초록 ▼

○ Sepbox 및 HTS 시스템을 개발
○ 소청룡탕 처방 1종, 구성 약재 등 개별생약 대상 초고속 성분 분석 완료 및 생리 활성 탐색 완료
○ 소청룡탕 표준화 완성
○ 표준한약소재 15종(감초, 건강, 계지, 길경, 마황, 맥문동, 반하, 백출, 사삼, 세신, 오가피, 오미자, 작약, 포공영, 황기) 확보 및 추출 물 172종 제조(자생식물 81종, 일반약재 91종)
○ 기원별, 산지별, 포제방법별 표준한약소재 원물 103종 확보
○ 자생식물 288종 기원 효능주치와 동의보감 한약재 300종 기원 효능

○ Sepbox 및 HTS 시스템을 개발
○ 소청룡탕 처방 1종, 구성 약재 등 개별생약 대상 초고속 성분 분석 완료 및 생리 활성 탐색 완료
○ 소청룡탕 표준화 완성
○ 표준한약소재 15종(감초, 건강, 계지, 길경, 마황, 맥문동, 반하, 백출, 사삼, 세신, 오가피, 오미자, 작약, 포공영, 황기) 확보 및 추출 물 172종 제조(자생식물 81종, 일반약재 91종)
○ 기원별, 산지별, 포제방법별 표준한약소재 원물 103종 확보
○ 자생식물 288종 기원 효능주치와 동의보감 한약재 300종 기원 효능주치 정보 라이브러리 완료
○ 연계소재-MC 기기분석법 확립 (복합물 3종, 개별약재 10종 이상)
○ 소재 판별분석법 개발
○ MC 간접정량법과 MC 지문분석법 확립
○ 연계소재 적용을 통한 검증 및 가공공정 개발을 통 한 새로운 후보 소재 도출
○ 대사체 프로파일링 기반 전통천연물 소재 판별 시스템 구축 및 MC 규격 설정
○ 대사체간 상호작용 분석시스템 구축
○ 전통천연물처방 복방 및 단방의 대사체 (활성성분) 프로파일링 비교분석 및 상호작용 규명
○ 대사체 (활성성분) 분리 및 표준품 확보

(출처 : 보고서 요약서 5p)

Abstract ▼

IV. Result
< First group>
○ Integrating high throughput fractionization system (THF), high throughput screening (HTS), high concents screening (HCS), and high affinity high resolution mass spectrometry (HAMS), naming iHTac successfully completed.
○ Completion of standardization for Socheong

IV. Result
< First group>
○ Integrating high throughput fractionization system (THF), high throughput screening (HTS), high concents screening (HCS), and high affinity high resolution mass spectrometry (HAMS), naming iHTac successfully completed.
○ Completion of standardization for Socheongnyongtang.
○ High throughput component analysis using iHTac system
- Fractions from Socheongyongtang and HT057 are secured.
- Separation and structure identification of 26 pure compounds from Socheongnyongtang.
- Completion of high throughput component analysis of Socheongyongtang and related natural products.
○ MC/MT bioactivity investigation
- Research on anti - inflammatory effect of Socheongyongtang
- Research on individual natural products for enhancement of Socheongyongtang Research on anti-inflammatory effect of HT057 against inflammatory bowel disease
- Research of natural products for anti-cancer adjuvant therapeutic effect
- Research of novel natural product ’LB’ on anti-inflammatory effect
- Research on anti-fatigue effect of HT071

< Second group>
○ Selecting and supplying effective herbs to respiratory diseases, collitis, fatigue, cold, and COPD
○ Collecting 15 standard natural product materials: Glycyrrhiza root, Zingiber Rhizome, Cinnamomi Ramulus, Platycodon root, Ephedra Herb, Liriope Tuber, Pinellia Tuber Pinellia Tuber, Atractylodes Rhizome White, Adenophora Root, Asiasarum Root Asiasarum Root and Rhizome, Acanthopanax Root Acanthopanax Root Bark, Schisandra Fruit, Peony Root, Tarax Herb, Astragalus Root
○ Supplying the samples (herb material, extract)
○ Updating TKM information of 588 herbs in the Info library
○ Collecting 495 native plants, and making voucher specimen
○ Collecting 204 current herbs, and indetifying
○ Making 172 extracts
○ Establishing the regulations and parcel procedures of the material library

< Third group>
○ Development of discrimination techonology based on the metabolomics for identification of traditional herbal medicine origin, cultivar, mixing ratio. The current identification methods, sensory inspection, protein pattern analysis, and genetic test, were complemented, and the discrimination technology for mixing ratio is the unique one of our research. By using these technologies, the accuracy and reliability of our research increased.
○ Establishment of standard herbal medicine the experimental variables were optimized by using RSM for the establishment of analytical method, which can supply MC selective analysis in the practical system. During the first stage, MC profiling DB of traditional herbal medicine or prescriptions was established. Drawing MC distribution map and selection of reference chemical profile were performed.
○ Development of statistical algorithm for accurate MC chemical equivalence the current algorithm was able to show the chemical equivalence of material partially, whereas the new algorithm is possible to show the more accurate chemical equivalence including the evaluation of proportion of MC and their contents.
○ Application to the specific material (co-research) HT057, HT071, So-Chung-Ryong-Tang
○ Development of other technologies the relative Quantification method, possible to perform MC Quantification without the standards, was created and the interpretation algorithm was established.
○ Industrialization of traditional prescription the protocol for removing the toxic substances in So-Chung-Ryong-Tang was optimized to use it safely.

< Forth group>
○ We have identified and Quantified the active components in the So-cheong-ryong-tang (SCRT) using ultra performance liquid chromatography Quadrupole time of flight mass spectrometry (UPLC-QTOF-MS) system. Using our optimized method that can simultaneously analyze eight active compounds, we have ranked SCRT with different origin, production center, decoction process, and etc based on the Quantity of the active compounds. During this process, we have identified that higher ranked SCRT has greater amount of (2E,4E,8Z,10E)-N-isobutyldodeca-2,4,8,10-tetraenamide, an active compound that is found in Asari Radix et Rhizoma which is one of the components in SCRT. Also, we have set-up a guideline for multi-components in HT071 and HT057 through metabolite profiling study. Furthermore, we have developed a method to analyze the interactions of the multiple components in SCRT in a CYP450 system using Gas Chromatography-Mass Spectrometry (GC-MS).

(출처 : SUMMARY 15~16p)

목차 Contents

• 표지 ... 1
• 제출문 ... 3
• 보고서 요약서 ... 5
• 요약문 ... 7
• SUMMARY ... 13
• CONTENTS ... 19
• 목차 ... 21
• 제 1 장 연구개발과제의 개요 ... 23
• 제 1 절 연구개발의 목적 ... 23
• 제 2 절 연구개발의 필요성 ... 24
• 제 2 장 국내외 기술개발 현황 ... 33
• 제 3 장 연구개발수행 내용 및 결과 ... 49
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 385
• 제 1 절 연구개발목표의 달성도 ... 385
• 제 2 절 관련분야의 기술발전에의 기여도 ... 391
• 제 3 절 기타 ... 396
• 제 5 장 연구개발결과의 활용계획 ... 401
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 및 참고문헌 ... 409
• 끝페이지 ... 418