Various steps, from preparation of TLC plate to color reaction of spots, of urinary amino acids by means of thin layer chromatography(TLC) were studied. Based upon this procedure studied by Authors, Rf values of 39 ninhydrin positive substances which appeared frequently in urine were measured in acc...
Various steps, from preparation of TLC plate to color reaction of spots, of urinary amino acids by means of thin layer chromatography(TLC) were studied. Based upon this procedure studied by Authors, Rf values of 39 ninhydrin positive substances which appeared frequently in urine were measured in accordance with three different kinds of solvent systems, i. e., chloroform-methanol-17% ammonia, n-butanol-glacial acetic acid-water, and phenol-water. In quantitaion of urinary amino acids unique Author's method was introduced as figure 4. Urinary amino acids in Korean, 10 amino acids, in accordance with the order of high frequency, glycine, histidine, aIanine, serine, taurine, citrulline, β-aminoisobutyric acid, arginine, valine, and hydroxyproline, were amount to 82.3% of total amino acids appeared in urine. The remaining 14 urinary amino acids were taken as 17.7%. Comparison with values from Seoul and Kwangju areas obtained on urinary amino acids, former were 7.3 amino acids per individual and range of 4-11 amino acid, and latter were 4.0 and 3-7 amino acids. In sorts of amino acids alanine, taurine, serine, and citrulline were taken as high frequency in Seoul area but were low in Kwangju area. However hydroxyproline and asparagine were appeared as reverse frequency. By studies depending upon morning, noon, and evening groups, variation of urinary amino acids were not showed markedly. Urinary amino acids arginine and valine were appeared scarecely in evening but β-aminoisobutyric acid and tyrosine were frequently.
Various steps, from preparation of TLC plate to color reaction of spots, of urinary amino acids by means of thin layer chromatography(TLC) were studied. Based upon this procedure studied by Authors, Rf values of 39 ninhydrin positive substances which appeared frequently in urine were measured in accordance with three different kinds of solvent systems, i. e., chloroform-methanol-17% ammonia, n-butanol-glacial acetic acid-water, and phenol-water. In quantitaion of urinary amino acids unique Author's method was introduced as figure 4. Urinary amino acids in Korean, 10 amino acids, in accordance with the order of high frequency, glycine, histidine, aIanine, serine, taurine, citrulline, β-aminoisobutyric acid, arginine, valine, and hydroxyproline, were amount to 82.3% of total amino acids appeared in urine. The remaining 14 urinary amino acids were taken as 17.7%. Comparison with values from Seoul and Kwangju areas obtained on urinary amino acids, former were 7.3 amino acids per individual and range of 4-11 amino acid, and latter were 4.0 and 3-7 amino acids. In sorts of amino acids alanine, taurine, serine, and citrulline were taken as high frequency in Seoul area but were low in Kwangju area. However hydroxyproline and asparagine were appeared as reverse frequency. By studies depending upon morning, noon, and evening groups, variation of urinary amino acids were not showed markedly. Urinary amino acids arginine and valine were appeared scarecely in evening but β-aminoisobutyric acid and tyrosine were frequently.
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