초록 ▼

○ 연구결과
두릅나무의 추출물을 제조하여 일반성분 분석 및 TLC, HPLC 성분 분석을 통하여 품질관리 기준을 연구한 결과 HPLC의 phenolic 성분의 분석에 따라 3,4-dihydroxy benzoic acid의 함량을 기준으로 하여 품질관리가 가능함을 제시하였다. 두릅나무 추출물에서 새로운 기능성 개발 연구에서는 두릅 추출물에서 항산화력이 높은 ascorbate(Vit C) 수준의 항산화력이 관찰되었고 당 축적시 polyol 생성을 촉진하는 효소 aldose reductase의 활성을 억제하는 효능을 관찰하였으며,

○ 연구결과
두릅나무의 추출물을 제조하여 일반성분 분석 및 TLC, HPLC 성분 분석을 통하여 품질관리 기준을 연구한 결과 HPLC의 phenolic 성분의 분석에 따라 3,4-dihydroxy benzoic acid의 함량을 기준으로 하여 품질관리가 가능함을 제시하였다. 두릅나무 추출물에서 새로운 기능성 개발 연구에서는 두릅 추출물에서 항산화력이 높은 ascorbate(Vit C) 수준의 항산화력이 관찰되었고 당 축적시 polyol 생성을 촉진하는 효소 aldose reductase의 활성을 억제하는 효능을 관찰하였으며, 렌즈배양시 백태 생성을 25% 억제, 당뇨유발랫드에서도 유의적인으로 백내장 생성 억제하였다. 또한 급성독성시험에서 두릅 추출물 2000mg/kg 경구투여하였을 때 전혀 독성이 관찰되지 않았으므로 안전하게 사용될 수 있는 식품 원료임을 입증하였다. 품질관리 연구와 기능성 연구의 결과에 따라 건강보조 식품의 수요대상을 당뇨합병증 또는 당뇨성 백내장으로 하고 제품의 concept 및 조성을 결정하였고 이에 따라 pilot 규모의 산업적 추출방법을 확립하였고 제형 연구를 통해 타정 및 PTP 포장을 선정하고 시제품을 생산하였다.
시장 조사에서는 아직 성공적인 당뇨합병증 예방 상품이 없기 때문에 상당규모의 틈새 시장이 확보되어 있으므로 본 연구 결과에 따라 생산 될 제품의 신장진입이 기대된다. 기출원한 국내 특허가 2002년에 등록되었고 2002년 하반기에는 미국특허를 출원하였으며, 최근 국내 특허의 실시권을 판매회사에 이전한 바 있다.
본 연구는 두릅나무의 작물 재배와 연계하여 수행되었다. 특용작물의 대량 생산을 유도하여 농가의 소득을 증대시키기 위하여 보급․재배하기에 적합한 우수품종 및 대량생산기법을 개발하였다. 나무 및 가지의 생육 및 특성조사, 병해검정을 통해 수세가 양호하고 입고역병과 더뎅이병에 내병성이며 재배적으로 유리한 형질을 보유한 성목 4계통, 유목 4계통 6개체 등 총 10개체를 선발하여 근맹아 번식에 의한 후대번식을 실시하였고, 종자 전처리 및 저장방법 개발로 안정적인 발아 조건을 확립하고 근삽에 의한 번식기술을 개발하여 대량증식기술을 확립하였다. 촉성재배를 위한 정아삽 재배와 식재방법별 재식적기, 식재종류별 적정 재식방법 등의 재배기술을 개발하여 대량생산기술의 기반을 마련하였다.

Abstract ▼

Ⅳ. Study results and application
1. Development of the method for quality control and of the nutraceutical products of the extract from Aralia elata
◦ The extracts were prepared in bench scale from the different raw materials; different parts of the plants or different species and analyzed in

Ⅳ. Study results and application
1. Development of the method for quality control and of the nutraceutical products of the extract from Aralia elata
◦ The extracts were prepared in bench scale from the different raw materials; different parts of the plants or different species and analyzed in general nutrients and heavy metals. In results, the differences in general nutrients the were found among the sprout, the branches and the roots and the amounts of heavy metals including Pb and Cr were in the safe ranges, much less than maximum recommendation.
◦ Chemical analysis of the extracts revealed that phenolic compounds and saponins were major components in their quantity and the major phenolic compounds were chlorogenic acid(CLA), caffeic acid(CFA) and 3,4-dihydro-benzoic acid(DHBA).
◦ The amounts of phenolic compounds in the branches showed higher than those in the roots or new branches. There was no significant difference in the quantities of phenolic compounds between the species with or without the thorns. However, the branches collected in the spring showed the lower levels of phenolic compounds compared with those in the fall. The amount of DHBA in the fall was 2 times higher than that in the spring. The older age of the plant contained the more amount of DHBA. And the analysis of six species based on the disease tolerance and on the growth condition demonstrated that there were no significant differences in phenolic compounds except one species and the levels were comparable with those of naturally grown plants. Thus, it may be suggested that the best raw materials for the extract and health food could be the branches, the two years grown, and with disease tolerance.
◦ DHBA was conducted as a component for quality control on the bases of the results from the TLC and HPLC analysis in the relationship with the bio-functions. It was concluded that the quantity of DHBA should be over 1.10% to maintain the good quality of the products.
◦ From the repeated analysis of the various extract of Aralia elata, it was verified that the extraction method in bench scales were well established.
◦ From determing the inhibitory effects on the aldose reductase activity and the antioxidant activity, it was demonstrated that the antioxidant activity of the extract were prominent as that of ascorbate and AR inhibitory activity were dominant. Only the extract from raw materials collected in the spring represented the low anti-oxidant activity. This result seems to be accordant to the analytical result of phenolic components. Thus the spring collected material may be not proper to the health food.
◦ Anti-cataract effects of the extracts were confirmed using the lens culture system.
In addition, when the extract was administered to the STZ-induced diabetic rats for 10 weeks, the cataract formation was inhibited in some how. Thus, the same conclusions in the bio-functions were made from in vitro, ex vivo, and animal experiments. Thus, the scientific and technical bases for the bio-functions might be enough to entering the market for product and promising the successful manufacturing and marketing.
◦ The fractions extracted with organic solvents were chemically anaylzed and examined of their bio-functions. Then, it was found that the chloroform and ethy acetate fraction had prominent antioxidant activity and inhibitory activity of AR compared with butanol fraction and water soluble residue. From the lens culture experiments, the chloroform fraction showed more effective on the inhibition of cataract formation. The effective chloroform fraction was applied to the Silica gel chromatograph and the collected fractions showed the inhibitory effect on the cataract in the dose dependent manner.
◦ From the searches on the size and characteristics of markets, it was realized that the population of the elderly and the incidences of diabetes and cataract increase every year, but the effective medicine or the health food against diabetes complications have not been well developed yet. Thus, a large niche market has been formed for the venture company.
◦ In order to establish the proper manufacturing process the extaction method in bench scale were applied to the pilot scale. Then, the production yield of th extract were 7% in both scales.
◦ When a single dose toxicity test was performed to the rats(female and male), the LD50 were estimated over 2000 mg/kg body weight. It was concluded that the extract was very safe to the life in terms of toxicity.
◦ Based on the above results, the extract could constitute as a major component of the food supplement(EyePro) or special nutrient supplement(DiaPro). The remaining components were formulated on according to the product concept.
◦ When DiaPro were administered to the STZ-induced diabetic rats for 12 days, the blood glucose levels with the oral force feeding of DiaPro at the dose of 600 mg/kg decreased to 32% compared to the diabetic rats.
◦ As a product of this study, patent related to Aralia elata were registrated in Korea and the right of patent practice have been successfully transferred to GYintec Co., Ltd.
It is expected that the manufacturing products of health food introduce to the market in the next season of the year.
2. Breeding and development of cultivation techniques for mass propagation of Aralia elata SEEM.
◦ We had collected 87 lines of Aralia elata SEEM., mature trees and seeds, all over the country. After testing of specific characteristic, 200 individuals which have high quality characteristic were selected by primary selection. We have preserved them as germplasm and used them as testing materials in variety selection in 2002.
◦ 4 individuals of mature tree and 6 individuals of young tree through 4 lines which have vigorous tree and disease tolerance to Phytophora cactorum and Elsinoe araliae were selected with results of examination about growth condition and specific characteristic on trunk and branch.
◦ For seedling in fall, seed coat were immediately removed after seed harvesting.
Seedling in the greenhouse after chilling treatment (4℃, 2 weeks) caused 31% increasing of sprouting ratio than conventional seedling. That result can makes steady seedling in winter.
◦ For seedling in spring, seeds mixed with wet sands(seeds:sands=1:3) represented 85.7% sprouting ratio after low temperature : there is no sprouting after room temperature.
◦ For propagation with roots, 10∼15㎜ thickness roots were gathered and cut as 15㎝ in length. After storage under soil, seedling with A-type plug represented best efficiency per area at early spring.
◦ For rapid mass propagation after selection, propagation by asexual propagation with root sprouting(slightly low propagation efficiency) is more proper than sexual propagation with seedling (high propagation efficiency, but cannot fix superior characteristics gained by breeding)
◦ Forcing culture in winter had more advantage because of early shipment than regular shipment. Apical bud cutting represented so high quality that its price is 18% higher than others.
◦ Forcing culture after apical bud cutting represented best result at the condition of over 2㎝ thickness and 70㎝ in length. Treatment of GA3 50 ppm at apical and lateral bud can makes early harvest(about 11 days) than control.
◦ For outdoor culture, the capping at tip with transparent or opaque vinyl tube(6㎝×20㎝) makes about a week early harvest than control but have low quality and preference because of its flabby tissue and yellow color.
◦ For cultivation on mountainous district, planting seedlings rapidly make a stock but need high cost for seedlings, labors, and management. Root cutting is more proper because of bad condition to work as slant.
◦ For cultivation on mountainous district, root cutting represented 67% survival rate when 1㎝ thickness and 15㎝ in length root from 2-year-old young tree at fall was planted at the sunny spot.
◦ Because cultivars of Aralia elata SEEM. can be protected by 'Seed Industry Law' from 2007, selected superior lines after breeding will propagate and test yield from 2003 to 2004, test regional adaptation apply for a patent from 2005 to 2007, supply seedlings for the farmers, share germplasm with the breeders, make the best use of developed method of mass propagation and cultivation as farming apply materials.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 9
• CONTENTS ... 16
• 목차 ... 17
• 제1장 연구개발과제의 개요 ... 18
• 제1절 연구개발의 목적 및 필요성 ... 18
• 제2절 연구개발의 내용 및 범위 ... 22
• 제2장 국내외 기술개발 현황 ... 23
• 제1절 두릅나무 기능성 및 기능 성분에 관한 연구 ... 23
• 제2절 당뇨성 백내장 연구와 치료 물질 소재 및 제품 ... 24
• 제3절 두릅의 신품종 육성 및 대량증식 재배기술 개발 ... 28
• 제3장 연구개발수행 내용 및 결과 ... 31
• 제1절 두릅 추출물의 품질관리 및 제품화 연구 ... 31
• 1. 두릅 추출물의 성분 분석 및 품질관리 ... 31
• 2. 두릅 추출물의 기능성 연구 ... 43
• 3. 두릅 추출물의 제품화 연구 ... 55
• 제2절 두릅의 신품종 육성 및 대량증식 재배기술 개발 ... 93
• 1. 유전자인 수집 및 우량품종 선발 ... 93
• 2. 대량증식기술 개발 ... 102
• 3. 대량생산 재배기술 종합실증 연구 ... 107
• 제4장 목표달성도 및 관련분야에의 기여도 ... 117
• 제1절 목표 달성도 ... 117
• 제2절 관련분야의 기여도 ... 120
• 제5장 연구개발결과의 활용계획 ... 122
• 제1절 연구개발결과의 활용 방안 ... 122
• 제2절 메드빌의 사업화 추진방안 ... 124
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 125
• 제1절 두릅나무를 활용한 제품에 대한 일본 정보 ... 125
• 제7장 참고문헌 ... 127
• 끝페이지 ... 132