초록 ▼

○ 연구결과: 당근 저장 시 부패 미생물의 순수 분리 및 균주 동정, 분리된 부패 미생물에 대한 항균세척제의 억제 활성 조사, 당근에 적합한 chitosan 코팅제의 제조 최적 기술 확립, 그리고 당근에 적합한 항균성 코팅 피막제를 개발하였다. 당근의 수확 후 생리적, 병리적 특성, 당근의 장기저장에 적합한 적정 저장온도와 습도 조건, 당근에 적합한 가스 환경 구명과 적정 MA조건 등을 구명하였고, 당근의 장기저장에 적합한 적정 저장온도와 습도 조건 실증 적용, 당근 유통에 적합한 소포장 기술, 항균성 코팅피막제 처리기술, 항균성

○ 연구결과: 당근 저장 시 부패 미생물의 순수 분리 및 균주 동정, 분리된 부패 미생물에 대한 항균세척제의 억제 활성 조사, 당근에 적합한 chitosan 코팅제의 제조 최적 기술 확립, 그리고 당근에 적합한 항균성 코팅 피막제를 개발하였다. 당근의 수확 후 생리적, 병리적 특성, 당근의 장기저장에 적합한 적정 저장온도와 습도 조건, 당근에 적합한 가스 환경 구명과 적정 MA조건 등을 구명하였고, 당근의 장기저장에 적합한 적정 저장온도와 습도 조건 실증 적용, 당근 유통에 적합한 소포장 기술, 항균성 코팅피막제 처리기술, 항균성 세척처리 기술 등을 개발하였다.

Abstract ▼

This study was carried out to develop the washing and packaging technology available effectively for maintaining the high quality and a long-term storage to minimize the postharvest losses of carrot. The summarized results were as follows;
○ Ethylene evolution was not detected in washed carrot du

This study was carried out to develop the washing and packaging technology available effectively for maintaining the high quality and a long-term storage to minimize the postharvest losses of carrot. The summarized results were as follows;
○ Ethylene evolution was not detected in washed carrot during cold storage. Respiration rate was higher found in not-packaged carrot while its lowest value was produced in packaged carot with LDPE film(30μm).
○ The peel a value was not greatly changed during storage of raw carrot before washing, There was an increasing trend of the pulp pH value in stored carrot packaged with different kinds of film and their thickness.
○ Sprouting and rooting were found in carrot after 60 cold storage days, and the market quality was better maintained in packaged carrot with LDPE film than samples with PEPP due to the increasing decay incidence.
○ For a long-term storage, we concluded that the best storage environment of carrot should contain the range of temperature at -0.5oC～＋0.5oC and the relative humidity at least more than 95% r.H.
○ The active MA( 5% CO₂ + 3% O₂ ) has led to the good result of holding the market quality with the packaging unit of 500g packages compared to 1000g sizes independent upon packaging materials.
○ Carrot lost most its weight in case of washing with tap water while 2% thiabendzole in 80% DMF and 5% sodium hypochlorite showed the better maintaining effect of the initial weight. And two compounds of 2% thiabendazole and 5% sodium hypochlorite showed better fungicide effects on maintaining external market quality of carrot.
○ Coating material made with kitosan molecular weight of 50CPS was the best effective for retaining overall quality of stored carrot.
○ In a second research term, the firmness, SSC and pH value were a little well maintained in an active MA condition of 10% CO₂ + 3% O₂ combination compared to the 5% CO₂ + 3% O₂ that was reported as the best condition among all gas treatments in 2004. But their difference of effectiveness was not statistically accepted.
○ Considering on peel drying, sprouting, rooting and decay incidence, the active MA condition of 5% CO₂ + 3% O₂ combination showed the best market quality in packaged carrot with 0.05mm LDPE film.
○ Acetaldehyde and ethanol contents were the most investigated in a not-packaged carrot and in a active MA( 10% CO₂ +3% O₂) packaged with 0.05mm BOPP film, and their results showed same analytical trends with overall external quality evaluation in stored carrot.
○ Two compounds of A(ultrasonicated kitosan 2% in 0.5% acetic acid + 0.5% potassium sorbate + 0.5% lauric acid + 10% Triton X-100) and B(ultrasonicated kitosan 2% in 0.5% acetic acid +0.5% potassium sorbate + 1.0% lauric acid + 10% Triton X-100) represented the best result of holding the overall market quality related with drying, decay, peel color.
○ In comparison of chitosan-laurate film depending upon different concentrations of chitosan and lauric acid, two treatments of A (chitosan 2% in 0.5% acetic acid + 0.5% potassium sorbate + 0.5% lauric acid + 10% Triton X-100) and B (chitosan 2% in 0.5% acetic acid + 1.0% lauric acid +10% Triton X-100) maintained low contents of acetaldehyde and ethanol throuthout the cold storage of carrot.
○ After results from Songsan Nonghyup in 2004, the pallet covering with 0.03 LDPE film and PEPP film represented no evident peel drying and decay incidence compared to the carrot in not-covered pallet. And sprouting and rooting were greatly inhibited by 0.03mm LDPE packing while they were rather promoted in PEPP packing due to the high humidity inside in both of washed and raw carrot.
○ After results from Songsan Nonghyup in 2005, 5% sodium hypochlorite showed the marked inhibition of decay incidence except physiological processes such as sprouting and rooting. It could be concluded that another treatment together with 5% sodium hypochlorite be used to keep carrot fresh for long-term storage because of sprouting and rooting.
○ To identify the bacterium Ca-1, which was isolated from th decayed carrot, chromosomal DNA purified from Ca-1 was amplified with 16srDNA primers by PCR. Ca-1 was identified as Erwinia carotovora from the result of sequence homology of the amplified DNA fragment. This result was confirmed by the physiological and biochemical tests and electron microscopy.
○ Ca-2 and Ca-s-3 isolated from decayed carrot were identified by the morphological, physiological and chemotaxonomical characteristics as Rhyzophus oryzae and phomopsis mali, respectively.
○ Antimicrobial activity of thiabendazole, sodium hypochlorite and calcium hypochlorite, potassium phosphate, and potassium hydrogen phosphate against Ca-1, Ca-2 and Ca-s-3 were determined by agar diffusion assay. Thiabendazole and sodium hypochlorite were most effective against Ca-1, ca-2 and Ca-s-3.
○ Total number of microorganism and the spore number of fungi were decreased in the carrots, which were dipped in 2% thiabendazole solution for 10 seconds in compared to control carrots, which were not treated with that solution.
○ The best conditions for ultrasonication of chitosan were 2h ultrasonicaton of 250 ml of 2% chitosan solution.
○ Viscosity and molecular weight of ultrasonicated 2% chitosan were 57 CPS and 50,000, respectively.
○ Antimicrobial activities against Ca-1, Ca-2 and Ca-s-3 were determined for 2% ultrasonicated chitosan solution added with thiabendazole, sodium hypochlorite and potassium sorbate in different concentrations. 2% ultrasonicated chitosan solution added with 0.5% potassium sorbate was most effective on the growth inhibition of Ca-1, Ca-2 and Ca-s-3.
○ Antimicrobial activities against Ca-1, Ca-2 and Ca-s-3 were determined for 2% ultrasonicated chitosan solution added with PEG 400. 2% ultrasonicated chitosan solution added with PEG 400 in the ratio of 4 : 1 was most effective on the growth inhibition of Ca-1. In case of Ca-2 and Ca-s-3, 2% ultrasonicated chitosan solution added with PEG 400 in the ration of 2 : 1 was most effective.
○ Antimicrobial activities against Ca-1, Ca-2 and Ca-s-3 were determined for 2% ultrasonicated chitosan solution added with lauric acid and potassium sorbate in different concentrations. 2% ultrasonicated chitosan solution added with 1% lauric acid and 0.5% potassium sorbate was most effective on the growth inhibition of Ca-1, Ca-2 and Ca-s-3.
○ It was investigated about antimicrobial activity of chitosan monomer, glucosamine against Ca-2. The growth of Ca-2 on the PDA added with glucosamine was not inhibited in compared to that on the PDA without glucosamine.
○ Morphological character of Ca-2 treated with ultrasonicated chitosan added with potassium sorbate was examined by electron microscopy. Swelling of spore and irreglular morphological character were examined.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 9
• CONTENTS ... 13
• 목차 ... 14
• 제1장 연구개발과제의 개요 ... 15
• 제1절 연구개발의 목적 및 필요성 ... 15
• 제2절 연구개발의 범위 ... 17
• 제2장 국내외 기술개발 현황 ... 18
• 제1절 국내기술현황 ... 18
• 제2절 국외기술현황 ... 18
• 제3절 국내기술의 취약성 ... 19
• 제3장 연구개발 수행내용 및 결과 ... 20
• 제1절 당근의 장기저장 기술과 세척 및 포장기술 개발 ... 20
• 1. 서론 ... 20
• 2. 재료 및 방법 ... 20
• 가. 시료 및 실험처리 ... 20
• 3. 결과 및 고찰 ... 24
• 가. 당근의 수확 후 생리적, 병리적 특성 및 적정 저장온도와 습도조건 구명 ... 24
• 나. 당근에 적합한 소포장 및 적정 MA 조건 구명 ... 27
• 다. 당근의 항균성 세척처리 및 코팅피막제 처리기술 개발 ... 40
• 라. 선발된 당근 포장지별 최적 active MA 조건 구명 ... 43
• 마. 분자량별 chitosan 및 lauric acid 농도 처리별 당근의 저장력 조사 ... 48
• 바. 당근의 장기저장을 위한 현지 실증연구(성산농협) ... 51
• 제2절 당근의 장기저장, 유통에 적합한 항균성 피막 코팅제의 개발<위탁과제: 순천향대학교> ... 55
• 1. 서론 ... 55
• 2. 재료 및 방법 ... 55
• 3. 결과 및 고찰 ... 60
• 가. 당근 저장 시 부패 미생물의 순수 분리 및 균주 동정 ... 60
• 나. 분리된 부패 미생물에 대한 항균세척제의 억제 활성 조사 ... 63
• 다. 분자량별 chitosan 코팅제의 제조 및 향균력 조사 ... 65
• 라. 당근에 적합한 chitosan 코팅제의 제조기술 개발 ... 70
• 마. Chitosan-laurate의 합성 및 제막 및 항균할성 조사 ... 73
• 제4장 목표달성도 및 관련분야에의 기여도 ... 77
• 제5장 연구개발결과의 활용계획 ... 78
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 79
• 제7장 참고문헌 ... 80
• 끝페이지 ... 82