본 연구에서는 Fruticiola sieboldiana종 달팽이 육질을 노루궁뎅이버섯균사체(Hericium erinaceum Mycelium, HE)로 1차 발효하고 이 발효물을 유산균(Leuconostoc mesenteroides)으로 2차 발효하여. 발효 단계별로 추출물의 아미노산 변화를 보았으며 이를 피부 개선에 활용하기 위하여 ...
본 연구에서는 Fruticiola sieboldiana종 달팽이 육질을 노루궁뎅이버섯균사체(Hericium erinaceum Mycelium, HE)로 1차 발효하고 이 발효물을 유산균(Leuconostoc mesenteroides)으로 2차 발효하여. 발효 단계별로 추출물의 아미노산 변화를 보았으며 이를 피부 개선에 활용하기 위하여 항노화 활성을 평가하였다. 발효 전 달팽이 추출물의 아미노산은 19종이었으며 총 아미노산 함량은 15.8 wt%이었다. 2차 발효 공정을 거친 달팽이 추출물의 아미노산은 76.7 wt%, 지방산류는 3.7 wt%, 다당체는 16.7 wt%, 수분 2.9 wt% 및 기타성분 4.18 wt%이었다. 발효를 통하여 아미노산의 19종은 변함없이 일치하였으며 총 아미노산 함량은 1·2차 발효를 통하여 발효 전 대비 각각 37.4 wt%, 76.7 wt%로 136%, 285% 증가하였다. 특히 2차 발효 추출물에서는 serine, glycine, arginine, proline의 함량이 4배 가량 증가함을 알 수 있었다. 2차 발효 추출물의 1,1-diphenyl-2-picryl hydrazyl(DPPH) free radical 소거 활성 효과(IC50%)는 7.3 μg/mL로 발효 전 달팽이 추출물보다 약 2배 높은 효과를 나타내었고, 엘라스테이즈(elastase)의 활성 저해력(IC50%)은 녹차 추출물보다 29.2% 높았으며, 발효 전 달팽이 추출물 보다는 41.2% 우수하였다. 2차 발효 달팽이 추출물의 티로시나아제의 발현 억제능(IC50%)은 녹차 추출물보다 44.0% 높았고, 발효 전 달팽이 추출물 보다는 177.6% 우수하였다. 2차 발효 달팽이 추출물의 피부 섬유아세포의 성장률은 녹차 추출물보다 27%의 높았으며, 발효 전 달팽이 추출물 보다는 16% 우수하였다. 2차 발효 달팽이 추출물의 콜라겐 생합성율은 녹차 추출물보다 31%의 높았고, 발효 전 달팽이추출물 보다는 24.8% 우수하였다. 2차 발효 달팽이 추출물의 피부 개선 효능을 확인하고자 추출물을 5% 함유한 시트 마스크 팩을 제조하여 주 3회 4주간 30대 여성을 대상으로 사용한 후 보습량, 탄력, 거칠기 및 멜라닌 색소 변화를 측정하였다. 이 결과 2단계 발효 달팽이 추출물을 5%함유한 마스크 팩 (two-step fermentation snail extraction mask-pack : 2F-SEM)을 사용한 경우 추출물을 넣지 않은 플라세보 마스크 팩 (placebo mask-pack: PM)보다 평균적으로 보습량은 11.0 % 증가하였고 주름 개선효과는 5.1% 개선되었다. 색소 침착은 15.0% 감소하여 멜라닌 감소 효과를 보였다. 이를 통하여 2단계 발효 달팽이 추출물은 항노화 화장품 원료로서의 활용 가능성이 있을 것으로 판단된다. 마스크 팩에 대한 관능 평가는 부드러움(softness), 보습감(moisture) 및 주름 개선효과(fine wrinkle improvement)에서 유의한 차이를 보였다.
본 연구에서는 Fruticiola sieboldiana종 달팽이 육질을 노루궁뎅이버섯균사체(Hericium erinaceum Mycelium, HE)로 1차 발효하고 이 발효물을 유산균(Leuconostoc mesenteroides)으로 2차 발효하여. 발효 단계별로 추출물의 아미노산 변화를 보았으며 이를 피부 개선에 활용하기 위하여 항노화 활성을 평가하였다. 발효 전 달팽이 추출물의 아미노산은 19종이었으며 총 아미노산 함량은 15.8 wt%이었다. 2차 발효 공정을 거친 달팽이 추출물의 아미노산은 76.7 wt%, 지방산류는 3.7 wt%, 다당체는 16.7 wt%, 수분 2.9 wt% 및 기타성분 4.18 wt%이었다. 발효를 통하여 아미노산의 19종은 변함없이 일치하였으며 총 아미노산 함량은 1·2차 발효를 통하여 발효 전 대비 각각 37.4 wt%, 76.7 wt%로 136%, 285% 증가하였다. 특히 2차 발효 추출물에서는 serine, glycine, arginine, proline의 함량이 4배 가량 증가함을 알 수 있었다. 2차 발효 추출물의 1,1-diphenyl-2-picryl hydrazyl(DPPH) free radical 소거 활성 효과(IC50%)는 7.3 μg/mL로 발효 전 달팽이 추출물보다 약 2배 높은 효과를 나타내었고, 엘라스테이즈(elastase)의 활성 저해력(IC50%)은 녹차 추출물보다 29.2% 높았으며, 발효 전 달팽이 추출물 보다는 41.2% 우수하였다. 2차 발효 달팽이 추출물의 티로시나아제의 발현 억제능(IC50%)은 녹차 추출물보다 44.0% 높았고, 발효 전 달팽이 추출물 보다는 177.6% 우수하였다. 2차 발효 달팽이 추출물의 피부 섬유아세포의 성장률은 녹차 추출물보다 27%의 높았으며, 발효 전 달팽이 추출물 보다는 16% 우수하였다. 2차 발효 달팽이 추출물의 콜라겐 생합성율은 녹차 추출물보다 31%의 높았고, 발효 전 달팽이추출물 보다는 24.8% 우수하였다. 2차 발효 달팽이 추출물의 피부 개선 효능을 확인하고자 추출물을 5% 함유한 시트 마스크 팩을 제조하여 주 3회 4주간 30대 여성을 대상으로 사용한 후 보습량, 탄력, 거칠기 및 멜라닌 색소 변화를 측정하였다. 이 결과 2단계 발효 달팽이 추출물을 5%함유한 마스크 팩 (two-step fermentation snail extraction mask-pack : 2F-SEM)을 사용한 경우 추출물을 넣지 않은 플라세보 마스크 팩 (placebo mask-pack: PM)보다 평균적으로 보습량은 11.0 % 증가하였고 주름 개선효과는 5.1% 개선되었다. 색소 침착은 15.0% 감소하여 멜라닌 감소 효과를 보였다. 이를 통하여 2단계 발효 달팽이 추출물은 항노화 화장품 원료로서의 활용 가능성이 있을 것으로 판단된다. 마스크 팩에 대한 관능 평가는 부드러움(softness), 보습감(moisture) 및 주름 개선효과(fine wrinkle improvement)에서 유의한 차이를 보였다.
This study was conducted to investigate the effects of two fermentation methods using Hericium erinaceum Mycelium (HE) and second fermentation using Leuconostoc mesenteroides by using snail meat of Fruticiola sieboldiana species, The anti-aging activity of the snail extract was evaluated. Amino...
This study was conducted to investigate the effects of two fermentation methods using Hericium erinaceum Mycelium (HE) and second fermentation using Leuconostoc mesenteroides by using snail meat of Fruticiola sieboldiana species, The anti-aging activity of the snail extract was evaluated. Amino acid content of the snail extract before fermentation was 19 species. The total amino acid content was 15.8wt%. The total amino acid content of the snail extract analyzed by the second fermentation process was 76.7 wt%. Fatty acids were 3.7 wt%. The polysaccharide content was 16.7wt%. The moisture content was 2.9 wt%. Other ingredients were 4.2 wt%. Through the fermentation, 19 kinds of amino acids were unchanged. The total amino acid content increased by 136% and 285% to 37.4 wt% and 76.7 wt%, respectively, after the 1st and 2nd fermentation.In particular, the contents of four amino acids of serine, glycine, arginine, and proline were significantly increased. The DPPH free Radical scavenging activity (IC50%) of the extract was 7.3 mg/mL, which was twice as good as that of the snail extract before fermentation. The inhibitory effect of elastin inhibitor (IC50%) on the secondary fermented snail extract was 29.2% higher than that of the control group. And it was more than 41.2% more than the ordinary extract. The inhibitory activity of tyrosinase (IC50%) of the 2nd fermented snail extract was 44.0% higher than that of the control group. It was more than 177.6% more than the ordinary extract. The growth rate of fibroblast of the second fermented snail extract was 27% higher than that of the control group. And it was more than 16% higher than the ordinary extract. The collagen biosynthesis rate of the second fermented snail extract was 31% higher than that of the control group. And it was more than 24.8% higher than the general extract. As a clinical study, a sheet mask pack containing 5% of a snail fermented extract was prepared to confirm skin improvement effect of the second fermented snail extract. The application method was applied to the skin three times a week for 4 weeks for woman in her thirties and the moisturizing effect, the elasticity improvement, the roughness, the melanin pigment and the wrinkle improvement were studied. As a result, the melanin-reducing effect of the 2F-SEM was decreased by 15.0% or more than that of PM. Wrinkle improvement was improved by 5.1%. Pigmentation was decreased by 15.0% and melanin reduction effect was shown. Sensory evaluation of mask pack showed excellent difference in softness, moisture feel, and fine wrinkle improvement. Secondary fermented snail extracts obtained through this study are expected to be used as raw materials for cosmetics with antioxidant effect.
This study was conducted to investigate the effects of two fermentation methods using Hericium erinaceum Mycelium (HE) and second fermentation using Leuconostoc mesenteroides by using snail meat of Fruticiola sieboldiana species, The anti-aging activity of the snail extract was evaluated. Amino acid content of the snail extract before fermentation was 19 species. The total amino acid content was 15.8wt%. The total amino acid content of the snail extract analyzed by the second fermentation process was 76.7 wt%. Fatty acids were 3.7 wt%. The polysaccharide content was 16.7wt%. The moisture content was 2.9 wt%. Other ingredients were 4.2 wt%. Through the fermentation, 19 kinds of amino acids were unchanged. The total amino acid content increased by 136% and 285% to 37.4 wt% and 76.7 wt%, respectively, after the 1st and 2nd fermentation.In particular, the contents of four amino acids of serine, glycine, arginine, and proline were significantly increased. The DPPH free Radical scavenging activity (IC50%) of the extract was 7.3 mg/mL, which was twice as good as that of the snail extract before fermentation. The inhibitory effect of elastin inhibitor (IC50%) on the secondary fermented snail extract was 29.2% higher than that of the control group. And it was more than 41.2% more than the ordinary extract. The inhibitory activity of tyrosinase (IC50%) of the 2nd fermented snail extract was 44.0% higher than that of the control group. It was more than 177.6% more than the ordinary extract. The growth rate of fibroblast of the second fermented snail extract was 27% higher than that of the control group. And it was more than 16% higher than the ordinary extract. The collagen biosynthesis rate of the second fermented snail extract was 31% higher than that of the control group. And it was more than 24.8% higher than the general extract. As a clinical study, a sheet mask pack containing 5% of a snail fermented extract was prepared to confirm skin improvement effect of the second fermented snail extract. The application method was applied to the skin three times a week for 4 weeks for woman in her thirties and the moisturizing effect, the elasticity improvement, the roughness, the melanin pigment and the wrinkle improvement were studied. As a result, the melanin-reducing effect of the 2F-SEM was decreased by 15.0% or more than that of PM. Wrinkle improvement was improved by 5.1%. Pigmentation was decreased by 15.0% and melanin reduction effect was shown. Sensory evaluation of mask pack showed excellent difference in softness, moisture feel, and fine wrinkle improvement. Secondary fermented snail extracts obtained through this study are expected to be used as raw materials for cosmetics with antioxidant effect.
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