초록 ▼

연구개발 결과
- National Center for Biotechnology Information (NCBI)에 축적된 남조류 속(genus)별 유전자의 염기서열 정보를 기본으로, 조류경보제 대상 남조류 5종(Microcystis, Anabaena, Oscillatoria, Phormidium, Aphanizomenon)에 대한 특이적 염기서열을 결정하였다.
- mcyE, cpcBA, ITS, rbcS, rbcX 또는 nifD 등 5개 유전자 대상으로, 각 유전자별 3개 부분에 대한 유전자 탐침(probe)을 이용하여

연구개발 결과
- National Center for Biotechnology Information (NCBI)에 축적된 남조류 속(genus)별 유전자의 염기서열 정보를 기본으로, 조류경보제 대상 남조류 5종(Microcystis, Anabaena, Oscillatoria, Phormidium, Aphanizomenon)에 대한 특이적 염기서열을 결정하였다.
- mcyE, cpcBA, ITS, rbcS, rbcX 또는 nifD 등 5개 유전자 대상으로, 각 유전자별 3개 부분에 대한 유전자 탐침(probe)을 이용하여 DNA chip을 제작함으로써, 통계적으로도 유의한 결과를 얻도록 하였다. 남조류 간, 박테리아 간 간섭이 유의하게 낮고 특이성이 있음을 확인하였으며, 종류별 일정 구간에서 정량 분석도 가능함을 확인하였다.
- 현행 조류경보제는 남조류의 동정과 계수 과정에 특별한 훈련과 많은 시간이 소요됨에도 불구하고, 객관성이 떨어지는 문제점이 제기되어 왔다. DNA chip은 이러한 문제점을 해결하고, 객관성과 신뢰성을 향상시킬 수 있다. 동시에 유해성 미생물과 다른 미생물 군집의 변화까지도 분석할 수 있어 수생태 연구에 획기적인 도구로 사용될 수 있다.
- 연구기간 동안 SCI 논문 26편 및 국내논문 6편 게재, 국내특허 12건과 국제특허 1건 출원 및 4건의 국내특허가 등록되었다. 총 39회 국내외 학회에서 연구내용을 발표하였으며, 총서 1권을 발간하였다. 언론에 녹조 관련 칼럼이 3회 보도되었으며, 관련 후속 프로젝트 1건을 유치하였다. 본 개발기술로 2012년, 2014년 한국환경산업기술원에서 환경기술 최우수상, 우수상을 각각 수상하였으며, 2014년 산업통상자원부 신기술인증(NET)을 획득하였다.

Abstract ▼

Ⅳ. Results
◦ Total
Based on the nucleotide sequence information accumulated in the National Center for Biotechnology Information in (NCBI), genus-specific sequences were developed (for Microcystis, Anabaena, Oscillatoria, Phormidium, Aphanizomenon). Three portions of sequences were chosen from

Ⅳ. Results
◦ Total
Based on the nucleotide sequence information accumulated in the National Center for Biotechnology Information in (NCBI), genus-specific sequences were developed (for Microcystis, Anabaena, Oscillatoria, Phormidium, Aphanizomenon). Three portions of sequences were chosen from each five target genes of mcyE, cpcBA, ITS, rbcS, and nifD or rbcX, to produce a DNA chip with sufficient statistical significance. Specificities between different genera were confirmed and quantitative correlations was also made between cyanobacteria and chip signals. The current alert system for algal bloom did not produce reliable and objective results, in spite of the time-consuming and laborious processes, requiring special training. DNA chip can resolve these issues. It may be also possible to analyze any changes in the microbial community and other harmful microorganisms, by using this innovative tool for the study of several ecosystems.
cDNA chip using Z. platypus liver was evaluated the exposure of endocrine disrupting chemicals in Miho stream, Korea. Bisphenol A and nonylphenol were detected 1.73 and 0.53 ug/L, respectively. However, those chemicals did not affect gene expressions in Z. platypus. The manuals for risk assessment in aquatic ecosystems were 1) the integrative tools for risk assessment in aquatic ecosystems 2) SOP for the test of cDNA chip in Z. platypus. Additionally, genes of Z. platypus liver were registered in Nucleotide BLAST (NCBI).

◦ Unit 1
The methods both Flat sheet Silicon Membrane Method and Purge & Trap are applied to Online VOC Monitoring System in water, together or apart. This system was tested both in laboratory and site before it was lunched in the market successfully. The Glow Discharge method is applied to trial product for Heavy Metal Online Monitoring System.
The performance of the microcystin detection instrument developed in this project was exellent. The detection limit was 0.35 ng/mL and the analysis time was 15 min.

◦ Unit 2
The typical monitoring of SOB biosensor is based on the SOB responses to physical, chemical or biological stimulation from the toxic chemicals which is usually accompanied by changes in pH and electrical conductivity (EC). SOB oxidize elemental sulfur to sulfuric acid under aerobic condition. The production of hydrogen ion lowers the medium pH and sulphate increases the EC of the medium. In the presence of toxic substances, production of sulphate will be inhibited and thus, EC will decrease or remain stable. Based on this methodology, we succeed to develop an online toxicity monitoring system. Our online toxicity system can monitor presence of heavy metals and other toxic chemicals in water from few minutes to hour.