초록 ▼

○ 연구결과
새로운 PEDV주를 분리하고자 하는 노력의 일환으로 돼지조직으로부터 초대배양세포의 구축을 실시하였고,telomerase의 과발현을 통한 세포의 불멸화 유도를 통하여 여러 가지 초대배양 세포의 구축을 실시하였다.또한 보다 효율적인 PEDV 백신을 위하여,새로운 야외주의 분리 성과를 이루었고,다양한 형태의 효율적인 백신 개발 가능성 모색을 위한 연구 결과로써, PEDV의 중화항체 유도부위 (neutralizing epitope)부위를 확정 하였으며,이를 이용한 invivo실험을 실시하였다.또한 PEDV에 대한 보다 정

○ 연구결과
새로운 PEDV주를 분리하고자 하는 노력의 일환으로 돼지조직으로부터 초대배양세포의 구축을 실시하였고,telomerase의 과발현을 통한 세포의 불멸화 유도를 통하여 여러 가지 초대배양 세포의 구축을 실시하였다.또한 보다 효율적인 PEDV 백신을 위하여,새로운 야외주의 분리 성과를 이루었고,다양한 형태의 효율적인 백신 개발 가능성 모색을 위한 연구 결과로써, PEDV의 중화항체 유도부위 (neutralizing epitope)부위를 확정 하였으며,이를 이용한 invivo실험을 실시하였다.또한 PEDV에 대한 보다 정확한 진단 시스템 구축을 위한 노력의 일환으로 보다 효율적으로 이 질병을 진단할 수 있는 진단 kit개발에 노력하였다.

Abstract ▼

Porcine epidemic diarrhea (Porcine Epidemic Diarrhea, PED) is one of the most important viral disease in swine industry in asian countries, including South Korea. This disease occurred specifically piglets prior to weaning with severe diarrhea and dehydration, especially in the winter. However, this

Porcine epidemic diarrhea (Porcine Epidemic Diarrhea, PED) is one of the most important viral disease in swine industry in asian countries, including South Korea. This disease occurred specifically piglets prior to weaning with severe diarrhea and dehydration, especially in the winter. However, this disease is currently endemic in the winter than other seasons, and disease patterns are also much more likely to be encountered. Discrepancy of vaccine effectiveness might be came from genetic difference in PEDV and antigenicity difference are more likely to cause problems. Because of these reasons, development of new vaccines using wild type PEDV is absolutely required, we have been tried to do our best to solve these issues.
With groups of experts in this field, we established couple of immortalized cell lines using primary swine tissues. Those cell lines were stabilized and tested for susceptibility for PEDV. PEDV was successfully propagated on these new cell lines and we tried to isolate wild type PEDV using these cell lines.
We also tried to prove specific neutralizing epitope on PEDV which could be useful for development of effective vaccines. We found specific neutralizing epitope is located on tail domain on spike protein. This is very new and interesting findings because it is the first finding on this region as the neutraling epitope. We also proved that this region is quite promising neutralizing in vivo experiments. This results might be useful for new generation of PEDV vaccine for the future as recombinant vaccine.
We also tried to prove receptor protein of PEDV using many other experiments. This is one of the most important issues on PEDV and we used different, different approaches for this study.
We found CD13 might be very promising receptor protein for PEDV infection and this results are also one of our great finding from this study. In pathogenesis studies, we found PEDV also successfully infect alveolar macrophages and this is quite surprising results as well. We found PEDV infection seems like on going on alveolar macrophages in infected piglets. Although we don't know yet clearly what this means, but at least we know, this might be very new finding and should have special meaning on understanding of pathogenesis.
We also developed couple of monoclonal antibodies on PEDV, and successfully developed detection kit for PEDV using those. We already commercialized this kit for the market.
All our results and performance was quite useful and efficient expecially on understanding of PEDV. We successfully isolated new PEDV strains using primary swine cell lines and tried to develop new vaccines. We also developed new efficient detection kit for PEDV. Those development might useful for efficient detection and protection for PEDV in piglets and this might be very useful for swine industry.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY(영문요약문) ... 6
• CONTENTS(영문목차) ... 7
• 목차 ... 8
• 제1장 연구개발과제의 개요 ... 8
• 제1절. 연구개발의 목적 ... 8
• 제2절.연구개발의 필요성 ... 9
• 제3절.연구개발의 범위 ... 13
• 제2장 국내외 기술개발 현황 ... 15
• 제3장 연구개발수행 내용 및 결과 ... 20
• 제1절 중화항체 유도부위를 이용한 효율적인 PEDV 백신 개발 ... 20
• 제2절 초대배양세포주를 이용한 새로운 PEDV 야외주 분리 및 백신주 개발 ... 40
• 제3절 PEDV 백신의 효율성 검증 및 효율성 검사 kit개발 ... 68
• 제4장 목표달성도 및 관련분야에의 기여도 ... 89
• 제5장 연구개발 성과 및 성과활용 계획 ... 90
• 1. 기대성과-기술적 측면 ... 90
• 2. 기대성과-경제·산업적 측면 ... 91
• 3. 활용방안 ... 91
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 91
• 제7장 참고문헌 ... 91
• 끝페이지 ... 98