콩의 품질 및 영양가치를 떨어지게 하는 성분인 Lipoxygenase와 Kunitz trypsin inhibitor 단백질이 유전적으로 결핍되어 있고 소화 장애를 일으키는 Raffinose 및 Stachyose의 함량이 낮은 콩 계통을 육성하기 위하여 “진품콩2호” x PI200508 및 PI200508 x “개척2호”의 두 육종집단을 얻은 후 수행된 본 실험에서 얻어진 결과를 요약하면 다음과 같다. 1. 두 집단으로부터 Raffinos...
콩의 품질 및 영양가치를 떨어지게 하는 성분인 Lipoxygenase와 Kunitz trypsin inhibitor 단백질이 유전적으로 결핍되어 있고 소화 장애를 일으키는 Raffinose 및 Stachyose의 함량이 낮은 콩 계통을 육성하기 위하여 “진품콩2호” x PI200508 및 PI200508 x “개척2호”의 두 육종집단을 얻은 후 수행된 본 실험에서 얻어진 결과를 요약하면 다음과 같다. 1. 두 집단으로부터 Raffinose 및 Stachyose의 함량이 낮은 것으로 선 발된 계통은 다음 세대에서 Raffinose 및 Stachyose의 함량이 낮아 이들 두 성분은 환경보다는 유전적인 영향을 많이 받는 것으로 나 타났다. 2. Lipoxygenase 1, 2, 3이 결핍되어 있고 Raffinose와 Stachyose의 함 량이 낮은 F4 26계통이 선발되었다. 3. Lipoxygenase 1, 2, 3 또는 2, 3 및 Kunitz trypsin inhibitor 단백질 이 결핍되어 있고 Raffinose와 Stachyose의 함량이 낮은 F3 5계통 이 선발되었다. 4. Lipoxygenase 1, 2, 3이 결핍되어 있고 Raffinose와 Stachyose의 함 량이 낮은 두 계통의 포장 평가 결과 장려품종과 비교하여 개화기, 성숙기, 초장, 초형, 도복, 수량, 병해충 저항성등에서 비슷하여 품종 육성이 가능함을 제시 하였다.
콩의 품질 및 영양가치를 떨어지게 하는 성분인 Lipoxygenase와 Kunitz trypsin inhibitor 단백질이 유전적으로 결핍되어 있고 소화 장애를 일으키는 Raffinose 및 Stachyose의 함량이 낮은 콩 계통을 육성하기 위하여 “진품콩2호” x PI200508 및 PI200508 x “개척2호”의 두 육종집단을 얻은 후 수행된 본 실험에서 얻어진 결과를 요약하면 다음과 같다. 1. 두 집단으로부터 Raffinose 및 Stachyose의 함량이 낮은 것으로 선 발된 계통은 다음 세대에서 Raffinose 및 Stachyose의 함량이 낮아 이들 두 성분은 환경보다는 유전적인 영향을 많이 받는 것으로 나 타났다. 2. Lipoxygenase 1, 2, 3이 결핍되어 있고 Raffinose와 Stachyose의 함 량이 낮은 F4 26계통이 선발되었다. 3. Lipoxygenase 1, 2, 3 또는 2, 3 및 Kunitz trypsin inhibitor 단백질 이 결핍되어 있고 Raffinose와 Stachyose의 함량이 낮은 F3 5계통 이 선발되었다. 4. Lipoxygenase 1, 2, 3이 결핍되어 있고 Raffinose와 Stachyose의 함 량이 낮은 두 계통의 포장 평가 결과 장려품종과 비교하여 개화기, 성숙기, 초장, 초형, 도복, 수량, 병해충 저항성등에서 비슷하여 품종 육성이 가능함을 제시 하였다.
Soybean [Glycine max (L.) Merr.] seed is the main source of protein and oil for human and animal. Lipoxygenase and Kunitz trypsin inhibitor (KTI) protein of mature soybean seed are main anti-nutritional factors in soybean seed. Also, raffinose and stachyose are considered anti-nutritional units beca...
Soybean [Glycine max (L.) Merr.] seed is the main source of protein and oil for human and animal. Lipoxygenase and Kunitz trypsin inhibitor (KTI) protein of mature soybean seed are main anti-nutritional factors in soybean seed. Also, raffinose and stachyose are considered anti-nutritional units because they cannot be digested due to the lack of α-galactosidase activity in the gut of monogastric animals. The objectives of this research were to breed a new strains with lipoxygenase-free and low raffinose and stachyose content and were to improve a strain with lipoxygenase and KTI-free and low raffinose and stachyose content. Selection effect for low raffinose and stachyose content was observed in two generation. Also, agronomic traits for new strains with lipoxygenase-free and low raffinose and stachyose content were observed in field. Two populations (“Jinpumkong2ho" x PI200508 and PI200508 x “Gaechuck2ho”) were developed. Presence and electrophoresis by using seeds. Content of raffinose and stachyose was detected by HPLC. Across generation, raffinose and stachyose content was very similar. Twenty six F4 plants with lipoxygenase 1,2,3-free and low raffinose and stachyose content were selected. Three F3 plants with lipoxygenase 1,2,3 and KTI-free and low raffinose and stachyose content were selected. Two F3 plants with lipoxygenase 2,3 and KTI-free and low raffinose and stachyose content were selected. Seed coat color and hilum color of two strains were yellow. Two strains with lipoxygenase 1,2,3-free and low raffinose and stachyose content were flowered on July 30 and were matured on Ocober 1. Plant height of two strains was about 70 cm. The 100-seed weight of two strains was about 20g. Yield of two strains was about 330 kg/10a in 2009 field.
Soybean [Glycine max (L.) Merr.] seed is the main source of protein and oil for human and animal. Lipoxygenase and Kunitz trypsin inhibitor (KTI) protein of mature soybean seed are main anti-nutritional factors in soybean seed. Also, raffinose and stachyose are considered anti-nutritional units because they cannot be digested due to the lack of α-galactosidase activity in the gut of monogastric animals. The objectives of this research were to breed a new strains with lipoxygenase-free and low raffinose and stachyose content and were to improve a strain with lipoxygenase and KTI-free and low raffinose and stachyose content. Selection effect for low raffinose and stachyose content was observed in two generation. Also, agronomic traits for new strains with lipoxygenase-free and low raffinose and stachyose content were observed in field. Two populations (“Jinpumkong2ho" x PI200508 and PI200508 x “Gaechuck2ho”) were developed. Presence and electrophoresis by using seeds. Content of raffinose and stachyose was detected by HPLC. Across generation, raffinose and stachyose content was very similar. Twenty six F4 plants with lipoxygenase 1,2,3-free and low raffinose and stachyose content were selected. Three F3 plants with lipoxygenase 1,2,3 and KTI-free and low raffinose and stachyose content were selected. Two F3 plants with lipoxygenase 2,3 and KTI-free and low raffinose and stachyose content were selected. Seed coat color and hilum color of two strains were yellow. Two strains with lipoxygenase 1,2,3-free and low raffinose and stachyose content were flowered on July 30 and were matured on Ocober 1. Plant height of two strains was about 70 cm. The 100-seed weight of two strains was about 20g. Yield of two strains was about 330 kg/10a in 2009 field.
주제어
#Lipoxygenase Raffinose 및 Stachyose 콩 SDS-PAGE HPLC
학위논문 정보
저자
성미경
학위수여기관
경상대학교 대학원
학위구분
국내석사
학과
응용생명과학부 농학 전공
지도교수
정종일
발행연도
2010
총페이지
ⅴ, 25 p.
키워드
Lipoxygenase Raffinose 및 Stachyose 콩 SDS-PAGE HPLC
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