Vitamins are essential micronutrients for the normal functioning of the human body. Since vitamins are not synthesized in the human body, they must be consumed daily as food or supplements. Among vitamins, in particular, water-soluble vitamin B7 (biotin) and B12(cobalmins) are present at a trace lev...
Vitamins are essential micronutrients for the normal functioning of the human body. Since vitamins are not synthesized in the human body, they must be consumed daily as food or supplements. Among vitamins, in particular, water-soluble vitamin B7 (biotin) and B12(cobalmins) are present at a trace level. In this study, simultaneous extraction, immunoaffinity clean-up and HPLC was applied for determination of vitamin B7 and B12 in seafood and validated.
Sodium phosphate buffer (0.15 M, pH 7.0) and sodium acetate trihydrate buffer (0.2 M, pH 4.0) were tested for simultaneous extraction of vitamin B7 and B12, Different extraction methods including enzymatic extraction, ultra-sonication (none, 10 min at room temp., 30 min at 50℃) and heating (30 min at 100℃ and 121℃) were tested for simultaneous extraction of vitamin B7 and B12,
The biotin and cobalamin contents of the standard reference material (SRM 3280-multi vitamin) were simultaneously analyzed under the different extraction conditions (ultra-sonication treatment, temperature and pH). Recovery of biotin (96.2%) and
cyanocobalamin (83.9%) were within the acceptable range in heat treated (100°C) extraction followed by ultra-sonication (10 min at room temperature) using 0.2 M sodium acetate trihydrate buffer(pH 4.0) extraction solution. Recovery of biotin (96.9%) and cyanocobalamin from SRM 1849a by simultaneous analysis were (97.0%) were within the acceptable range. The coefficient variations (CV) of repeatability and reproducibility of biotin analysis were 4.7% and 4.0%, respectively. The CVs for repeatability and reproducibility of cyanocobalamin analysis were 4.8% and 5.2%, respectively. Extraction efficiencies of individual and simultaneous analyses for biotin and cobalamins were compared from 5 kinds of seafood (Japanese-Spanish mackerel, squid, ark shell, Japanese squillid mantis shrimp, and common sea squirt). Percent recovery of biotin and cyanocobalamin by simultaneous analysis were 71.4~129.0% and 63.6~103.0%, respectively. In this study, a simple and rapid simultaneous extraction and immunoaffinity-HPLC analysis for biotin and cobalamins was developed and validated, which was efficient for the determination of biotin and cobalamins in seafood.
Vitamins are essential micronutrients for the normal functioning of the human body. Since vitamins are not synthesized in the human body, they must be consumed daily as food or supplements. Among vitamins, in particular, water-soluble vitamin B7 (biotin) and B12(cobalmins) are present at a trace level. In this study, simultaneous extraction, immunoaffinity clean-up and HPLC was applied for determination of vitamin B7 and B12 in seafood and validated.
Sodium phosphate buffer (0.15 M, pH 7.0) and sodium acetate trihydrate buffer (0.2 M, pH 4.0) were tested for simultaneous extraction of vitamin B7 and B12, Different extraction methods including enzymatic extraction, ultra-sonication (none, 10 min at room temp., 30 min at 50℃) and heating (30 min at 100℃ and 121℃) were tested for simultaneous extraction of vitamin B7 and B12,
The biotin and cobalamin contents of the standard reference material (SRM 3280-multi vitamin) were simultaneously analyzed under the different extraction conditions (ultra-sonication treatment, temperature and pH). Recovery of biotin (96.2%) and
cyanocobalamin (83.9%) were within the acceptable range in heat treated (100°C) extraction followed by ultra-sonication (10 min at room temperature) using 0.2 M sodium acetate trihydrate buffer(pH 4.0) extraction solution. Recovery of biotin (96.9%) and cyanocobalamin from SRM 1849a by simultaneous analysis were (97.0%) were within the acceptable range. The coefficient variations (CV) of repeatability and reproducibility of biotin analysis were 4.7% and 4.0%, respectively. The CVs for repeatability and reproducibility of cyanocobalamin analysis were 4.8% and 5.2%, respectively. Extraction efficiencies of individual and simultaneous analyses for biotin and cobalamins were compared from 5 kinds of seafood (Japanese-Spanish mackerel, squid, ark shell, Japanese squillid mantis shrimp, and common sea squirt). Percent recovery of biotin and cyanocobalamin by simultaneous analysis were 71.4~129.0% and 63.6~103.0%, respectively. In this study, a simple and rapid simultaneous extraction and immunoaffinity-HPLC analysis for biotin and cobalamins was developed and validated, which was efficient for the determination of biotin and cobalamins in seafood.
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