초록 ▼

연구결과
필요성 및 목표
○ 모발중 대마성분 감정에 있어 효율적인 추출법 및 고감도 LC/MS/MS법을 이용하여 분석법을 확립
○ 최근 남용 및 약물강간사건 등에 사용되는 GHB의 모발에서의 분석법 확립으로 법과학적 증거확보에 기여
방 법 및 결 과
○ 대마성분의 추출법으로는 가수분해법, 용매을 이용한 액상추출법을 이용하였으며, LC/MS/MS법으로는 MS3 및 MRM을 동시에 적용하였고, 실제 모발시료에 적용.
○ GHB의 추출은 여러 가지 방법중 가수분해법 및 액상추출법을 이용하였으며, 유도체화를

연구결과
필요성 및 목표
○ 모발중 대마성분 감정에 있어 효율적인 추출법 및 고감도 LC/MS/MS법을 이용하여 분석법을 확립
○ 최근 남용 및 약물강간사건 등에 사용되는 GHB의 모발에서의 분석법 확립으로 법과학적 증거확보에 기여
방 법 및 결 과
○ 대마성분의 추출법으로는 가수분해법, 용매을 이용한 액상추출법을 이용하였으며, LC/MS/MS법으로는 MS3 및 MRM을 동시에 적용하였고, 실제 모발시료에 적용.
○ GHB의 추출은 여러 가지 방법중 가수분해법 및 액상추출법을 이용하였으며, 유도체화를 한 후 GC/MS법 이용
기대성과
○ 모발중 대마성분 검출시 새로운 분석방법으로 기존의 복잡하고 힘든 방법을 대체할 수 있음.
○ 모발중 GHB의 분석은 소변에서만 가능하였는데, 모발에서의 분석법 개발로 남용여부 및 약물관련성폭력 피해자에서의 투약여부 등을 판단할 수 있는 근거 확립

Abstract ▼

I. Cannabis
Identification of 11-nor-9-carboxy-tetrahydrocannabinol (THCCOOH) in hair can be an important proof of cannabis use because it can exclude the possibility of passive cannabis smoke exposure. In this study, we described a quantitative method of THCCOOH in hair using simple liquid-liqui

I. Cannabis
Identification of 11-nor-9-carboxy-tetrahydrocannabinol (THCCOOH) in hair can be an important proof of cannabis use because it can exclude the possibility of passive cannabis smoke exposure. In this study, we described a quantitative method of THCCOOH in hair using simple liquid-liquid extraction (LLE), selective column switching liquid chromatography with electrospray ionization (ESI)-MS³. For the column switching system three columns (precolumn, trap column and analytical column) were used. The internal standard was THCCOOH-d3. We used ESI-negative-MS³ transition of ions at m/z 343 to 299 to 245 (343/299/245) and m/z 346 to 302 to 248 (346/302/248) for quantification of THCCOOH and THCCOOH-d₃, respectively. The validation results of selectivity, matrix effect, recovery, linearity, precision and accuracy, and processed sample stability were satisfactory. The limit of detection (LOD) was 0.05 pg/mg and the limit of quantification (LOQ) was 0.10 pg/mg. The range of concentration of THCCOOH from 98 authentic human hair was 0.13 ~ 15.75 pg/mg.
II. GHB
We have developed a GC/MS assay for GHB in human hair. 20 mgs of hair samples were hydrolyzed by 1 N-NaOH before liquid-liquid extraction with ethyl acetate under acidic conditions. GHB-d6 was used as internal standard. TMS-derivatives were formed before injection. Analysis of basal 50 drug-free hair samples gave the following results: 1.39 ± 0.70 ng/mg(range : 0.60 ∼ 2.85 ng/mg). There was no relation with melanin pigmentation due to no difference between black and white hair in rat model. In that rat model, after 4 times consecutive administration of GHB made difference from blank hair. There were no GHB positive results in 20 cases who tried to buy GHB in internet markets.