초록 ▼

Ⅳ. 연구개발결과
○ Autolysin 생산 및 수확 조건을 확립하였고, 이를 활용하여 cell permeability를 개선하여 CRISPR/Cas9 적용 효율을 개선하였음
○ 또한, 다양한 효소를 이용한 cell permeability 개선 연구 결과 lysozyme을 이용하여 효과적으로 활용하였음
○ 결과적으로 MAA7 유전자를 기존 문헌 대비 1370% 증대된 효율로 knock out하는데 성공하였음 (형질전환 효율 : 8.9×10^-8 → 1.2×10^-6)
○ 유용

Ⅳ. 연구개발결과
○ Autolysin 생산 및 수확 조건을 확립하였고, 이를 활용하여 cell permeability를 개선하여 CRISPR/Cas9 적용 효율을 개선하였음
○ 또한, 다양한 효소를 이용한 cell permeability 개선 연구 결과 lysozyme을 이용하여 효과적으로 활용하였음
○ 결과적으로 MAA7 유전자를 기존 문헌 대비 1370% 증대된 효율로 knock out하는데 성공하였음 (형질전환 효율 : 8.9×10^-8 → 1.2×10^-6)
○ 유용 단백질인 sfGFP와 PETase 유전자의 삽입을 PCR 분석을 통해 확인하였고 RT-qPCR, western blot, 형광분석 등을 통해 sfGFP의 발현을 일부 확인하였음


(출처 : 요약문 4p)

Abstract ▼

Ⅳ. Results
- Gene stability was confirmed with the long-term cryopreservation.
- It was confirmed that the original cultivation conditions could be applicable for transformant.
- Demonstration of industrial-scale production with 5-ton-scale bioreactor is being progressed and it would be fin

Ⅳ. Results
- Gene stability was confirmed with the long-term cryopreservation.
- It was confirmed that the original cultivation conditions could be applicable for transformant.
- Demonstration of industrial-scale production with 5-ton-scale bioreactor is being progressed and it would be finished in the research period.
- From the large-scale fermentation of transformant, over than 30% enhanced production yield of MEL was observed, and the quality of the product is also validated.


(source: SUMMARY 5p)