초록 ▼

본 연구과제에서는 천연물 라이브러리에서 후보 천연물(CINThera-1, 2, 3)을 선정하고 혼합생약추출물 (MOTILIPERM)의 정자운동 증진 효능을 확인하였고, 효능의 세포생리학적 및 분자기전을 규명하였음. 질환별 불임 동물모델 (정계정맥류·5ARI·항암제)에서 MOTILIPERM 투여에 의한 정자운동성 증가효능을 확인함. 정자 특이적 이온통로인 Hv1의 전기생리학적 특성을 확인하고, CINThera-3에 의한 칼슘증가 양상 및 정자의 형태 변화를 관찰하였음. MOTILIPERM의 임상시험용 프로토콜 및 증례기록서를 개발하여

본 연구과제에서는 천연물 라이브러리에서 후보 천연물(CINThera-1, 2, 3)을 선정하고 혼합생약추출물 (MOTILIPERM)의 정자운동 증진 효능을 확인하였고, 효능의 세포생리학적 및 분자기전을 규명하였음. 질환별 불임 동물모델 (정계정맥류·5ARI·항암제)에서 MOTILIPERM 투여에 의한 정자운동성 증가효능을 확인함. 정자 특이적 이온통로인 Hv1의 전기생리학적 특성을 확인하고, CINThera-3에 의한 칼슘증가 양상 및 정자의 형태 변화를 관찰하였음. MOTILIPERM의 임상시험용 프로토콜 및 증례기록서를 개발하여 임상시험계획 승인단계 진입을 위한 기반연구를 완성하였음

연구과정을 통해 도출된 핵심기술 및 우수한 연구결과는 SCI급 논문발표 (15편), 특허등록 (6개) 및 특허출원 (1개)의 성과를 이루었음.

(출처 : 요약서 3p)

Abstract ▼

Purpose&Contents
To develop herbal medicinal product improving sperm motility for male infertility through modulating calcium signaling pathway and to approach approval of clinical trial for new drug development

1. Research for effectiveness of botanical drug to increases sperm motility an

Purpose&Contents
To develop herbal medicinal product improving sperm motility for male infertility through modulating calcium signaling pathway and to approach approval of clinical trial for new drug development

1. Research for effectiveness of botanical drug to increases sperm motility and development of clinical trial protocol
2. Elucidation of the regulatory mechanisms of calcium signaling pathways promoting the sperm motility by natural product drug candidates
3. Structural elucidation of sperm motility inducing antioxidant natural compounds and their quantitative analysis in botanical drug candidate

Results
○ Selection of candidate natural products CINThera-1, 2, 3 from the natural product library and confirming the sperm motility enhancement effect of mixed herbal extract (MOTILIPERM)
○ CINthera-1,2,3 and MOTILIPERM treatment of varicocele-induced groups significantly increased sperm count and motility in the vas deferens and epididymis.
○ MOTILIPERM treatment of finasteride infertility animal model significantly increased sperm motility and pregnancy rate
○ Construction of electrophysiological activity evaluation system for ion channel recording in sperm
○ CINthera-3 enhancement sperm motility mechanism and action point derived Hv1 ion channel
○ Monotropein and Deacetyl aspeulosidic acid, the main compound isolated from CINthera-1, Kaempferol 3-O-glucosid amd 3,5-dicaffeoylquinic acid, the main compound isolated from CINthera-2, Quercetin 4'-O-glucoside and Quercetin, the main compound isolated from CINthera-3
○ Single active compound of MOTILIPERM treatment of varicocele-induced groups significantly increased motility
○ MOTILIPERM of 0.05 mg / ml and purified CD38 0.05 mg / ml increased sperm motility compared to the control group
○ MOTILIPERM treatment of adriamycin infertility animal model significantly increased sperm motility
○ Changes in catsper and Hv1 pathway expression by CINthera-3 and MOTILIPERM in testicular and epididymal tissues of the rats
○ As the concentration of CINthera-3 increased, the concentration of calcium in sperm increased
○ Patent registration for MOTILIPERM, determined the optimal biological dose for first-in-human study and developed the clinical study protocol

Expected Contribution
○ Development of new technology of overcoming male infertility by improving sperm motility
○ Creation of economic profit, vitalization of industry and enlargement of national interests with development of herbal medicinal product
○ Development of scientific database for a human clinical study using in vivo study data with natural products
○ Acquirement of patent and technology export for herbal medicinal product through the molecular pathogenesis studies of calcium signaling pathway
○ Enlargement to research of new drug development and discovery of rat models with disease for therapeutic target through research of mechanism of action

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 국문 요약문 ... 4
• SUMMARY ... 5
• CONTENTS ... 6
• 목차 ... 7
• 1장. 연구개발과제의 개요 ... 8
• 1절. 연구개발 목적 ... 8
• 2절. 연구개발 필요성 ... 8
• 3절. 연구개발 범위 ... 15
• 2장. 국내외 기술개발 현황 ... 16
• 3장. 연구수행 내용 및 결과 ... 21
• 제1세부과제 실적 ... 34
• 4장. 목표달성도 및 관련분야 기여도 ... 190
• 1절. 목표달성도 ... 190
• 2절. 관련분야 기여도 ... 195
• 5장. 연구결과의 활용계획 ... 197
• 6장. 연구과정에서 수집한 해외과학기술정보 ... 199
• 7장. 연구개발성과의 보안등급 ... 200
• 8장. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 200
• 9장. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 201
• 10장. 연구개발과제의 대표적 연구실적 ... 204
• 11장. 기타사항 ... 205
• 12장. 참고문헌 ... 206
• [별첨4]실적 증빙자료 ... 209
• [별첨5]기타 첨부서류 ... 210
• 끝페이지 ... 384