• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

무지개송어의 간세포 초대배양에 의한 Vitellogeinin 합성 유도

Vitellogenin Induction by Rainbow trout (Oncorhynchus mykiss) Hepatocytes in Primary Culture


무지개송어의 초대배양 간세포에 있어서 Vitellogenin (VTG)의 합성 유도는 전기영동적 수법에 의해 행하였다. 간세포는 7일 동안 phositively charged dish를 이용한 배양으로 단층 확산되었다. 배양 7일의 간세포 생존율은 $E_2$의 유무에 따라 각각 20.7% 및 23.6%가 감소하였으며, DNA함유량도 각각 13.7% 및 14.0%가 감소하였다. $E_2$ 첨가군과 대조군간에는 생존율과 DNA 함유량에 있어서는 유의한 차이가 나타나지 않았다. 그리고, 총 단백질에 대한 VTG의 비율은 $E_2$$10^{-6}$ M의 첨가시에 최대치를 나타내었다. 그러나, 고농도($10^{-5}$M)에서는 오히려 감소하는 경향을 나타내었다.


Vitellogenin (VTG) induction in response estradiol-17${\beta}$ ($E_2$) were electrophoretically examined in primary hepatocyte cultures in rainbow trout. The hepatocytes were maintained as monolayers on positively charged dishes for up to 7 days. The viability of hepatocytes on Day 7 in cultures decreased about 20.7% and 23.6% with and without $E_2$, respecitively. The amount of DNA per dish also decreased to 13.7% and 14.0% with and without $E_2$, respectively. There were no differences in viability and DNA content between the control and $E_2$-treated culture. Moreover, the rate of VTG to total protein concentrations reached the maxium level at the addition of $10^{-6}$ M E2, to the incubation medium. However, the higher concentration of $10^{-5}$ M $E_2$ rater depressed the level.

참고문헌 (21)

  1. Induction of vitellogenin in primary monolayer cultures of cockerel hepatocytes , Boehm, K. D.;R. L. Hood;J. Ilan , Proc. natl. Acad. Sci. U.S.A. / v.85,pp.3450-3454, 1988
  2. Two sites of glucose control of insulin release with distinct dependence on the energy state in pancreatic B-cells , Detimary, P.;P. Gilon;M. Nenquin;J.-C. Henquin , Biochem. J. / v.297,pp.455-461, 1994
  3. Comparative studies on immunochemical properties of female-specific serum protein and egg yolk proteins in rainbow trout (Salmo gairdneri) , Hara, A.;H. Hirai , Comp. Biochem. Physiol. / v.59B,pp.339-343, 1978
  4. Gonadotropin-releasing hormone-stimulated intracellular $Ca^{2+}$ fluctuations and luteinizing hormone release can be uncoupled from inositol phosphate production , Hawes, B. E.;S. B. Waters;J. A. Janovick;J. E. Bleasdale;P. M. Conn , Endocrinology / v.130,pp.3475-3483, 1992
  5. Use of trout serum to prepare primary attached monolayer cultures of hepatocytes from rainbow trout (Salmo gairdneri) , Kocal, T.;B. A. Quinn;I. R. Smith;H. W. Ferguson;M. A. Hayes , In Vitro Cell. Dev. Biol. / v.24,pp.304-308, 1988
  6. Vitellogenin induction by estradiol-17βin primary hepatocyte culture in the rainbow trout, Oncorhynchus mykiss , Kwon, H. C.;S. Hayashi;Y. Mugiya , Comp. Biochem. Physiol. / v.104B,pp.381-396, 1997
  7. Cleavage of structural proteins during assembly of the head of bateriophage T4 , Laemmli, U. K. , Nature / v.227,pp.680-685, 1970
  8. Cross-linked collagen surface for cell culture that is stable, uniform, and optically superior to conventional surfaces , Macklis, J.;R. L. Sidman;D. H. Shine , In Vitro Cell. Dev. Biol. / v.21,pp.189-194, 1985
  9. Estradiol-17β stimulation of vitellogenin synthesis in primary culture of male rainbow trout hepatocytes , Maitre, J.-L.;Y. Valotaire;C. Guguen-Guillouzo , In Vitro Cell. Dev. Biol. / v.22,pp.337-343, 1986
  10. Recent developments in the measurement of nucleic acids in biological materials , Munro, H. N.;A. Fleck , Analyst / v.91,pp.78-88, 1966
  11. Hepatocyte adhesion on plastic , Neumeier, R.;W. Reutter , Exp. Cell Res. / v.160,pp.287-296, 1985
  12. Yolk formation and differentiation in teleost fishes , Ng, T. B.;D. R. Idler;Hoar W. S.(ed.);Randall D. J.(ed.);Donaldson E. M.(ed.) , Fish Physiology / v.9,pp.374-404, 1983
  13. Synthesis of vitellogenin by eel (Angulla anguilla L.) hepatocytes in primary culture : Requirement of 17β-estradiol priming , Peyon, P.;S. Baloche;E. B. Gerard , Gen. Comp. Endocrinol. / v.91,pp.318-329, 1993
  14. Effects of fibronectin-related peptides on cell spreadin , Silnutzer, J. E.;D. W. Barnes , In Vitro Cell. Dev. Biol. / v.21,pp.73-78, 1985
  15. An estrogen responsive primary amphibian liver cell culture system , Stanchfield, J. E.;J. D. Yager , Exp. Cell Res. / v.116,pp.239-252, 1978
  16. Steroid-induced synthesis of vitellogenin in the catfish, Heteropneustes fossilis (Bloch) , Sundararaj, B. I.;P. Nath , Gen. Comp. Endocrinol. / v.43,pp.201-219, 1981
  17. Estrogen and estradiol participation during exogenous vitellogenesis in the female rainbow trout, Salmo gairdneri , Van Bohemen;Ch. G.;J. G. D. Lambert;H. J. Th. Goos;P. G. W. J. Van Oordt , Gen. Comp. Endocrinol. / v.46,pp.81-92, 1982
  18. Vitellogenin and oocyte-growth in nonmammalian vertebrates , Wallace, R. A.;Browder L.(ed.) , Developmental Biology / v.1,pp.127-177, 1985
  19. RNA-DNA ratio as an index to growth in salmonid fishes in the laboratory and in streams contaminated by carbaryl , Wilder, I. B.;J. G. Stanley , J. Fish Biol. / v.22,pp.165-172, 1983
  20. Interactions of cyclic adenosine 3',5'-Monophosphate, protein kinase-C, and calcium in dopamine- and gonadotropin-releasing hormone-stimulated growth hormone release in the goldfish , Wong, A. O. L.;F. V. Goor;R. M. Jobin;C. M. Neumann;J. P. Chang , Endocrinology / v.135,pp.1593-1604, 1994
  21. Effects of extracellular calcium concentrations and calcium antagonists on vitellogenin induction by estradiol-17β in primary hepatocyte culture in the rainbow trout Oncorhynchus mykiss , Yeo, I.-K.;Y. Mugiya , Gen. Comp. Endocrinol. / v.105,pp.294-301, 1997

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

DOI 인용 스타일