요 약 상황버섯은 약용버섯으로 소나무 비늘버섯과에 속하는 다양한 질병의 치료를 위해 전통 의학으로 사용 되어져 왔습니다. 본 연구에서는 상황버섯 균주 배양액의 화학적 구성성분과 생물활성을 연구하였다. 상황버섯 균주를 PDB 배지를 이용하여 배양하였고, 이 배양액을 ethyl acetate로 추출하였다. Ethyl acetate 분획물을 감압농축 한 후, ODS column chromatography와 Sephadex LH-20 column chromatography를 차례로 분리 정제 실험을 실시하였다. 최종적으로 ...
요 약 상황버섯은 약용버섯으로 소나무 비늘버섯과에 속하는 다양한 질병의 치료를 위해 전통 의학으로 사용 되어져 왔습니다. 본 연구에서는 상황버섯 균주 배양액의 화학적 구성성분과 생물활성을 연구하였다. 상황버섯 균주를 PDB 배지를 이용하여 배양하였고, 이 배양액을 ethyl acetate로 추출하였다. Ethyl acetate 분획물을 감압농축 한 후, ODS column chromatography와 Sephadex LH-20 column chromatography를 차례로 분리 정제 실험을 실시하였다. 최종적으로 Caffeic acid (1), inotilone (2), 4-(3,4-dihydroxyphenyl) -3-buten-2-one (3), phellinane H (4), (2E,4E)-(+)-4′-Hydroxy-α-ionylideneacetic acid (5) and (2E,4E)-α-ionylideneacetic acid (6) 6종의 화합물이 분리되었다. 분리된 화합물들은 1차원 NMR과 2차원 NMR 및 mass 분석을 통하여 화학구조를 결정하였다. 분리된 화합물들의 생물활성은 항산화 활성과 neuraminidase 억제활성을 측정하였다. 항산화능은 ABTS radical 소거활성, DPPH radical 소거활성과 환원능 시험을 실시하였다. 화합물 2 와 3 이 농도 의존적으로 항산화능과 neuraminidase 억제활성을 보였다.
요 약 상황버섯은 약용버섯으로 소나무 비늘버섯과에 속하는 다양한 질병의 치료를 위해 전통 의학으로 사용 되어져 왔습니다. 본 연구에서는 상황버섯 균주 배양액의 화학적 구성성분과 생물활성을 연구하였다. 상황버섯 균주를 PDB 배지를 이용하여 배양하였고, 이 배양액을 ethyl acetate로 추출하였다. Ethyl acetate 분획물을 감압농축 한 후, ODS column chromatography와 Sephadex LH-20 column chromatography를 차례로 분리 정제 실험을 실시하였다. 최종적으로 Caffeic acid (1), inotilone (2), 4-(3,4-dihydroxyphenyl) -3-buten-2-one (3), phellinane H (4), (2E,4E)-(+)-4′-Hydroxy-α-ionylideneacetic acid (5) and (2E,4E)-α-ionylideneacetic acid (6) 6종의 화합물이 분리되었다. 분리된 화합물들은 1차원 NMR과 2차원 NMR 및 mass 분석을 통하여 화학구조를 결정하였다. 분리된 화합물들의 생물활성은 항산화 활성과 neuraminidase 억제활성을 측정하였다. 항산화능은 ABTS radical 소거활성, DPPH radical 소거활성과 환원능 시험을 실시하였다. 화합물 2 와 3 이 농도 의존적으로 항산화능과 neuraminidase 억제활성을 보였다.
Abstract The medicinal fungus Phellinus linteus in the family Hymenochaetaceae has been used as traditional medicine for the treatment of various diseases. In this study, we have investigated the chemical constituents of the cultured broth of P. linteus and their biological properties. P. linteus wa...
Abstract The medicinal fungus Phellinus linteus in the family Hymenochaetaceae has been used as traditional medicine for the treatment of various diseases. In this study, we have investigated the chemical constituents of the cultured broth of P. linteus and their biological properties. P. linteus was cultured in the potato dextrose broth medium, and the culture broth was extracted with ethyl acetate from the culture broth. The ethyl acetate-soluble portion was concentrated under reduced pressure and the concentrate was subjected to ODS column chromatography eluted with an increasing amount of methanol in water, followed by Sephadex LH-20 column chromatography eluted with aqueous methanol. Finally six compounds 1-6 were purified. The structures of these compounds were determined as caffeic acid (1), inotilone (2), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (3), phellilane H (4), (2E,4E)-(+)-4′-hydroxy-α-ionylideneacetic acid (5) and (2E,4E)-α-ionylideneacetic acid (6) by spectroscopic methods including one and two dimensional NMR and mass. The antioxidant and neuraminidase inhibitory activities of the isolated compounds were investigated. Compounds 2 and 3 exhibited in a dose-dependent manner antioxidant and neuraminidase inhibition activity.
Abstract The medicinal fungus Phellinus linteus in the family Hymenochaetaceae has been used as traditional medicine for the treatment of various diseases. In this study, we have investigated the chemical constituents of the cultured broth of P. linteus and their biological properties. P. linteus was cultured in the potato dextrose broth medium, and the culture broth was extracted with ethyl acetate from the culture broth. The ethyl acetate-soluble portion was concentrated under reduced pressure and the concentrate was subjected to ODS column chromatography eluted with an increasing amount of methanol in water, followed by Sephadex LH-20 column chromatography eluted with aqueous methanol. Finally six compounds 1-6 were purified. The structures of these compounds were determined as caffeic acid (1), inotilone (2), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (3), phellilane H (4), (2E,4E)-(+)-4′-hydroxy-α-ionylideneacetic acid (5) and (2E,4E)-α-ionylideneacetic acid (6) by spectroscopic methods including one and two dimensional NMR and mass. The antioxidant and neuraminidase inhibitory activities of the isolated compounds were investigated. Compounds 2 and 3 exhibited in a dose-dependent manner antioxidant and neuraminidase inhibition activity.
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