초록

세계적으로 문제를 일으키는 다제내성균의 신속한 진단을 위해 새로운 진단법을 개발하고자 연구에 임하였으며, 1차에서 3차년도에 걸쳐 다제내성균 (MRSA,VRE, CRPA, CRAB, CRE)의 검체를 확보하여 배양 및 DNA 정제를 통해 신속 진단할 수 있는 기술인 등온증폭법 (LAMP)을 이용하여 신속 진단을 수행하였다. 또한, 실제 임상적인 상태에서 이용 가능하게끔 LAMP 반응 플랫폼을 개발하고자 함.

(출처 : 요약서)

Abstract ▼

Ⅰ. Research goal and objectives
To develop a rapid diagnostic method for multidrug-resistant bacteria causing trouble worldwidely, the most effective DNA extraction method for various kinds of multidrug-resistant bacteria and clinical specimens is estimated and established. Directly extracted DNA

Ⅰ. Research goal and objectives
To develop a rapid diagnostic method for multidrug-resistant bacteria causing trouble worldwidely, the most effective DNA extraction method for various kinds of multidrug-resistant bacteria and clinical specimens is estimated and established. Directly extracted DNA from clinical speicimens is tested by LAMP (Loop-mediated isothermal amplification) as rapid diagnostic method. Early detection of drug-resistant gene can help clinicians choose a better antibiotic treatment regimen, which will eventually reduce social costs caused by multidrug-resistant infection.

Ⅱ. Reseach progress
1. Culture of multidrug-resistant bacteria (MRSA, VRE, CRPA, CRAB, CRE), the collection of clinical specimen and the DNA purification
? culture collection of clinical specimens
? the selection of clinical specimens for testing a rapid diagnostic method
? The establishment of DNA extraction method of clinical specimens for LAMP
? DNA purification of multidrug-resistant bacteria
2. The optimization of LAMP reaction for rapid diagnosis
? The analysis of full sequence of drug-resistant gene each target strain (mecA, vanA, blaOXA-23, blaVIM-2, blaIMP-6, blaNDM-1, blaKPC-2)
? The establishement of LAMP primer set targeting the most sensitive resistant gene for each target strain.
? The optimization of LAMP reaction based on using magnetic bead
? The optimization of multiplex LAMP
3. The development of LAMP reaction platform is available to clinical field
? The optimization of LAMP master reagent mixture targeting each drug-resistant gene
? The development of final platform for rapid method through Multiplex.
4. The clinical estimation of LAMP for drug-resistant bacteria

Ⅲ. Performance expectation followed by reseach development
? collect and isolate various kinds of multidrug-resistant bacteria and clinical isolates causing the infection of super bacteria,
? monitor the samples to detect durg-resistance tendency
? select more effective DNA extraction method for molecular diagnostic technique
? develop a platform technique for detecting the presence of specific drug-resistant gene from patients within a matter of hours by optimizing LAMP of drug-resistant bacteria for rapid diagnosis
? The verifiation of developed LAMP by preclinical trials
? Effective measures and the preparation of rapid method for identifying the disseminating multidrug-resistant bacteria will facilitate early detection of the emergence of multidrug-resistant bacteria.

(출처 : SUMMARY)

목차 Contents

• 표지 ... 1제출문 ... 2보고서 요약서 ... 3요약문 ... 4SUMMARY ... 56.1 총괄연구개발과제의 연구성과 실적 및 향후 계획 ... 66.2 연구성과 유형별 세부 내역 ... 77. 참여연구원 현황표 ... 15II. 총괄연구세부1과제 연구결과 ... 16 1. 연구개발과제의 배경 및 필요성 ... 17 2. 국내외 기술개발 현황 ... 18 3. 연구개발과제의 추진체계 ... 20 4. 연구개발수행 내용 및 결과 ... 21 5. 목표달성도 및 관련분야 기여도 ... 73 6. 향후 연구성과 추진 계획 ... 75 7. 연구개발결과의 파급효과 ... 75 8. 연구개발결과의 활용계획 ... 76 9. 연구개발과정에서 수집한 해외과학기술정보 ... 76 10. 참고문헌 ... 76IV. 첨부서류 ... 81끝페이지 ... 119