초록

○ 연구결과
-조사료용 다수확 품종개발: 국내 유전자원을 이용한 조사료용 옥수수 개발 결과 생체 46%, 건물 37% 및 이삭중 15%가 중수되었으며, 도복에 유리하고 , 후기녹체성에서도 크게 개선됨.
-분얼형 옥수수의 특이적 분자마커개발: 다얼성에서 알려진 후기녹체성 부진 및 착수고가 높은 단점을 보완할 수 있는 stay green gene 과 low ear height인자를 분리, 동정 후 이들에 대한 분자 표지마커가 개발 가능할 것으로 판단됨.

Abstract ▼

This study was carried out to develop the crude forage maize hybrid with high yielding and tolerance to environmental stresses.
Materials used in this study were multi-tiller and ears lines, low-eared lines semi-dwarf lines and stay-green lines. These four groups were receprocally crossed for m

This study was carried out to develop the crude forage maize hybrid with high yielding and tolerance to environmental stresses.
Materials used in this study were multi-tiller and ears lines, low-eared lines semi-dwarf lines and stay-green lines. These four groups were receprocally crossed for making the F₁ hybrids at Daejeon in 2001.
About 120 hybrids including check hybrid, Kwanganok were planted at three regions; Daejeon (May 15), Suwon (May 5) and Milyaing (May 20) in 2002, and these hybrids were evaluated for stem height, ear height, stem diameter and days to tasseling for biological characters. Fresh weight and dry matter were measured for selection of superior crude forage hybrid.
Degree of lodging and disease-pest injury were also scored for selection the tolerance hybrids to environmental stresses and stay-green degrees to nutritional values.Heriterbility, heterosis correlation coefficients and combining ability about crossing parents were evalutated to increse the efficiency of selection for the superior hybrid corn breeding.
Results obtained from this experiment were as follows:
Average percent of heterosis for the stem height, ear height, ear diameter and 100 kernel weight showed a high positive values, while tiller number per plant was negative.
Characteristics measured were highly significant in both general and specific combining ability. GCA effects of the stem height and ear height were highly significant in hybrids crossed with CNU. SCA effects of stem height and ear height were highly significant in all crosses, while showed negative values of SCA.
Among hybrids, six hybrid selected in 2002 and planted in 2003. Hybrid CNU2618${\times}$ CNU2665, CNU2627${\times}$CNU3007 and CNU2618${\times}$CNU2680 were higher than Kwanganok check in stem height, but lower in ear height. Ratio of ear height to stem height was similar or lower than check.
Therefore, cultivation of these hybrids will be useful to environmental stress such as lodging. Especially, hybrids CNU2627${\times}$CNU3007 and CNU30l4${\times}$CNU2678 were higher than check hybrids in both fresh and dry weight because of multi-tillers per plant, and grain yield was also higher than check. Hybrid CNU2618${\times}$CNU2665, CNU3054${\times}$SK1 and CNU2798${\times}$SK1 possessing no-tiller were higher than check in both fresh and dry weight yield per 10a. Degree of Insect and disease injuries such as leaf slight and leaf spot except corn borer were similar to check. Stay green degree of Hybrids crossed with lines having stay green gene were similar to check hybrid. In the whole experiment, hybrid CNU2618${\times}$CNU2665, CNU3054${\times}$SKl, CNU2798${\times}$SK1 were better than check for new crude forage use, but some supplements before practical cultivation were indicated.
Accordingly, these hybrids have to be evaluated in both a proper planting density and fertilizer or compost levels and must have two to three years for production performance test and local adaptation test before relase to the farmers.Development of Molecular markers in maize with Multi-tiller and EarIn order to elucidate molecular events on the formation of multi-tillers in maize, we conducted EST analysis and differential hybridization (DH) at the early development of tillers. Total 118 clones were sequenced and produced some length of readable sequences. ESTs were divided into 11 functional categories.Cell division and growth related genes represented 4 clones, including growth hormonel protein, leaf development protein Argonaute, MADS box-like protein, and plastid division protein FtsZ. Two genes, ZmRPS4 and ZmMET1, were isolated by DH methods and characterized by Nothern blotting and in situ hybridization. Both of ZmRPS4 and ZmMET1 were expressed in vascular tissues of the crown of tiller. Especially, the ZmMET1 gene were highly expressed in leaf primordium of the crown of tillers. These genes were also induced by exogenous treatment of cytokinin in the leaf.AFLP analysis using 64 primer pairs were conducted in order to identify stay green multi ear and tiller (MET) type maizes. Two primer combinations identified three fragments and sequenced with an aim towards converting AFLP markers into sequence tagged sites (STSs). Primer set, termed MET2, amplified a stay green specific fragment. In AFLP analysis on low hight ear maize, five primer combinations produced nine specific polymorphic fragments.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 7
• CONTENTS ... 10
• 목 차 ... 12
• 제 1 장 연구개발 과제의 개요 ... 14
• 제 1 절 연구개발의 필요성 ... 14
• 제 2 절 연구개발의 목표 및 내용 ... 19
• 제 3 절 연구개발 목적 및 범위 ... 21
• 제 4 절 연구개발 방법 및 실제 ... 23
• 제 2 장 국내ㆍ외 기술개발 현황 ... 26
• 제 1 절 국내ㆍ외 기술현황과 문제점 ... 26
• 제 2 절 앞으로의 전망 ... 29
• 제 3 절 기술도입의 타당성 ... 30
• 제 3 장 연구개발 수행내용 및 결과 ... 31
• 제 1 절 안정ㆍ다수성 우수 교잡종 육성 ... 31
• 제 2 절 다수다얼성(MET) 특이적 유전자의 분리 및 동정 ... 56
• 제 3 절 후기 녹체성 및 저착수고 선발을 위한 분자마커 개발 ... 69
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 78
• 제 5 장 연구개발 결과의 활용계획 ... 80
• 제 6 장 연구개발 과정에서 수집한 해외 과학 기술 정보 ... 82
• 제 7 장 참고문헌 ... 84
• 끝페이지 ... 90