초록 ▼

□ 연구의 목적 및 내용
대사공학 및 합성 생물학의 기법을 이용한 phenolic acid유도체를 합성.
1) 4-Hydroxycoumarin합성
2) 2-hydroxybenzoyl tryptamine합성
3) Quinolone유도체 합성
3) Acridone유도체 합성

□ 연구개발성과
1) 대장균에 4-hydroxycoumarin합성 유전자 도입 및 대사 경로 설계를 통해 178.0 mg/L hydroxycoumarin합성함.
2) 2-hydrobenzoyl tryptamine 합

□ 연구의 목적 및 내용
대사공학 및 합성 생물학의 기법을 이용한 phenolic acid유도체를 합성.
1) 4-Hydroxycoumarin합성
2) 2-hydroxybenzoyl tryptamine합성
3) Quinolone유도체 합성
3) Acridone유도체 합성

□ 연구개발성과
1) 대장균에 4-hydroxycoumarin합성 유전자 도입 및 대사 경로 설계를 통해 178.0 mg/L hydroxycoumarin합성함.
2) 2-hydrobenzoyl tryptamine 합성에 필요한 유전자의 클로닝 및 대장균에 도입을 통해 227.8 mg/L의 2-hydroxybenzoyl tryptamine 합성함.
3) Quinolone 및 N-metyl quinolone합성에 필요한 유전자들을 클로닝한 후 대장균에 도입하고 대사공학 기법을 이용하여 이들 두 물질의 기질인 anthranilate의 합성량을 증가 시켜서 735.7 mg/L quinoline과 17.5 mg/L의 N-methyl quinolone을 합성함.
4) Acridone 및 N-methyl acridone 합성에 필요한 유전자를 대장균에 도입하고 이 두 물질의 기질의 합성을 최적화하여 17.3 mg/L의 acridone과 26.0 mg/L의 N-methyl acridone을 합성함.

□ 연구개발성과의 활용계획 (기대효과)
1) 후속 연구로 acridone유도체에 구조를 변형하는 연구가 진행중임.
2) 기술이전을 논의 중에 있음.

(출처 : 국문 요약문 4p)

Abstract ▼

□ Purpose&Contents
Synthesis of phenolic acid derivatives using metabolic engieering and synthetic biology in Escherichia coli
1) Synthesis of 4-hydroxycoumarin
2) Synthesis of 2-hydroxybenzoyl tryptamine
3) Synthesis of quinolone derivatives
3) Synthesis of acridone derivatives

□ Purpose&Contents
Synthesis of phenolic acid derivatives using metabolic engieering and synthetic biology in Escherichia coli
1) Synthesis of 4-hydroxycoumarin
2) Synthesis of 2-hydroxybenzoyl tryptamine
3) Synthesis of quinolone derivatives
3) Synthesis of acridone derivatives

□ Results
1) By introducing 4-hydroxycoumarin synthesis genes and engineering metabolic pathway of E. coli, 178.0 mg/L hydroxycoumarin was synthesized.
2) 227.8 mg/L 2-hydroxybenzoyl tryptamine was synthesized by cloning 2-hydroxybenzoyl tryptamine synthesis genes and introducing them into E. coli.
3) Through cloning and constructing quinolone and N-metyl quinolone synthesis genes, and engineering E. coli metabolic pathway 735.7 mg/L quinoline and 17.5 mg/L의 N-methyl quinolone were synthesized, respectively.
4) 17.3 mg/L acridone and 26.0 mg/L의 N-methyl acridone were synthesized by introducting acridone and N-methyl acridone synthesis constructs and engineering metabolic pathway of E. coli.

□ Expected Contribution
1) Synthesis of acridone derivatives is under wasy.
2) Transfer of current experimental result into a company is under wasy.

(출처 : SUMMARY 5p)