Abstract ▼

Stress is a major determinant of quality of life. With the rapid increase in the proportion of the aging population, the market for medicines used for treatment of mental illnesses, including anti-depressants and tranquilizers, has shown a rapidly growing trend. The existing chemical compound-based

Stress is a major determinant of quality of life. With the rapid increase in the proportion of the aging population, the market for medicines used for treatment of mental illnesses, including anti-depressants and tranquilizers, has shown a rapidly growing trend. The existing chemical compound-based drugs for treating depression have various adverse effects, such as drowsiness, nausea, and vomiting. Hence, the aim of the present study was to identify candidate medicinal entities that are effective against anxiety and depression from the herbal medicines with proven efficacy and safety.

For the selection of the herbal candidates, 29 varieties of extracts were classified according to their pharmacological properties and efficacy, by a combined prescription survey. Of these, 13 effective herbs were selected on the basis of results of corticosterone treatment and the transcription factor expression profile in cell model system. The herbal medicine(HSF, HSR) administered group showed greater protective effects against the corticosterone-induced cytotoxicity in neurons than the control group, and exhibited significant anti-oxidative and anti-inflammatory effects, as well as stress hormonal and neurotransmitter activities.

Efficacy of the herbal medicines was evaluated by animal behavioral experiments, using immobilization stress to induce depression. No significant changes in body weight were observed between the depression-induced animals in the herbal-medicine-administered and the control group. In the behavioral tests, the herbal-medicine-administered group showed antidepressant-like activity in the sucrose consumption test(SPT), the forced swimming test(FST) and the tail suspension test(TST), as compared with the control group. The depression-induced animals also showed significantly decreased blood levels of the stress hormone, cortisol, and significantly increased blood levels of serotonin, as compared with the control group.

In conclusion, the herbal candidates, selected first on the basis of in vitro cytoprotective effects and their transcription factor expression profile and further on the basis of in vivo effects on anxiety and depression in stress-induced animal models, were identified as entities for development into medicines for improving and treating stress-induced depression and anxiety. These findings may be used as basic data for the future development of such material for use as natural medicines.

( 출처 : SUMMARY 10p )

목차 Contents

• 표지 ... 1
• 제 출 문 ... 3
• 보고서 초록 ... 5
• 요 약 문 ... 7
• SUMMARY ... 10
• CONTENTS ... 11
• 목차 ... 12
• 제 1 장 연구개발의 목적 및 필요성 ... 13
• 제 1 절 연구개발의 목적 ... 13
• 제 2 절 연구개발의 필요성 ... 13
• 제 2 장 연구개발의 내용 및 범위 ... 14
• 제 1 절 연구개발의 내용 ... 14
• 제 3 장 연구개발방법 ... 17
• 제 1 절 세포모델에서의 세포활성 및 전사인자발현시스템을 이용한 효능평가 ... 17
• 제 2 절 스트레스로 인한 동물모델에서의 한약효능평가 ... 20
• 제 4 장 연구개발결과 ... 23
• 제 1 절 세포모델에서의 세포활성 및 전사인자발현시스템을 이용한 효능평가 ... 23
• 제 2 절 스트레스 유발 동물모델에서의 한약효능 평가 ... 40
• 제 3절 한의이론 기반 약리결과와의 연관성 조사 ... 49
• 제 5 장 연구개발결과의 활용계획 ... 52
• 참 고 문 헌 ... 53
• 끝페이지 ... 55