초록

AI-Helper

이상의 결과를 요약하면 다음과 같다. 건조된 혈액에서 직접 추출한 DNA양은 7∼9㎍/㎖로써 혈액에서 직접 추출한 DNA양 (22㎍/㎖)에 비해 1/3의 양이었다. 건조된 혈액에서 추출한 DNA는 DNA분석을 위해 충분한 양으로서 pERT 87-8 probe/Taq 1로써 hybridize한 결과 3.6 kb, 0.9 kb 에 band 가 나타났다....

이상의 결과를 요약하면 다음과 같다. 건조된 혈액에서 직접 추출한 DNA양은 7∼9㎍/㎖로써 혈액에서 직접 추출한 DNA양 (22㎍/㎖)에 비해 1/3의 양이었다. 건조된 혈액에서 추출한 DNA는 DNA분석을 위해 충분한 양으로서 pERT 87-8 probe/Taq 1로써 hybridize한 결과 3.6 kb, 0.9 kb 에 band 가 나타났다.

Abstract ▼ AI-Helper

Microextraction of DNA from dried blood specimens would ease specimen transport to centralized laboratory facilities for recombinant DNA diagnosis in the same manner as use of dried blood spots allowed the broad application of screening tests to newborn populations. These dried blood samples have be...

Microextraction of DNA from dried blood specimens would ease specimen transport to centralized laboratory facilities for recombinant DNA diagnosis in the same manner as use of dried blood spots allowed the broad application of screening tests to newborn populations. These dried blood samples have been used for various tests including those based on screening for red blood cell proteins, as in galactosemia or the hemoglobinopathies, for hormone metabolite accumulation, as in phenylketonuria, homocystin uria, or maple syrup urine disease. I have been interested in appling molecular genetics techniques to newborn screening. Thus, the purpose of this study was to determine whether DNA was present in dried blood spots on filter paper blotters in a form that could be extracted and isolated and in sufficient quantities for use in DNA analyses. The suitable results were obtained as follows: The extracted volume of DNA was 7-9μg per one blotted blood paper. There was no differentiation of extracted DNA volume among dry period. This DNA volume was about 1/3 of direct ordinary extracts of 1 ml whole blood. This microextraction procedure will allow immediate application of molecular genetic technology to direct newborn screning follow up of disorders amenable to DNA diagnosis.

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