Abstract ▼ AI-Helper

The use of 2-bromoacetyl-6-methoxynaphthalene as a fluorogenic labelling reagent in precolumn derivatization for the HPLC separation of carboxylic acids was investigated. 2-bromoacetyl-6-methoxynaphthalene reacted with pranoprofen, 2-arylpropionic acid, a group of non-steroidal anti-inflammatory dru...

The use of 2-bromoacetyl-6-methoxynaphthalene as a fluorogenic labelling reagent in precolumn derivatization for the HPLC separation of carboxylic acids was investigated. 2-bromoacetyl-6-methoxynaphthalene reacted with pranoprofen, 2-arylpropionic acid, a group of non-steroidal anti-inflammatory drugs (NSAIDs) in the presence of triethylamine. To achieve optimum conditions the effect of temperature, reagent, triethylamine and Br-AMN molar ratio on the course of reaction was investigated. The compound reacted (40℃, at 60min) with carboxylic acid to give fluorescent esters that can be separated by reversed phase HPLC and detected at λex. 370.2nm, λem. 430.6nm. In the developed HPLC-fluorescence method, the limits of detection of the pranoprofen were 5ng/ml based on the signal-to-noise(S/N) ratio of 3 and the limits of quantification were 50ng/ml based S/N ratio of 10. Recovery of standard in test solution ranged from 98.65∼101.95%. The reproducibility of the measurement between within-day-run was showed to be very good, with precision typically below 3% R.S.D at the ㎍/ml level concentration. Application to the determination of pranoprofen in pharmaceutical formulations are described. The assay result of pranoprofen in pharmaceuticals revealed that pranoprofen was detected in A(104.69%), B(102.08%), C(106.24%), D(98.79%). The proposed method proved to be applicable to the quantitation of carboxylic acids with satisfactory reliability and sensitivity.

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