellinus linteus, which is difficult to culture, and transferred the developed technique to farmers. We also obtained research data which will help export.
(2) Two of liquid culture media were developed and made as commercial product in agreement with company obtaining good quality reputation.
The commercial product can be used for other mushroom culture media, which will be helpful for mushroom farmers and companies.
(3) New media for Agaricus blazei were developed to improved flavor and taste.
The amount of beta-glucan (36.1% increment) was increased to 14.7% from 10.8% of rice straw substrate
(4) We solved major hinderance, False truffle disease, for Agaricus blazei cultivation. The discovered compounds are parts of chemical fertilizer which is cheap and nontoxic and gives fundamental control means.
(5) We developed a stable subculture technique for Cordyceps militaris preventing mutation. It was discovered that spores produced during liquid culture was related with mutation. Removing spores from liquid culture produced normal ascocarp after sequential subcultures. This way accelerates production of normal ascocarp and activates food process industry of Cordyceps militaris.
Optimum extraction method for Phellinus linteus was developed. Extraction was carried out with 100 time of water to Phellinus linteus fruit body by heating under high pressure. Bean-size of fruit body was the best size to obtain high yield extraction and filtration. Purification of beta-glucan started with concentration followed by 3 time volume of ethanol precipitation. More than 70% pure beta-glucan was obtained and used for commercial powder product.
(6) Functional compound of medical mushrooms was investigated for anticancer activity and immune response boost. The resulting data can be used to export the mushrooms.
(7) Development of food containing medical mushrooms.
Cordyceps militaris extract product was transferred to a related company and has been sold for a year and salt products containing Cordyceps militaris or Phellinus linteus were also developed.
Purification of Phellinus beta-glucan was accomplished and license contract will be made with a proper company.
The above results have been presented at symposiums with handout and provide solid platform to activate mushroom industry.
Suggestion for application of research results at a government-level:
There is no government-approved analysis Institute or Research center for functional compounds from mushrooms. The Institute can analyze functional compounds fast at low service price. Currently laboratories of universities conduct analysis. When a technician graduates analysis becomes difficult. Service charge costs 300,000-400,000won/analysis when analysis was done in Japan. A kit of "Megazyme analysis" costs about 400,000won and can be used for 100 times analysis. Therefore, tenured-skillful technician is a key factor for development of mushroom industry.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 12
• CONTENTS ... 20
• 목 차 ... 23
• 제1장. 연구 개발 과제의 개요 ... 25
• 제1절. 연구 개발의 필요성 ... 25
• 1. 기술적 측면 ... 26
• 2. 경제 . 산업적 측면 ... 28
• 3. 사회 . 문화적 측면 ... 28
• 제2절. 연구 개발의 범위 ... 30
• 제2장. 국내외 관련기술의 현황과 문제점 ... 31
• 제1절. 국내 관련 기술의 동향 ... 31
• 제2절. 국외관련기술의 현황 ... 34
• 제3장. 연구 수행 내용 및 결과 ... 35
• 제1절. 재료 및 방법 ... 35
• 1. 약용버섯(상황)의 속성재배를 위한 최적 배지와 재배 환경 확립 ... 35
• 2. 약용버섯(동충하초)의 안정적인 계대 배양을 통한 정상적인 자실체 형태 유지 방법 개발 ... 37
• 3. 약용버섯(신령버섯)의 맛 향상과 유효성분을 증대시키는 배지 개발 ... 38
• 4. 신령버섯의 괴균병 방제 약품의 개발 ... 39
• 5. 약용버섯 속성 재배용 액체종균의 개발 ... 40
• 6. 인공재배 약용버섯(상황) 자실체로부터 유효물질의 최적 분리, 정제 기술 개발 ... 41
• 7. 약용버섯 유효물질의 항암효과 검색과 항암 작용 규명 ... 43
• 8. 상황버섯 다당체의 수지상세포 활성에 미치는 영향 ... 50
• 9. 신령버섯 다당체와 동충하초 다당체의 면역활성능 검증 ... 52
• 10. 약용버섯 성분 함유 식품의 개발 ... 53
• 제2절. 연구 결과 및 고찰 ... 54
• 1. 약용버섯(상황)의 속성재배를 위한 최적 배지와 재배 환경 확립 ... 54
• 2. 약용버섯(동충하초)의 안정적인 계대 배양을 통한 정상적인 자실체 형태 유지 방법 개발 ... 70
• 3. 약용버섯(신령버섯)의 맛 향상과 유효성분을 증대시키는 배지 개발 ... 72
• 4. 신령버섯의 괴균병 방제 약품의 개발 ... 74
• 5. 약용버섯 속성 재배용 액체종균의 개발 ... 77
• 6. 인공재배 약용버섯(상황) 자실체로부터 유효물질의 최적 분리, 정제 기술 개발 ... 82
• 7. 약용버섯 유효물질의 항암효과 검색과 항암 작용 규명 ... 91
• 8. 상황버섯 다당체의 수지상세포 활성에 미치는 영향 ... 120
• 9. 신령버섯 다당체와 동충하초 다당체의 면역활성능 검증 ... 127
• 10. 약용버섯 성분 함유 식품의 개발 ... 131
• 11. 속성 재배 기술 요약 및 관행재배법에서의 개선점 ... 133
• 12. 약효 증대에 대한 추가 실험 결과 ... 136
• 참고문헌 ... 140
• 끝페이지 ... 143