Akebia quinata DECAISENE (Lardizabalaceae) is a creeping woody vine that is widely distributed in East Asia, including Korea, China, and Japan. Traditionally, its dried stem is used mainly as a diuretic agent for the treatment of edema and rheumatic pain. Previous phytochemical investigations result...
Akebia quinata DECAISENE (Lardizabalaceae) is a creeping woody vine that is widely distributed in East Asia, including Korea, China, and Japan. Traditionally, its dried stem is used mainly as a diuretic agent for the treatment of edema and rheumatic pain. Previous phytochemical investigations resulted in the isolation of triterpenes, triterpene glycosides, phenylethanoid glycosides and megastigmane glycoside. Amyloid β (Aβ), a main component of the senile plaque detected in Alzheimer’s disease, induces cell death. However, a limited number of studies have addressed the biological and pharmacological effects of EtOAc fraction of Akebiae Caulis. In particular, the inhibitory activity against Aβ-42 induced fibrilogenesis of Akebiae Caulis remains unclear. In the present study, by means of repeated column chromatography using silicagel, LiChroprep RP-18, MCI-gel, and MPLC, nine compounds: Pulsatillasaponin A (1), Collinsonidin (2), Akebonic acid (3), Hederagenin (4), 1-(3′, 4′-dihydroxycinnamoyl) cyclopentane-2, 3-diol (5), Asperosaponin C (6), Leontoside A (7), Quinatic acid (8), and Quinatoside A (9) was isolated from the Akebiae Caulis.The chemical structures of compounds (1-9) were identified based on 1D and 2D NMR, including H-H COSY, HSQC, and HMBC spectroscopic analyses. Compound 5 and 6 were isolated from this plant for the first time. For these isolated compounds, the inhibitory activities of Aβ-42 induced fibrilogenesis were examined. Among these isolated compounds, compound 2,3,and 6 have significant inhibitory effects against Aβ-42 induced fibrilogenesis. This is the first report on the Aβ-42 inhibitory effects of the components from Akebiae Caulis.
Akebia quinata DECAISENE (Lardizabalaceae) is a creeping woody vine that is widely distributed in East Asia, including Korea, China, and Japan. Traditionally, its dried stem is used mainly as a diuretic agent for the treatment of edema and rheumatic pain. Previous phytochemical investigations resulted in the isolation of triterpenes, triterpene glycosides, phenylethanoid glycosides and megastigmane glycoside. Amyloid β (Aβ), a main component of the senile plaque detected in Alzheimer’s disease, induces cell death. However, a limited number of studies have addressed the biological and pharmacological effects of EtOAc fraction of Akebiae Caulis. In particular, the inhibitory activity against Aβ-42 induced fibrilogenesis of Akebiae Caulis remains unclear. In the present study, by means of repeated column chromatography using silicagel, LiChroprep RP-18, MCI-gel, and MPLC, nine compounds: Pulsatillasaponin A (1), Collinsonidin (2), Akebonic acid (3), Hederagenin (4), 1-(3′, 4′-dihydroxycinnamoyl) cyclopentane-2, 3-diol (5), Asperosaponin C (6), Leontoside A (7), Quinatic acid (8), and Quinatoside A (9) was isolated from the Akebiae Caulis.The chemical structures of compounds (1-9) were identified based on 1D and 2D NMR, including H-H COSY, HSQC, and HMBC spectroscopic analyses. Compound 5 and 6 were isolated from this plant for the first time. For these isolated compounds, the inhibitory activities of Aβ-42 induced fibrilogenesis were examined. Among these isolated compounds, compound 2,3,and 6 have significant inhibitory effects against Aβ-42 induced fibrilogenesis. This is the first report on the Aβ-42 inhibitory effects of the components from Akebiae Caulis.
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