효모를 이용한 에탄올 발효 시 에탄올 생산성을 증가시키기 위해 공기첨가 효과를 에탄올 발효 효모인 Saccharomyces cerevisiae SC 1024와 Saccharomyces cerevisiae ATCC 24858을 이용하여 조사 하였다. 먼저, 두 균주의 성장과 ...
효모를 이용한 에탄올 발효 시 에탄올 생산성을 증가시키기 위해 공기첨가 효과를 에탄올 발효 효모인 Saccharomyces cerevisiae SC 1024와 Saccharomyces cerevisiae ATCC 24858을 이용하여 조사 하였다. 먼저, 두 균주의 성장과 glucose 소비 그리고 에탄올 내성을 확인하였더니 Saccharomyces cerevisiae SC 1024 균주가 성장과 glucose 소비 측면에서 Saccharomyces cerevisiae ATCC 24858 균주 보다 더 우수함을 확인하였다. 특히 에탄올 100 g/L에서는 Saccharomyces cerevisiae SC 1024가 상대적으로 Saccharomyces cerevisiae ATCC 24858보다 inhibition을 덜 받는 다는 것을 알 수 있었다. 본 연구에서는 산업적으로 응용 가능한 유가식 배양 공정을 만들기 위하여 산업용으로 사용되는 성분을 에탄올 발효에 사용하였다. 유가식 ethanol 발효 시 공기 첨가 효과를 조사하기 위하여 0, 0.13, 0.33, 0.8 vvm의 공기를 공급하였고, non-sterile glucose powder를 직접 feeding 하였다. 이때 에탄올 productivity 측면에서 유가식 에탄올 발효 동안 aeration rate이 좋은 효과를 나타낸다는 것을 보여주었으며, 특히 0.33 vvm에서 에탄올 생산성이 최고임을 확인하였다. 또한 에탄올 발효 시 공기 첨가가 glycerol 생산을 감소시켜 에탄올 생산을 증가시키는 것을 알았다. 공기를 첨가하는 장기간에 걸친 반복 유가식 에탄올 발효에서 에탄올 발효의 공정 안정성을 검증하기 위하여 396 h 동안 유가식 발효를 수행하였다. 이때, 안정적으로 에탄올 생산성이 증가하는 것을 확인하였다. 한편, Saccharomyces cerevisiae ATCC 24858 균주를 사용한 유가식 에탄올 발효 공정에서는 Saccharomyces cerevisiae 1024보다 더 짧은 시간 내에 반복적으로 배지를 교체 하였을 때, 더 좋은 에탄올 생산성을 보이는 것을 확인하였다. 결론적으로 본 연구에서는 최적의 aeration 조건에서 glucose와 배지를 어떠한 전략으로 withdrawal-fill 해야 에탄올의 생산이 최대로 증가할 수 있는지에 대하여 연구하였으며, 이러한 연구를 통하여 반복 유가식 에탄올 발효에서 최고의 에탄올 생산을 위하여서는 운전전략의 설계와 실증이 중요하다는 것을 보여주었으며, 다른 발효 생산물 반복 유가식 발효 방법으로도 응용 되어 질 수 있을 것으로 생각된다.
효모를 이용한 에탄올 발효 시 에탄올 생산성을 증가시키기 위해 공기첨가 효과를 에탄올 발효 효모인 Saccharomyces cerevisiae SC 1024와 Saccharomyces cerevisiae ATCC 24858을 이용하여 조사 하였다. 먼저, 두 균주의 성장과 glucose 소비 그리고 에탄올 내성을 확인하였더니 Saccharomyces cerevisiae SC 1024 균주가 성장과 glucose 소비 측면에서 Saccharomyces cerevisiae ATCC 24858 균주 보다 더 우수함을 확인하였다. 특히 에탄올 100 g/L에서는 Saccharomyces cerevisiae SC 1024가 상대적으로 Saccharomyces cerevisiae ATCC 24858보다 inhibition을 덜 받는 다는 것을 알 수 있었다. 본 연구에서는 산업적으로 응용 가능한 유가식 배양 공정을 만들기 위하여 산업용으로 사용되는 성분을 에탄올 발효에 사용하였다. 유가식 ethanol 발효 시 공기 첨가 효과를 조사하기 위하여 0, 0.13, 0.33, 0.8 vvm의 공기를 공급하였고, non-sterile glucose powder를 직접 feeding 하였다. 이때 에탄올 productivity 측면에서 유가식 에탄올 발효 동안 aeration rate이 좋은 효과를 나타낸다는 것을 보여주었으며, 특히 0.33 vvm에서 에탄올 생산성이 최고임을 확인하였다. 또한 에탄올 발효 시 공기 첨가가 glycerol 생산을 감소시켜 에탄올 생산을 증가시키는 것을 알았다. 공기를 첨가하는 장기간에 걸친 반복 유가식 에탄올 발효에서 에탄올 발효의 공정 안정성을 검증하기 위하여 396 h 동안 유가식 발효를 수행하였다. 이때, 안정적으로 에탄올 생산성이 증가하는 것을 확인하였다. 한편, Saccharomyces cerevisiae ATCC 24858 균주를 사용한 유가식 에탄올 발효 공정에서는 Saccharomyces cerevisiae 1024보다 더 짧은 시간 내에 반복적으로 배지를 교체 하였을 때, 더 좋은 에탄올 생산성을 보이는 것을 확인하였다. 결론적으로 본 연구에서는 최적의 aeration 조건에서 glucose와 배지를 어떠한 전략으로 withdrawal-fill 해야 에탄올의 생산이 최대로 증가할 수 있는지에 대하여 연구하였으며, 이러한 연구를 통하여 반복 유가식 에탄올 발효에서 최고의 에탄올 생산을 위하여서는 운전전략의 설계와 실증이 중요하다는 것을 보여주었으며, 다른 발효 생산물 반복 유가식 발효 방법으로도 응용 되어 질 수 있을 것으로 생각된다.
In this study, we utilized a unique strategy for the fed-batch fermentation process using ethanol-tolerant Saccharomyces cerevisiae to achieve a high-level of ethanol production that could be practically applied to industrial ethanol production. During this study, the aeration rate was controlled at...
In this study, we utilized a unique strategy for the fed-batch fermentation process using ethanol-tolerant Saccharomyces cerevisiae to achieve a high-level of ethanol production that could be practically applied to industrial ethanol production. During this study, the aeration rate was controlled at 0.0, 0.13, 0.33, and 0.8 vvm to determine the optimal aeration conditions for the production of ethanol. When aeration was conducted, the ethanol production and productivity were superior to those when the aeration was not conducted. In addition, we investigated the effects of aeration on ethanol inhibition and glycerol production during fed-batch ethanol fermentation. Specifically, when aeration was conducted, the glycerol yield and specific glycerol production rate decreased approximately 50 and 70%, respectively. We indicated that aeration during fed-batch ethanol fermentation may improve the ethanol concentration in the final culture broth, as well as the ethanol productivity. We attempted to verify the process stability of long-term fed-batch ethanol fermentation, where we compared the data when the aeration was carried out (0.33 vvm) or not, and we carried out the long-term repeated fed-batch operation for 396 h, mimicking a long-term industrial bioethanol production process. During 396 h, ethanol was cumulatively produced 1908.9 g, which was increased by 12.47%, when the aeration was carried out, compared with 1697.2 g when the aeration was not carried out. It was definitely deduced that the reason was that the cell growth was greatly improved by overcoming the ethanol inhibition and the glycerol production remarkably decreased when the aeration was carried out, although aeration in ethanol fermentation decreased the specific ethanol production rate and ethanol yield. mean while, We designed the optimal operational strategy in repeated fed-batch ethanol fermentation using Sacchromyces cerevisiae ATCC 24858 in views of ethanol yield, specific ethanol production rate, and ethanol productivity, when the aeration rate were controlled at 0.0 and 0.33 vvm. Coincidentally, the time intervals of withdrawal-fill of culture medium (24 and 36 h) were investigated. Ethanol yield and ethanol productivity when the aeration was carried out at 0.33 vvm were superior to those when the aeration was not carried out. Additionally, those parameters when the time interval of withdrawal-fill of culture medium were 24 h was superior to those when time interval of withdrawal-fill of culture medium was 36 h. The total ethanol production reached at the greatest value, 703.8 g-ethanol, when the aeration was carried out at 0.33 vvm and the time interval of withdrawal-fill of culture medium was 24 h. In this study, we verified experimentally the necessity of designing the operational strategy for increasing ethanol production in terms of aeration rate and time interval of withdrawal-fill of culture medium in the repeated fed-batch ethanol fermentation.
In this study, we utilized a unique strategy for the fed-batch fermentation process using ethanol-tolerant Saccharomyces cerevisiae to achieve a high-level of ethanol production that could be practically applied to industrial ethanol production. During this study, the aeration rate was controlled at 0.0, 0.13, 0.33, and 0.8 vvm to determine the optimal aeration conditions for the production of ethanol. When aeration was conducted, the ethanol production and productivity were superior to those when the aeration was not conducted. In addition, we investigated the effects of aeration on ethanol inhibition and glycerol production during fed-batch ethanol fermentation. Specifically, when aeration was conducted, the glycerol yield and specific glycerol production rate decreased approximately 50 and 70%, respectively. We indicated that aeration during fed-batch ethanol fermentation may improve the ethanol concentration in the final culture broth, as well as the ethanol productivity. We attempted to verify the process stability of long-term fed-batch ethanol fermentation, where we compared the data when the aeration was carried out (0.33 vvm) or not, and we carried out the long-term repeated fed-batch operation for 396 h, mimicking a long-term industrial bioethanol production process. During 396 h, ethanol was cumulatively produced 1908.9 g, which was increased by 12.47%, when the aeration was carried out, compared with 1697.2 g when the aeration was not carried out. It was definitely deduced that the reason was that the cell growth was greatly improved by overcoming the ethanol inhibition and the glycerol production remarkably decreased when the aeration was carried out, although aeration in ethanol fermentation decreased the specific ethanol production rate and ethanol yield. mean while, We designed the optimal operational strategy in repeated fed-batch ethanol fermentation using Sacchromyces cerevisiae ATCC 24858 in views of ethanol yield, specific ethanol production rate, and ethanol productivity, when the aeration rate were controlled at 0.0 and 0.33 vvm. Coincidentally, the time intervals of withdrawal-fill of culture medium (24 and 36 h) were investigated. Ethanol yield and ethanol productivity when the aeration was carried out at 0.33 vvm were superior to those when the aeration was not carried out. Additionally, those parameters when the time interval of withdrawal-fill of culture medium were 24 h was superior to those when time interval of withdrawal-fill of culture medium was 36 h. The total ethanol production reached at the greatest value, 703.8 g-ethanol, when the aeration was carried out at 0.33 vvm and the time interval of withdrawal-fill of culture medium was 24 h. In this study, we verified experimentally the necessity of designing the operational strategy for increasing ethanol production in terms of aeration rate and time interval of withdrawal-fill of culture medium in the repeated fed-batch ethanol fermentation.
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