This study was conducted to investigate the antimicrobial activity of Ginkgo biloba L. leaves, seed and outer seedcoat against Bacillus cereus(B. cereus), Bacillus subtilis(B. subtilis), Escherichia coli(E. coli), Klebsiella pneumoniae(K. pneumoniae), Listeria monocytogenes(L. mono- cytogenes), Salm...
This study was conducted to investigate the antimicrobial activity of Ginkgo biloba L. leaves, seed and outer seedcoat against Bacillus cereus(B. cereus), Bacillus subtilis(B. subtilis), Escherichia coli(E. coli), Klebsiella pneumoniae(K. pneumoniae), Listeria monocytogenes(L. mono- cytogenes), Salmonella typhimurium(Sal. typhimurium), Staphylococcus aureus (S. aureus), Yersinia enterocolitica(Y. enterocolitica). Antimicrobial effect of Ginkgo biloba L. leaves(GBL), seed(GBS) and outer seedcoat(GBO) was examined by paper disc method, optical density method and determine minimum inhibition concentraion(MIC) and observed by scanning electron microscope(SEM) to figure out the morphological change on the surface when Ginkgo biloba leaves extract was treated. The extracts of GBL, GBS and GBO was extracted by solvent such as methanol(MeOH ex.), ethanol(EtOH ex.), water(Water ex.) and the MeOH ex. of GBL, GBO showed the highest antimicrobial activity against B. cereus, B. subtilis, K. pneumoniae, L. monocytogenes, Sal. typhimurium, S. aureus, Y. enterocolitica excepted E. coli and thus was further fractionated. The MICs of the chloroform fraction of GBL MeOH ex. were 125 μg/mL against B. subtilis, L. monocytogenes and 500 μg/mL against B. cereus, S. aureus and 1000 μg/mL against K. pneumoniae, Sal. typhimurium, Y. enterocolitica. The MICs of the chloroform fraction of GBO MeOH ex. were 62.5 μg/mL against B. cereus and 125 μg/mL against B. subtilis, L. monocytogenes and 500 μg/mL against K. pneumoniae, S. aureus, Y. enterocolitica and 1000 μg/mL against Sal. typhimurium. Among these fractions, the chloroform fraction of GBO MeOH ex. showed the strongest antimicrobial activities against microorganisms excepted E. coli. GBO MeOH ex. showed the higher antimicrobial activitiy than GBL MeOH ex. All GBS solvent extracts and GBL, GBO Water ex. did not show any antimicrobial activity against microorganisms. The microorganisms treated with chloroform extracts(2000 μg/mL) of GBL MeOH ex. and GBO MeOH ex. was observed by scanning electron microscope(SEM). The cells were expanded and a part of cell wall was completely destructed by GBL and GBO. The results in the present study demonstrate antimicrobial effects of GBL and GBO methanol extracts against microorganisms, suggesting that Ginkgo biloba L. could be further developed into a natural antimicrobial agent.
This study was conducted to investigate the antimicrobial activity of Ginkgo biloba L. leaves, seed and outer seedcoat against Bacillus cereus(B. cereus), Bacillus subtilis(B. subtilis), Escherichia coli(E. coli), Klebsiella pneumoniae(K. pneumoniae), Listeria monocytogenes(L. mono- cytogenes), Salmonella typhimurium(Sal. typhimurium), Staphylococcus aureus (S. aureus), Yersinia enterocolitica(Y. enterocolitica). Antimicrobial effect of Ginkgo biloba L. leaves(GBL), seed(GBS) and outer seedcoat(GBO) was examined by paper disc method, optical density method and determine minimum inhibition concentraion(MIC) and observed by scanning electron microscope(SEM) to figure out the morphological change on the surface when Ginkgo biloba leaves extract was treated. The extracts of GBL, GBS and GBO was extracted by solvent such as methanol(MeOH ex.), ethanol(EtOH ex.), water(Water ex.) and the MeOH ex. of GBL, GBO showed the highest antimicrobial activity against B. cereus, B. subtilis, K. pneumoniae, L. monocytogenes, Sal. typhimurium, S. aureus, Y. enterocolitica excepted E. coli and thus was further fractionated. The MICs of the chloroform fraction of GBL MeOH ex. were 125 μg/mL against B. subtilis, L. monocytogenes and 500 μg/mL against B. cereus, S. aureus and 1000 μg/mL against K. pneumoniae, Sal. typhimurium, Y. enterocolitica. The MICs of the chloroform fraction of GBO MeOH ex. were 62.5 μg/mL against B. cereus and 125 μg/mL against B. subtilis, L. monocytogenes and 500 μg/mL against K. pneumoniae, S. aureus, Y. enterocolitica and 1000 μg/mL against Sal. typhimurium. Among these fractions, the chloroform fraction of GBO MeOH ex. showed the strongest antimicrobial activities against microorganisms excepted E. coli. GBO MeOH ex. showed the higher antimicrobial activitiy than GBL MeOH ex. All GBS solvent extracts and GBL, GBO Water ex. did not show any antimicrobial activity against microorganisms. The microorganisms treated with chloroform extracts(2000 μg/mL) of GBL MeOH ex. and GBO MeOH ex. was observed by scanning electron microscope(SEM). The cells were expanded and a part of cell wall was completely destructed by GBL and GBO. The results in the present study demonstrate antimicrobial effects of GBL and GBO methanol extracts against microorganisms, suggesting that Ginkgo biloba L. could be further developed into a natural antimicrobial agent.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.