초록 ▼

○ 연구결과
수확후 메밀새싹의 적정 전처리방법으로서 차아염소산염, 산성전해수, 이산화염소 처리로 1 log cycle 이상 미생물 저감효과를 얻을 수 있었으며 품질유지에도 효과적이었다. 메밀새싹의 품질유지에 적합한 온도조건은 10℃이었으며, 포장방법으로는 밀봉용기 포장구가 가장 효과적이었다. 재배공장에서 100 ppm 염소수 세척과 헹굼, 예냉, 포장 처리를 한 결과, 적용된 수확후 처리공정이 메밀새싹의 유통 중 신선도 유지에 긍정적인 영향을 미치면서 미생물 안전성 향상에 매우 효과적이었다. 상용 PCM(RT-2, SAP)을

○ 연구결과
수확후 메밀새싹의 적정 전처리방법으로서 차아염소산염, 산성전해수, 이산화염소 처리로 1 log cycle 이상 미생물 저감효과를 얻을 수 있었으며 품질유지에도 효과적이었다. 메밀새싹의 품질유지에 적합한 온도조건은 10℃이었으며, 포장방법으로는 밀봉용기 포장구가 가장 효과적이었다. 재배공장에서 100 ppm 염소수 세척과 헹굼, 예냉, 포장 처리를 한 결과, 적용된 수확후 처리공정이 메밀새싹의 유통 중 신선도 유지에 긍정적인 영향을 미치면서 미생물 안전성 향상에 매우 효과적이었다. 상용 PCM(RT-2, SAP)을 이용한 새로운 온도감응 기능성 포장재로서 유연성 보냉 포장재를 설계 제작하여 새싹 소포장에 적용한 결과, 일반 얼음팩 보냉재에 비해 내용물의 저온유지에 효과적이며, SAP 처리구의 내외부 온도가 RT-2에 비해 더 낮게 유지되었다. 능동형 MAP를 적용하였을 때 고 O2와 고 CO2의 혼합조건보다 60% 이하의 고 O2 조건이 메밀새싹의 품질유지에 효과적이었으나, 병원성 미생물(E. coli O157:H7, S. Typhimurium, S. aureus, L. monocytogenes)의 생균수 감소에는 효과적이지 못하였다. 생산현장에서의 실증시험으로부터 40% 고 O2 조건의 능동형 MAP 처리와 유연성 SAP 보냉재를 병용하여 포장했을 때 10-12시간 이내의 중단거리 유통과정에서 메밀새싹의 미생물 증식을 억제하고 초기품질을 최대한 유지할 수 있는 것으로 판단되었다.

Abstract ▼

Ⅳ. Results and Suggestion
The basic physiological and quality characteristics of buckwheat sprout showed that its respiration rate increased exponentially according to increment of environment temperatures (5-30℃) with maintaining normal aerobic respiration. The number of mesophilic and coliforms

Ⅳ. Results and Suggestion
The basic physiological and quality characteristics of buckwheat sprout showed that its respiration rate increased exponentially according to increment of environment temperatures (5-30℃) with maintaining normal aerobic respiration. The number of mesophilic and coliforms bacteria existed buckwheat sprout was the highest at the mature stage and it tended to decrease in the overmature stage of buckwheat sprout. In particular, loss of quality was observed at the overmature stage with blooming in overmature buckwheat sprout. To maintain best quality of buckwheat sprout, it was necessary to prevent the direct contact between buckwheat sprout and insulated material due to chilling injury occurred at -13℃ in 1 hour. In appropriate postharvest pre-treatment of buckwheat sprout experiment, hypochlorite solution (100 ppm), acidic electrolyzed water (pH 2.3), and chlorine dioxide (40 ppm) treatment brought to microbial decrement over 1 log cycle and maintained good quality during cold storage. To maintain best quality of buckwheat sprout, storing temperature should be 10℃ and sealed packaging in plastic tray was the most beneficial. When chlorine washing & cleaning (100 ppm), pre-cooling, packaging were performed separately in seed sprouts manufacturing step, maintaining freshness was not affected during distribution of buckwheat sprout and microbial safety was also effectively increased. A mathematical model was developed to predict the thermal experience of the seed sprout in expanded polystyrene box during transportation and delivery. The model was derived using basic heat transfer principles covering convection, conduction and radiation.
The temperature profiles of a simulant were recorded and the overall heat transfer coefficients were determined under various ambient temperatures (25, 30 and 35℃). The simulation model was confirmed by comparing predicted temperatures with measured values.
The new temperature-sensitive functional materials using commercial PCM (i.e., RT-2, SAP) with the flexibility was used to design and make cold retention package which was applied to buckwheat sprouts small package showed better result to maintain the low temperature of sample with the virtual summer air temperature condition compared to the general ice-cold pack. SAP treated sample's internal and external temperature remains low compared to RT-2 and as a result SAP's cold retention capacity was better than RT-2. In addition, varying the weight of a rectangular SAP cold retention materials, the greater the weight of cold retention materials in the package the internal temperature was maintained at a lower temperature for longer periods. In particular, even though the weight of SAP and commercial ice-pack was the same, application forms such as full surround with SAP was more effective to keep raw material temperature and quality. The result of using flexible SAP cold retention materials for buckwheat sprouts shipping containers to the actual distribution from the production, internal temperature of buckwheat sprout container tray was maintained lower than 1 ℃ for approximately 13 hours. In addition, there was no significant difference from existing conventional ice-pack in terms of the quality factors of seed sprout. The effects of phase change materials (PCM) on sprout temperature were analyzed. The phase change temperatures for MPA, SAP, and RT-2 ranged 1-2℃, 1-2℃ and 2-6℃, respectively. Among three types of PCMs, SAP was the most effective for retardation of temperature increase. Three-dimensional temperature profiles were obtained with various boundary and initial conditions using COMSOL multi-physics program. The simulation results showed good agreements with the experimental data.
To ensure microbial safety and stability of seed sprouts, various active MAP treatments were applied. Maintaining quality of buckwheat sprouts was better with lower 60 percent oxygen condition than the mixture of high oxygen and carbon dioxide. However, inoculated pathogenic bacteria(E. coli O157:H7, S. Typhimurium, S. aureus, L. monocytogenes) was not effective in reducing viability.
When active-MAP condition which effectively maintain the quality of buckwheat sprouts and flexible SAP cold retention materials treated in parallel, maintaining lower temperature of flexible SAP cold retention materials was less than 10 hours compared to regular ice packs. The number of aerobic mesophilic bacteria was tended to decrease slightly in most sealed packaging samples and sensory evaluation of decoloration and apparence was the best at 20% and 40% of the MAP high oxygen condition. The results in the actual production field showed that mid-long distance which takes less than 10-12 hours distribution could be possible to maintain initial quality and inhibit microbial growth when active MAP with 40% high oxygen conditions and flexible SAP cold retention materials treated in parallel. An analytical model was developed to predict a minimum distribution time for the sprout. The optimum combination of the insulation packaging materials and modified atmospheres was evaluated as well. The developed models can predict successfully the sprout temperature under various packaging conditions and can be used for packaging system design.

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 요 약 문 ... 3
• SUMMARY ... 7
• CONTENTS ... 12
• 목 차 ... 13
• 제 1 장 연구개발과제의 개요 ... 15
• 제 1 절 연구개발의 목적 ... 15
• 제 2 절 연구개발의 필요성 ... 15
• 제 3 절 연구개발의 내용과 범위 ... 18
• 제 2 장 국내외 기술개발 현황 ... 21
• 제 1 절 신선 농식품의 품질관리기술 ... 21
• 제 2 절 신선 농식품의 단열포장기술 ... 27
• 제 3 장 연구개발 수행 내용 및 결과 ... 28
• 제 1 절 연구개발 수행방법 ... 28
• 제 2 절 실험 재료 및 방법 ... 31
• 1. 새싹채소 시료 및 화학 약제 ... 31
• 2. 미생물 균주 및 선택배지 ... 31
• 3. 미생물 배양 및 접종 ... 31
• 4. 기능성 포장재 준비 ... 32
• 5. 새싹채소 시료 처리방법 ... 33
• 6. 새싹채소 특성 분석 ... 35
• 제 3 절 연구 내용 및 결과 ... 38
• 1. 새싹채소의 생리특성 및 품질특성 분석 ... 38
• 2. 새싹채소의 수확후 적정 전처리방법 탐색 ... 42
• 3. 환경조건 및 포장방법에 따른 새싹채소의 저장성 평가 ... 51
• 4. 기존 새싹채소 유통방식의 온도유지 효과 분석 ... 58
• 5. 새싹채소의 적정 수확후 처리 현장적용 실증시험 ... 65
• 6. 온도감응 기능성 포장재의 설계 및 제조 ... 70
• 7. 새싹채소에 대한 온도감응 기능성 포장의 적용성 평가 ... 73
• 8. 온도감응 기능성 포장의 유통 중 온도유지 효과 분석 ... 81
• 9. 온도감응 기능성 포장의 현장적용 실증시험 ... 98
• 10. 새싹채소에 대한 능동형 MAP 처리조건 설정 및 적용성 평가 ... 102
• 11. 새싹채소에 대한 온도감응/능동형 MAP 병용처리 효과 분석 ... 117
• 12. 유통 중 품질 영향인자의 변동 분석 및 예측 ... 125
• 13. 능동형 MAP 처리의 현장적용 실증시험 ... 140
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 147
• 제 5 장 연구개발 성과 및 성과활용 계획 ... 148
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 149
• 제 7 장 참고문헌 ... 173
• 부록: 호주 새싹채소의 주요 생산 및 가공 규격(안) ... 186
• 끝페이지 ... 242