본 연구는 천연추출물을 이용하여 건강식품과 기능성 천연화장품으 로, 또는 수인성 전염병을 예방 할 수 있는 의학용으로 이용할 수 있 는 베리류 추출물 6종의 항산화 분석과 항균활성 효과에 대하여 실 험을 실시하였다. 베리류 과수 가운데 블루베리, ...
본 연구는 천연추출물을 이용하여 건강식품과 기능성 천연화장품으 로, 또는 수인성 전염병을 예방 할 수 있는 의학용으로 이용할 수 있 는 베리류 추출물 6종의 항산화 분석과 항균활성 효과에 대하여 실 험을 실시하였다. 베리류 과수 가운데 블루베리, 아로니아, 복분자, 오디, 산딸기, 라즈 베리 등 6종의 추출물을 이용하여 총폴리페놀 함량을 측정하고, DPPH법을 이용하여 항산화 활성을 측정하여 상호간의 상관 관계를 분석하였다. 그 결과 총 폴리페놀 함량은 아로니아> 복분자> 오디> 블루베리> 라 즈베리> 산딸기 순으로 나타났으며, 항산화 활성은 아로니아> 라즈베 리 >복분자> 블루베리> 오디> 산딸기 순으로 나타났다. 이러한 결과 로 총폴리페놀 함량과 항산화 활성과의 상관관계가 있다는 것을 확인 하였으며, 그 중에서도 아로니아와 복분자가 강력한 항산화 활성이 있다는 것을 확인하였다. 그러나 라즈베리의 경우는 총폴리페놀 함량이 낮은데도 불구하고 항 산화 활성이 높았다. 따라서 앞으로 라즈베리에 대한 선행연구가 필 요하리라 생각된다. 또한, 피부 노화나 피부 질환과 관련 있는 6종의 균주인 Cutibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Pseudo -monas aeruginosa, Escherichia coli, Candida albicans 를 사용하여 항균 효과에 대하여 비교 실험을 한 결과, 아로니아는 여드름균( C. acnes ), 라즈베리는 황색포도상구균 ( S. aureus )과 표피포도상구균( S. epidermidis ), 복분자는 대장 균( E. coli )처리구에서 가장 양호한 항균 활성을 나타내었다. 블루 베리, 오디, 산딸기 등은 대체적으로 항산화성과 항균활성이 양호하 게 나타났다. 녹농균(P.aeruginosa ) 처리구에서는 다른 베리류의 항균 활성 효과 에 비하여 상대적으로 항균활성이 낮았으며, 칸디다균(C.albicans )의 항균활성은 오디처리구 에서만 항균 활성이 나타났으며 다른 베리류 에는 항균 효과가 나타나지 않았다. 이와 같은 결과들로 미루어 볼 때 6종 베리류( 블루베리, 아로니아, 복분자, 오디, 산딸기, 라즈베리 ) 추출물은 농도 의존적으로 항산화 와 항균 활성에 대한 효과가 양호하였으며, 이러한 연구결과들을 활 용하여 건강 보조식품이나 항균이나 트러블, 염증 억제제로 이용가치 가 있어 기능성 화장품의 신소재 개발로도 가능하다고 판단된다.
본 연구는 천연추출물을 이용하여 건강식품과 기능성 천연화장품으 로, 또는 수인성 전염병을 예방 할 수 있는 의학용으로 이용할 수 있 는 베리류 추출물 6종의 항산화 분석과 항균활성 효과에 대하여 실 험을 실시하였다. 베리류 과수 가운데 블루베리, 아로니아, 복분자, 오디, 산딸기, 라즈 베리 등 6종의 추출물을 이용하여 총폴리페놀 함량을 측정하고, DPPH법을 이용하여 항산화 활성을 측정하여 상호간의 상관 관계를 분석하였다. 그 결과 총 폴리페놀 함량은 아로니아> 복분자> 오디> 블루베리> 라 즈베리> 산딸기 순으로 나타났으며, 항산화 활성은 아로니아> 라즈베 리 >복분자> 블루베리> 오디> 산딸기 순으로 나타났다. 이러한 결과 로 총폴리페놀 함량과 항산화 활성과의 상관관계가 있다는 것을 확인 하였으며, 그 중에서도 아로니아와 복분자가 강력한 항산화 활성이 있다는 것을 확인하였다. 그러나 라즈베리의 경우는 총폴리페놀 함량이 낮은데도 불구하고 항 산화 활성이 높았다. 따라서 앞으로 라즈베리에 대한 선행연구가 필 요하리라 생각된다. 또한, 피부 노화나 피부 질환과 관련 있는 6종의 균주인 Cutibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Pseudo -monas aeruginosa, Escherichia coli, Candida albicans 를 사용하여 항균 효과에 대하여 비교 실험을 한 결과, 아로니아는 여드름균( C. acnes ), 라즈베리는 황색포도상구균 ( S. aureus )과 표피포도상구균( S. epidermidis ), 복분자는 대장 균( E. coli )처리구에서 가장 양호한 항균 활성을 나타내었다. 블루 베리, 오디, 산딸기 등은 대체적으로 항산화성과 항균활성이 양호하 게 나타났다. 녹농균(P.aeruginosa ) 처리구에서는 다른 베리류의 항균 활성 효과 에 비하여 상대적으로 항균활성이 낮았으며, 칸디다균(C.albicans )의 항균활성은 오디처리구 에서만 항균 활성이 나타났으며 다른 베리류 에는 항균 효과가 나타나지 않았다. 이와 같은 결과들로 미루어 볼 때 6종 베리류( 블루베리, 아로니아, 복분자, 오디, 산딸기, 라즈베리 ) 추출물은 농도 의존적으로 항산화 와 항균 활성에 대한 효과가 양호하였으며, 이러한 연구결과들을 활 용하여 건강 보조식품이나 항균이나 트러블, 염증 억제제로 이용가치 가 있어 기능성 화장품의 신소재 개발로도 가능하다고 판단된다.
This study is about the physiological activity analysis and antibacterial effect of berries produced in Korea, such as Vaccinium corymbosum (Blueberry), Aronia melanocarpa (Aronia), Rubus coreanus (Korean Blueberry),Morus alba (Mulberry), Rubus crataegifolius (Laspberry), Rubus idaeu...
This study is about the physiological activity analysis and antibacterial effect of berries produced in Korea, such as Vaccinium corymbosum (Blueberry), Aronia melanocarpa (Aronia), Rubus coreanus (Korean Blueberry),Morus alba (Mulberry), Rubus crataegifolius (Laspberry), Rubus idaeus (Western Laspberry). An extract was prepared using the 6 types of berries as a material, and antioxidant and antibacterial effects were measured and compared. The total polyphenol content was superior and high with Aronia melanocarpa (Am) at 41.31±1.69 TAE (Tannic acid quivalent,mg/g), and the Rubus coreanus (Rc) at 30.78±0.56 TAE mg/g, Morus alba (Ma) at 23.9±1.69 TAE (TAE) Vaccinium corymbosum (Vc) at 23.04±0.9 TAE mg/g,Rubus idaeus (Ri) 19.99±0.81 TAE mg/g, Rubus crataegifolius (Rcr) was high. The order of the total polyphenol content is Am > Rc > Ma > Vc, Ri > and Rcr. Among the six kinds of berries, Aronia has various physiological activities such as polymeric roanthocyanidins, chlorogenic acid, neochlorogenic acid, cyanidin-3-Ogalactoxide, and cyanidin-3-O-arabinoxide, and is reported to have a higher amount of berries than other foods and functional materials. The antioxidant activity was confirmed as DPPH radical scavenging activity, and the results showed that Aronia melanocarpa had a significantly higher electron donation capacity of 134.49±2.71%, which seems to have excellent antioxidant activity due to its abundance of anthocyanin, polyphenol, and flavonoids. Next, Rubus idaeus was 125.46±2.14%, Rubus oreanus was 118.18±3.09%, and Vaccinium corymbosum was more than 100%, showing that the overall DPPH erasing activity was high, showing that the antioxidant activity of berries was strong. However, Morus alba and Rubus crataegifolius were 69.49±1.91% and 25.06±1.16%, which had relatively low antioxidant activity compared to other berries. It can be seen that as the total polyphenol content increases, the antioxidant activity is actively proportional. EDA (%): Am >Ri > Rc > Vc > Ma > and Rcr. To measure the antibacterial properties of extracts for 6 types of berries, Cutibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans , which are mainly related to skin aging or skin diseases, And Citrus grandis Osbeck was used as a control group, and antimicrobial inhibition was measured using paper disc diffusion method to check the surrounding area. As a result, Cutibacterium acnes , which are acne bacteria, showed high antibacterial activity with Aronia melanocarpa (Am), Morus alba (Ma), and Rubus crataegifolius (Rcr) showing 20mm clear zone at 40㎕/disc, and Vaccinium corymbosum (Vc) and Rubus idaeus (Ri) did not show a clear zone at 10μ l, so the concentration of 16mm was not increased. It can be seen that the antibacterial effect increases as the concentration increases for each sample. The order of antibacterial activity is Am > Ma, Rcr > Vc > Rc, and Ri. Staphylococcus aureus is a factor bacteria found in 90% of atopic dermatitis, and is a representative causative agent that invades pore infections and wounds and causes anger, and is more pronounced in Rubus idaeus (Ri) and Rubus crataegifolius (Rc). In 40 μl of sample, the clean zone showed a high antibacterial effect with Rubus idaeus (Ri) of 19 mm and Rubus crataegifolius (Rc)of 18 mm. At 30 μl, Aronia melanocarpa (Am) and Rubus crataegifolius (Rcr) showed antibacterial effects. In Morus alba (Ma) and Vaccinium corymbosum (Vc) the clear zone reaction was weaker than that of the control group in samples 10 and 20 μl, but the antibacterial effect could be measured. The antimicrobial activity order is Ri >Rc >Rcr >Am >Vc > and Ma. Tapylococcus epidermidis exists as a bacterium in the skin or mucous membrane, and raspberry Rubus idaeus (Ri) showed the strongest antibacterial activity at 25mm in sample 40μl, and Rubus coreanus (Rc)did not show a clear zone at 10μl, but when it was 40μl, it did not show antibacterial activity at 18mm when it was 10mm~30mm. The antimicrobial activity order is Ri > Am, Rc, Rcr > Vc > and Ma Pseudomonas aeruginosa is a pathogenic bacterium that causes opportunistic infections, such as sepsis, which is fatal to the human body, or infection in hospitals. As a result of observing the clean zone formation, Rubus idaeus (Ri) showed the highest antibacterial activity from sample 40 μl to clear zone of 16mm, but Aronia melanocarpa (Am) and Rubus coreanus (Rc) showed no reaction from 10 μl to Vaccinium corymbosum (Vc)20 μl , especially Morus alba (Ma) showed no reaction from 40 μl to 9mm clear zone. It was Morus alba (Ma) firmed that 6 types of berries had a weaker antibacterial effect compared to other strains such as control groups in P.aeruginosa. The antimicrobial activity order is Ri > Am > Rcr, Rc > Vc > and Ma. Escherichia coli is mostly non-pathogenic, but some are important causative bacteria of gastrointestinal diseases, accounting for about 80% of enteritis and urinary tract infections. As a result of observing clean zone formation for E.coli , clean zone is shown as a whole, so antibacterial activity is excellent. The Rubus coreanus (Rc) showed the highest antibacterial activity from 10 to 40 μl to 20 to 28 mm. Rubus crataegifolius (Rcr) had high antibacterial activity at 30 μl, and there was no clear zone change even at 40 μl with high concentration. Aronia melanocarpa (Am) did not appear at 10 μl, but it is believed that antibacterial activity begins with a clear zone of 10 mm from 20 μl. Blueberries, Rubus crataegifolius (Rcr), and raspberries (Vc, and Ri) had slightly higher antibacterial activity compared to the control group. Five types of berries, excluding Aronia melanocarpa (Am), showed high antibacterial activity with a clear zone of 25 to 28 mm in E. coli. a ticket Antimicrobial activity is in the order of Rc>Rcr>Vc, Ri, Ma>and Am. Candida albicans exist in groups of normal bacteria in various parts of the human body, including the human respiratory system, digestive system, skin, and mucous membrane, and can cause opportunity infection if the immune system weakens. As a result of observing the formation of clean zones for C. albicans, odiman clean zones were seen and were not found in other berries. C. albicans showed high antibacterial activity at 40㎕ to 26 mm only in Morus alba (Ma). Clear zones were not shown in the other 5 berries, and there was no weak or antibacterial activity against C. albicans.
This study is about the physiological activity analysis and antibacterial effect of berries produced in Korea, such as Vaccinium corymbosum (Blueberry), Aronia melanocarpa (Aronia), Rubus coreanus (Korean Blueberry),Morus alba (Mulberry), Rubus crataegifolius (Laspberry), Rubus idaeus (Western Laspberry). An extract was prepared using the 6 types of berries as a material, and antioxidant and antibacterial effects were measured and compared. The total polyphenol content was superior and high with Aronia melanocarpa (Am) at 41.31±1.69 TAE (Tannic acid quivalent,mg/g), and the Rubus coreanus (Rc) at 30.78±0.56 TAE mg/g, Morus alba (Ma) at 23.9±1.69 TAE (TAE) Vaccinium corymbosum (Vc) at 23.04±0.9 TAE mg/g,Rubus idaeus (Ri) 19.99±0.81 TAE mg/g, Rubus crataegifolius (Rcr) was high. The order of the total polyphenol content is Am > Rc > Ma > Vc, Ri > and Rcr. Among the six kinds of berries, Aronia has various physiological activities such as polymeric roanthocyanidins, chlorogenic acid, neochlorogenic acid, cyanidin-3-Ogalactoxide, and cyanidin-3-O-arabinoxide, and is reported to have a higher amount of berries than other foods and functional materials. The antioxidant activity was confirmed as DPPH radical scavenging activity, and the results showed that Aronia melanocarpa had a significantly higher electron donation capacity of 134.49±2.71%, which seems to have excellent antioxidant activity due to its abundance of anthocyanin, polyphenol, and flavonoids. Next, Rubus idaeus was 125.46±2.14%, Rubus oreanus was 118.18±3.09%, and Vaccinium corymbosum was more than 100%, showing that the overall DPPH erasing activity was high, showing that the antioxidant activity of berries was strong. However, Morus alba and Rubus crataegifolius were 69.49±1.91% and 25.06±1.16%, which had relatively low antioxidant activity compared to other berries. It can be seen that as the total polyphenol content increases, the antioxidant activity is actively proportional. EDA (%): Am >Ri > Rc > Vc > Ma > and Rcr. To measure the antibacterial properties of extracts for 6 types of berries, Cutibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans , which are mainly related to skin aging or skin diseases, And Citrus grandis Osbeck was used as a control group, and antimicrobial inhibition was measured using paper disc diffusion method to check the surrounding area. As a result, Cutibacterium acnes , which are acne bacteria, showed high antibacterial activity with Aronia melanocarpa (Am), Morus alba (Ma), and Rubus crataegifolius (Rcr) showing 20mm clear zone at 40㎕/disc, and Vaccinium corymbosum (Vc) and Rubus idaeus (Ri) did not show a clear zone at 10μ l, so the concentration of 16mm was not increased. It can be seen that the antibacterial effect increases as the concentration increases for each sample. The order of antibacterial activity is Am > Ma, Rcr > Vc > Rc, and Ri. Staphylococcus aureus is a factor bacteria found in 90% of atopic dermatitis, and is a representative causative agent that invades pore infections and wounds and causes anger, and is more pronounced in Rubus idaeus (Ri) and Rubus crataegifolius (Rc). In 40 μl of sample, the clean zone showed a high antibacterial effect with Rubus idaeus (Ri) of 19 mm and Rubus crataegifolius (Rc)of 18 mm. At 30 μl, Aronia melanocarpa (Am) and Rubus crataegifolius (Rcr) showed antibacterial effects. In Morus alba (Ma) and Vaccinium corymbosum (Vc) the clear zone reaction was weaker than that of the control group in samples 10 and 20 μl, but the antibacterial effect could be measured. The antimicrobial activity order is Ri >Rc >Rcr >Am >Vc > and Ma. Tapylococcus epidermidis exists as a bacterium in the skin or mucous membrane, and raspberry Rubus idaeus (Ri) showed the strongest antibacterial activity at 25mm in sample 40μl, and Rubus coreanus (Rc)did not show a clear zone at 10μl, but when it was 40μl, it did not show antibacterial activity at 18mm when it was 10mm~30mm. The antimicrobial activity order is Ri > Am, Rc, Rcr > Vc > and Ma Pseudomonas aeruginosa is a pathogenic bacterium that causes opportunistic infections, such as sepsis, which is fatal to the human body, or infection in hospitals. As a result of observing the clean zone formation, Rubus idaeus (Ri) showed the highest antibacterial activity from sample 40 μl to clear zone of 16mm, but Aronia melanocarpa (Am) and Rubus coreanus (Rc) showed no reaction from 10 μl to Vaccinium corymbosum (Vc)20 μl , especially Morus alba (Ma) showed no reaction from 40 μl to 9mm clear zone. It was Morus alba (Ma) firmed that 6 types of berries had a weaker antibacterial effect compared to other strains such as control groups in P.aeruginosa. The antimicrobial activity order is Ri > Am > Rcr, Rc > Vc > and Ma. Escherichia coli is mostly non-pathogenic, but some are important causative bacteria of gastrointestinal diseases, accounting for about 80% of enteritis and urinary tract infections. As a result of observing clean zone formation for E.coli , clean zone is shown as a whole, so antibacterial activity is excellent. The Rubus coreanus (Rc) showed the highest antibacterial activity from 10 to 40 μl to 20 to 28 mm. Rubus crataegifolius (Rcr) had high antibacterial activity at 30 μl, and there was no clear zone change even at 40 μl with high concentration. Aronia melanocarpa (Am) did not appear at 10 μl, but it is believed that antibacterial activity begins with a clear zone of 10 mm from 20 μl. Blueberries, Rubus crataegifolius (Rcr), and raspberries (Vc, and Ri) had slightly higher antibacterial activity compared to the control group. Five types of berries, excluding Aronia melanocarpa (Am), showed high antibacterial activity with a clear zone of 25 to 28 mm in E. coli. a ticket Antimicrobial activity is in the order of Rc>Rcr>Vc, Ri, Ma>and Am. Candida albicans exist in groups of normal bacteria in various parts of the human body, including the human respiratory system, digestive system, skin, and mucous membrane, and can cause opportunity infection if the immune system weakens. As a result of observing the formation of clean zones for C. albicans, odiman clean zones were seen and were not found in other berries. C. albicans showed high antibacterial activity at 40㎕ to 26 mm only in Morus alba (Ma). Clear zones were not shown in the other 5 berries, and there was no weak or antibacterial activity against C. albicans.
AI-Helper
※ AI-Helper는 부적절한 답변을 할 수 있습니다.