초록 ▼

○ 국내외 민간 식용버섯을 수집한 후 유전적 다양성 분석을 하였고, 땅찌만가닥버섯(Lyophyllum shimeji), 연기색만가닥버섯(Lyophyllum fumosum), 독청버섯아재비(Stropharia rugosoannulata)를 선발하여 톱밥 종류 및 영양원 첨가에 따른 균주생장을 확인하였음

○ 자실체 특성조사 결과 갓의 경도는 양송이버섯과 비슷하지만 대 경도는 양송이에 비해 3배이상 높았으며, 갓의 색도는 명도값이 62.4로 느타리버섯과 비슷하였음

○ 재배용 배지는 참나무:콘코브:미강:연꽃씨앗(4

○ 국내외 민간 식용버섯을 수집한 후 유전적 다양성 분석을 하였고, 땅찌만가닥버섯(Lyophyllum shimeji), 연기색만가닥버섯(Lyophyllum fumosum), 독청버섯아재비(Stropharia rugosoannulata)를 선발하여 톱밥 종류 및 영양원 첨가에 따른 균주생장을 확인하였음

○ 자실체 특성조사 결과 갓의 경도는 양송이버섯과 비슷하지만 대 경도는 양송이에 비해 3배이상 높았으며, 갓의 색도는 명도값이 62.4로 느타리버섯과 비슷하였음

○ 재배용 배지는 참나무:콘코브:미강:연꽃씨앗(40:40:10:10, v/v)을 선발하였고, 평균 수량은 121.9±26kg였음

○ 곰보버섯 재배용 배지 선발 : 종균용 배지(밀28.5%, 발효참50%, 부엽토21.5%, 백설탕5%)와 영양원 배지(밀30%, 발효참30%, 왕겨30%, 부엽토10%, 백설탕5%)의 혼합비율 설정

○ 상자재배에서 곰보버섯 재배 결과 자실체의 평균 무게는 33.2g 이였고, 노지(하우스)재배에서 평균 무게는 32.2g이였음

(출처 : 요약서 3p)

Abstract ▼

Purpose&Contents
1. The development of new species of mushrooms has increased the demand on creating new sources of income and on raising up a farm income. Also, the enlargement of ways to cultivate mushrooms has engendered the necessity of developing new small scale species, household farming-ce

Purpose&Contents
1. The development of new species of mushrooms has increased the demand on creating new sources of income and on raising up a farm income. Also, the enlargement of ways to cultivate mushrooms has engendered the necessity of developing new small scale species, household farming-centered. Therefore, the development of new mushroom species is required to meet the needs of new food ingredients, as interests for well-being food have been improved and the consumption patterns have been changed.

2. The purpose of this study was to develop farming demonstration and mass production technology by analyzing phylogeny of Tremella fuciformis, finding physiological characteristics through setting optimum culture conditions, setting the optimal medium mixing ratio of seed spawn and nutrient source, and cultivation technology according to cultivation type.

3. The purpose of this study was to develop agriculture and mass production technology by analyzing genetic taxonomic relationships of morel mushroom, by finding physiological characteristics through setting optimum cultivation conditions, by setting the optimal mixing ratio environment of culturing spawn and nutrient sources, and by developing cultivation methods according to cultivation patterns.

Results
1. In the result of investigating hyphal growth according to types of sawdust of Lyophyllum shimeji, Lyophyllum fumosum and Stropharia rugosoannulata along with the addition of nutrient, the mycelial growth of Lyophyllum shimeji and Lyophyllum fumosum was good in Medium 7 and Medium A, and that of Stropharia rugosoannulata was good in Medium B. In the result of selecting the optimal medium substances, the medium substances for Lyophyllum shimeji are carbon source(arabinose 0.7%), inorganic nitrogen(C₄H₁₂N₂O₆ 0.3%), organic nitrogen(soytone 3%), amino acid(glutamic acid 1%), organic acid(succinic acid 0.1%) and inorganic salt(CaCl₂ 2mM). In the result of bottle cultivation of Lyophyllum shimeji for cultivation technology of mushroom, cultivation took over 100 days after inoculation in 1100cc pp bottle, and the period of germination and growth was over 30 days after germ scratching work, so total period from entering the bottle to harvest was over 130 days. In the result of investing fruit body characteristics of oyster mushroom, the hardness of cap is similar to that of button mushroom, and the hardness of stem is over three times harder than that of button mushroom. The content of amino acid in Lyophyllum shimeji is 1025mg per kg, and especially the contents of Glutamic acid(92mg/kg), which is seasoning substance, and Cystein(195mg/kg), which is widely distributed in protein were high.

2. The strain, Tremella fuciformis and Annulohypoxylon stygium, used in this study was identified by ITS molecular analysis as T. fuciformis and Annulohypoxylon stygium, respectively. To define the optimum culture conditions of T. fuciformis and Annulohypoxylon stygium, we investigated effects of various nutrient sources on the fungi growth. As a result, the optimum culture conditions of T. fuciformis were 25℃, pH 5, 4.0% mannitol, 1.0% NH₄H₂PO₄, 4.0% malt extract, 1.0% glutamic acid, and 0.3% gluconic acid. The conditions for Annulohypoxylon stygium, were 30℃, pH 6, 0.1% adonitol, 1.0% NH₄H₂PO₄, 4.0% malt extract, 1.0% glutamic acid, and 0.1% gluconic acid. Cultivation medium was selected from oak: corncove: rice bran: lotus seeds (40:40:10:10, v/v). The average yield of the mushrooms was 121.9 ± 26kg, and the visual characteristics of fruiting bodies were translucent jelly, chicken crests, or tortuous flowers, and the color was pure white.

3. As the analyzed results of the genetic relationship of the collected morel mushrooms, the collected flora from oversea is categorized into Morchella septimelata and Morchella importuna, and the collected flora from domestic is categorized mostly into Morchella esculenta. The appropriate culture conditions of mushroom spawn for fine growth and development are 20~25°c temperature, 7.0~8.0 pH, and the growth and development were the best on the ground environment that contains the nutrition bases of 1~2.5% Soluble starch, 0.5~1% Yeast extract, 1mM NaCl.
The mycelial growth rate was 150mm in the mixing ratio of the first medium and the ninth medium after 11 days of culture, and the mycelial growth was the fastest. The degree of mycorrhizal formation was highest in the 7th and 8th mediums, and the medium 9th, 12th and 13th mediums showed moderate mycorrhizal formation. Mushroom cultivated in a box weighs 33.2g on average, the whole length is 133.9mm, the length of a cap is 73mm, the length of a stem is 52.2mm and mushroom cultivated outdoor and in a plastic film-house weighs 32.2g on average, the whole length is 132.7mm, the length of a cap is 63.7mm, the length of a stem is 57.0mm. The visual characteristics of the fruiting bodies were pointed ellipse with fresh shape, brown and tan in color, circular in upper part and dark brown in shape. The stipe has a thick surface, large wrinkles, smooth and light white.

Expected Contribution
We are trying to search and choose a mushroom species that enables artificial cultivation of various mushroom resources, contribute to the vitalization of the mushroom industry and increase farm income by developing a new physiology/ecosystem/cultivation technology for new mushrooms, cut down on farm management costs with low-cost and reliable mushroom production technology development, and reinforce competitiveness of domestic mushrooms by establishing a base for producing various species of mushrooms.

(출처 : Summary 7p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 5
• Summary ... 7
• 목차 ... 10
• 제 1 장 연구 개발 과제의 개요 ... 11
• 제1절 연구 개발 목적 ... 11
• 제2절 연구 개발의 필요성 ... 11
• 제3절 연구 개발 범위 ... 13
• 제 2 장 연구 수행 내용 및 결과 ... 14
• 제1절 국내외 식용가능 버섯의 재배기술 개발 및 소비가능성 검토 ... 14
• 제2절 흰목이버섯의 특성평가 및 재배기술 개발 ... 22
• 제3절 곰보버섯의 특성평가 및 재배기술 개발 ... 38
• 제 3 장 목표달성도 및 관련분야 기여도 ... 57
• 제1절 목표대비 달성도 ... 57
• 제2절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 57
• 제 4 장 연구 결과의 활용 계획 ... 58
• 제 5 장 연구 개발 결과의 보안 등급 ... 58
• 제 6 장 연구시설·장비종합정보시스템에등록한 연구시설·장비현황 ... 58
• 제 7 장 연구개발과제의 대표적 연구실적 ... 58
• 제 8 장 기타사항 ... 59
• 제 9 장 참고문헌 ... 59
• 끝페이지 ... 61