초록 ▼

○ 논문게재(SCI): 8건(익힌 숙잠 제조 및 성분분석 2편, 기능성 구명 6편)
○ 논문게재(비SCI): 11건(익힌 숙잠 제조법 3편, 성분구명 1편, 기능성 구명 7편)
○ 특허출원: 7건(파킨슨병 예방, 간질환 예방, 피부미백효과 등)
○ 특허등록: 3건(파킨슨병 예방, 알코올성 간염, 위염 예방효과 등)
○ 학술발표: 17건(익힌 숙잠 기술개발, 성분구명, 기능성 구명 등)
○ 기술이전: 4건(파킨슨병 예방, 알코올성 질환예방 등)
○ 영농활용기관제출: 2건(익힌 숙잠 생산기술 등)

○ 논문게재(SCI): 8건(익힌 숙잠 제조 및 성분분석 2편, 기능성 구명 6편)
○ 논문게재(비SCI): 11건(익힌 숙잠 제조법 3편, 성분구명 1편, 기능성 구명 7편)
○ 특허출원: 7건(파킨슨병 예방, 간질환 예방, 피부미백효과 등)
○ 특허등록: 3건(파킨슨병 예방, 알코올성 간염, 위염 예방효과 등)
○ 학술발표: 17건(익힌 숙잠 기술개발, 성분구명, 기능성 구명 등)
○ 기술이전: 4건(파킨슨병 예방, 알코올성 질환예방 등)
○ 영농활용기관제출: 2건(익힌 숙잠 생산기술 등)
○ 기준/지침/규정작성: 1건(익힌 숙잠 통상 실시 건 허여기준)
○ 농가기술지도/컨설팅/현장기술지원: 4건(익힌 숙잠 기술이전 농가, 업체 등 기술지원)
○ 홍보성과: 362점 (알콜올성 질환 예방, 피부미백효과 등)

(출처 : 요약서 3p)

Abstract ▼

Purpose&Contents
□ Purpose
○ The elucidation the health improvement effects of steamed and freeze-dried mature silkworm (SMSP) in healthspan, cognition, movement, skin care, alopoecia and liver functions and underlying mechanisms.
○ Development detailed SMSP production techniques and spread

Purpose&Contents
□ Purpose
○ The elucidation the health improvement effects of steamed and freeze-dried mature silkworm (SMSP) in healthspan, cognition, movement, skin care, alopoecia and liver functions and underlying mechanisms.
○ Development detailed SMSP production techniques and spread them to the farmers and studies on characteristics and components of SMSPs generated by several silkworm strains.
○ Creation of new farm income source by creating popular demand using high-value-added SMSPs

□ Contents
○ The improvement effects of SMSP on cognitive and movement functions
- The effects of White Jade strain (also known as Baekokjam) SMSP in extension of healthspan
* The average life span and helathspan of SMSP food raised flies were 10% and 75% increased compared with normal food raised flies, respectively.
- The inhibitory effects of SMSP in the onset of Parkinson’s disease(PD)
* SMSP prevented the onset of rotenone induced PD in mouse and Drosophila models.
- Comparing the health improvement effects of 4 different SMSPs (particel sizes < 0.1mm) pulverized by Hammer mill.
* White jade strain and red silk strains had most significant effects in health improvements
* 4 different SMSPs enhanced resistances to PD
* Golden silk SMSP showed most significant effects on the prevention of the onset of PD
* Golden silk SMSP improved the synaptic architectures of DJ-1 PD Drosophila model.
- Comparison health improvement effects of 4 different SMSPs puliverized by the roller mill (particle size < 0.01mm)
* 4 SMSPs had effects of extension of healthspans: Golden silk > White jade > Red Silk > Yeonokjam
* 4 SMSPs prevented the onset of PD: Golden silk > red silk > White jade > Yeonokjam
- The cognitive function improvement effects of SMSP
* SMSP prevent the loss of short term memory induced by scopolamine in mice
* SMSP improved the synaptic architectures of Alzheimer model Drosophila to normal Ddrosophila
* 4 SMSPs had effects on synaptic architectures in AD model Drosophila. Golden silk and White jade were two more significant effects.
- The mechanisms underlying the prevention of onset of PD by SMSP
* The activities of respiratory complex 1 ~ 4 in mitochodria in SMSP feded Drosophila were significantly increased.
* The SMSP feded Drosophila had superior olfactory abilities compared with normal food fed Drosophila.

○ Development of skin care health supplements using SMSP
- In vivo skin whitening and hair growth promoting activity of White Jade strain SMSP
* SMSP showed skin whitening activity (increase in L value significantly vs. control) when administered orally and hair growth promoting activity when applied topically (increase in hair weight significantly vs. control), respectively.
- It was not clear that White Jade strain SMSP has in vivo anti-wrinkle or moisturizing activity.
- Pistachio strain SMSP showed the most potent skin whitening activity among four SMSP strains when administered orally.
* Pistachio strain SMSP reduced skin pigmentation about 41% when administered orally.
- Pistachio strain SMSP showed the most potent hair growth promoting activity among four SMSP strains when applied topically.
* Pistachio strain SMSP increased hair weight about 1.8 times compared with control when applied topically.
- Determination of the appropriate dose of the Pistachio strain SMSP for the skin whitening and hair growth promoting activities
* Skin whitening activity: 0.5-1 g/kg, hair growth promoting activity: 0.7-1 g/kg
- Determination of the safety of the Pistachio strain SMSP for long-term use and its acute single dose toxicity
* No significant differences in serum biochemical parameters, organ weights, and necropsy findings between control and SMSP-treated groups
- Obtaining tablets for oral administration (skin whitening) and a formulation for topical application (hair loss prevention) for the Pistachio strain SMSP

○ The elucidation of liver function improvement effects of SMSP
- Investigation of SMSP on the prevention of hangover symptoms
* Identification of the effect of Baekokjam mature silkworm powder on blood alcohol and acetaldehyde concentration inhibition and the prevention of liver damage by alcohol
* Comparison of the efficacy of hangover prevention for three kinds of Baekokjam, Golden silk and Yeonnokjam maure silkworm powder showed the best efficacy for prevention of hangover and alcohol-induced liver damage in Baekokjam varieties
* As a result of the hourly administration, it was confirmed that the inhibitory effect of Baekokjam mature silkworm powder on the blood alcohol and acetaldehyde concentration was the most excellent in the administration 30 minutes before the alcohol consumption
- Investigation of mature silkworm powder on the prevention of alcoholic fatty liver diseases
* Identification of the effect of Baekokjam mature silkworm powder on the prevention of liver cirrhosis, fatty liver and liver injury induced by ethanol
- Investigation of mature silkworm powder on the prevention of alcoholic gastric damage (conducted research that is not in the plan)
* Comparison of the efficacy of gastritis prevention for three kinds of Baekokjam, Golden silk and Yeonnokjam maure silkworm powder showed the best efficacy for prevention of alcohol-induced gastric damage in Baekokjam varieties
* Identification of the effect of Baekokjam mature silkworm powder on the prevention of gastric damage induced by ethanol through inhibition of inflammation response and restoration of gastric mucosal damage
- Investigation of mature silkworm powder on the improvement of liver function in non-alcoholic liver disease
* Comparison of the efficacy of hepatitis prevention for three kinds of Baekokjam, Golden silk and Yeonnokjam maure silkworm powder showed the best efficacy for prevention of diethylnitrosamine-induced liver damage in Baekokjam varieties through inhibition of inflammatory T cell activation
- Investigation of mature silkworm powder on the prevention of liver cirrhosis and liver cancer
* Identification of the effect of Baekokjam mature silkworm powder on the prevention of liver cirrhosis and hepatocellular carcinoma induced by diethylnitrosamine

○ The development of SMSP production methods and elucidating their characteristics
- Investigate to prevent of Steamed Mature Silkworm spewing thread condition
* Invention of minimize spewing method by hydro-thermal treatment and boiled water treatment in Steamed Mature Silkworm
- Development of effective hydro-thermally treated method for mass production of farmhouse
* Through steamer application technology development, reduces spewing time by 1/2 and increases the volume of material by 10 times the -> It is possible twenty time a day
- Cultivating the silkworm varieties for Steamed Mature Silkworm
* Development of a Roller-Mill grained method for easy grinding and have no loss consistent component
- Studies of Steamed Mature Silkworm functionally composition
* Confirm that it is containing variety of functional component and large amounts of amino acids and Ω3 fatty acids (35 %), including protein (70 %), glycine (12 %) alanine (10 %), serine (5 %) aspartic acid (5 %) and tyrosine (5 %) and etc
- Difference by grinding method in Steamed Mature Silkworm component
-Outstanding component content of roller-mill grinding specimen rather than hammer-mill grinding specimen
* The defect of hammer-mill grinding specimen is combined with themselves, The settlement difficulty of fine powder and the distortion of ingredients.
- Investigate of functionality components such as DNJ content and the pepsin digestion rate
* DNJ (a hypoglycemic component) contains very small amounts and has a pepsin digestion rate of around 60 %
* Steamed Mature Silkworm have no effect blood sugar decrease, It is possilble for diffuse together with 5^th-instar-3^rd silkworm(have a hypoglycemic effect) in silkworm industry
- Provide stable production of samples for health function effectiveness study
* Type of specimen: 5^th-instar-3^rdsilkworm , not steamed mature silkworm, Steamed mature silkworm
* Silkworm race: Baegokjam, Goldensilk, Yeonokjam, Redsilksilkworm
* Additionally Redsilk-silkwrom were produced and provided. The Redsilk-silkwrom breeds are very tasty, so possible to be developed as a exclusive variety.

Results
○ Paper publication(SCI): 8 papers (2 papers for SMSP generation and compenents analyses and 6 papers for functional analysis of SMSP)
○ Paper publication (non-SCI): 11 papers (3 papers for SMSP production methods, 1 paper for the component analysis of SMSP, 7 papers for functional analysis of SMSP)
○ Patent application: 7 (the prevent onset of Parkinson’s disease (PD) and liver disease and skin whitening effect, etc.)
○ Patent registration: 3 (the prevent onset of PD and liver disease and skin whitening effect)
○ Presentations in the scientific meetings: 17 (SMSP production protocols, component analysis, functional analysis, etc.)
○ Technology transfer: 4 (the prevent onset of PD and liver disease, etc.)
○ Submission of agricultural utilization agencies: 2 (SMSP production protocol etc.)
○ Standard, guideline, regulation preparation : 1
○ Farm technology road-map, consulting, onsite technical support : 4
(Transfer and support SMSP production protocols to farmers and industry)
○ Promotion score: 362point (prevention of liver disease, skin whitening, etc.)

Expected Contribution
□ Future utilization plan
○ Patent application
- Effects of steamed mature silkworm on dementia and memory improvement
- Effects of steamed mature silkworm on alopecia prevention
○ Cultivating the silkworm varieties for Steamed Mature Silkworm - 11 -
- The Redsilk-silkwrom breeds are very tasty and rich Vitamin A, so possible to be developed as a exclusive variety. (2018~)
- For cultivating the silkworm varieties for Steamed Mature Silkworm adding silkworm race rearing technique
○ Promotion
- To celebrate the world Parkinson's Day, a media promotion plan was planned around April 2019.
○ The follow-up researches on SMSPs for Healthy functional food
- Development of SMSP Golden silk breed for the prevention of dementia (BG21, 2018~ )
- Development of SMSP for skin care, Parkinson's disease, alopecia and liver disease for healthy functional food
- Additional functional capabilities proven by performing this task, such as cancer, immune-enhancing, anti-oxidation, and anti-obesity effects

□ Benefits
○ Increase the income of farmers and strengthen the industrial base by creating a new consumer market for new sericulture products, Steamed Mature Silkworm and converting them to high value-added
○ Expect strong contributions to the promotion of public health care by exploring the wide range research of health functions in Steamed Mature Silkworm required by the majority of the population.
○ The confrimation of possible prevention of Aging syndromes in super aged society by SMSPs
○ The preemptive reponse to newly emerging beauty food markets and establishment of industrialziation basis for SMSP
○ The development of alopecia treatments by SMSPs without side effects
○ The establishment of industrialization basis of SMSPs as health functional foods for detoxification of toxic chemicals

(출처 : Summary 9p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• Summary ... 9
• 목차 ... 15
• 제 1 장 연구 개발 과제의 개요 ... 16
• 제1절 연구 개발 목적 ... 16
• 제2절 연구 개발의 필요성 ... 16
• 제3절 연구 개발 범위 ... 20
• 제 2 장 국내외 기술개발 현황 ... 24
• 제1절 국내 연구 현황 ... 24
• 제2절 국외 연구 현황 ... 26
• 제 3 장 연구 수행 내용 및 결과 ... 28
• 제 4 장 목표달성도 및 관련분야 기여도 ... 180
• 제1절 : 목표대비 달성도 ... 180
• 제2절 : 정량적 성과 ... 182
• 제 5 장 연구 결과의 활용 계획 ... 183
• 제 6 장 연구 과정에서 수집한 해외 과학 기술 정보 ... 185
• 제 7 장 연구 개발 결과의 보안 등급 ... 185
• 제 8 장 국가과학기술지식정보서비스에 등록한 연구시설·장비 현황 ... 185
• 제 9 장 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 185
• 제 10 장 연구개발과제의 대표적 연구실적 ... 186
• 제 11 장 기타사항 ... 189
• 제 12 장 참고문헌 ... 189
• 끝페이지 ... 192