Bamboo salt has been used for the purpose of prevention and treatment of various diseases in Korea. Present study was carried out to ascertain the effects of purple bamboo salt upon anti-allergic effect, anti-inflammatory activity and immune-enhance effect as well. Purple bamboo salt significantly i...
Bamboo salt has been used for the purpose of prevention and treatment of various diseases in Korea. Present study was carried out to ascertain the effects of purple bamboo salt upon anti-allergic effect, anti-inflammatory activity and immune-enhance effect as well. Purple bamboo salt significantly inhibited the ear swelling response and histamine release induced by compound 48/80 in mice and rat peritoneal mast cells. Purple bamboo salt (0.01 ∼ lg/kg) also dose-dependently inhibited the passive cutaneous anaphylaxis by oral administration. Purple bamboo salt (1 mg/mL) in hibited phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-1${\beta}$ and IL-6 secretion, by 67.04${\pm}$0.08%, 68.01${\pm}$1.85%, 69.48${\pm}$0.54%, respectively. In addition, purple bamboo salt inhibited the expression of TNF-${\alpha}$ mRNA in HMC-1 cells. Finally, we investigated the effect of purple bamboo salt in the forced swimming test (FST) and the change of purple bamboo salt-mediated cytokine production from MOLT-4 cells. At the 7th, immobility time was significantly decreased in the purple bamboo salt-administration group (35.4 ${\pm}$5.9 s for 1 g/kg) in comparison with the control group (93.2 ${\pm}$ 15.45). After FST, the content of glucose in the blood serum was increased and the levels of blood urea nitrogen, lactic dehydrogenase was decreased in purple bamboo salt-administration group. However, it had no effect on the elevation of CK and TP level. Purple bamboo salt (1 mg/mL) significantly increased the interferon (IFN)-${\gamma}$ and IL-2 level compared with media control (about 3.7-fold for IFN-${\gamma}$, about 3.5-fold for IL-2, p〈0.05) but did not affect the IL-4.
Bamboo salt has been used for the purpose of prevention and treatment of various diseases in Korea. Present study was carried out to ascertain the effects of purple bamboo salt upon anti-allergic effect, anti-inflammatory activity and immune-enhance effect as well. Purple bamboo salt significantly inhibited the ear swelling response and histamine release induced by compound 48/80 in mice and rat peritoneal mast cells. Purple bamboo salt (0.01 ∼ lg/kg) also dose-dependently inhibited the passive cutaneous anaphylaxis by oral administration. Purple bamboo salt (1 mg/mL) in hibited phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-1${\beta}$ and IL-6 secretion, by 67.04${\pm}$0.08%, 68.01${\pm}$1.85%, 69.48${\pm}$0.54%, respectively. In addition, purple bamboo salt inhibited the expression of TNF-${\alpha}$ mRNA in HMC-1 cells. Finally, we investigated the effect of purple bamboo salt in the forced swimming test (FST) and the change of purple bamboo salt-mediated cytokine production from MOLT-4 cells. At the 7th, immobility time was significantly decreased in the purple bamboo salt-administration group (35.4 ${\pm}$5.9 s for 1 g/kg) in comparison with the control group (93.2 ${\pm}$ 15.45). After FST, the content of glucose in the blood serum was increased and the levels of blood urea nitrogen, lactic dehydrogenase was decreased in purple bamboo salt-administration group. However, it had no effect on the elevation of CK and TP level. Purple bamboo salt (1 mg/mL) significantly increased the interferon (IFN)-${\gamma}$ and IL-2 level compared with media control (about 3.7-fold for IFN-${\gamma}$, about 3.5-fold for IL-2, p〈0.05) but did not affect the IL-4.
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가설 설정
1)Purple bamboo salt dissolved with distilled water was orally administered to mice 1 h before the challenge.
제안 방법
However, its possible pharmacological mechanism has not been investigated clearly. This report describes an effect of purple bamboo salt on mast cellmediated immediate-type allergic reactions, inflammatory activity and also immune-enhance effect.
Total RNA was isolated from HMC-1 cells with Easy-blue according to the manufacturer's instruction, PCR was performed with following primers for TNF- a (5CGG GAC GTG GAG CTG GCC GAG GAG3, ; 5CAC CAG CTG GTT ATC TCT CAG CTC 39. The actin (5, GTG GGG CGC CCC AGG CAC CA*; 5, GTC CTT AAT GTC ACG CAC GAT TTC39 was used to verify that equal amounts of RNA were used for reverse transcription and PCR amplification from different experimental conditions.
대상 데이터
Purple bamboo salt (Sambou purple bamboo salt) was provided by Tae Sung Food Inc. (Jeonbuk, South Korea). It was processed by special technique (9 times processing at very high temperature with bay salt, bamboo, pine tree firewood, pine resin, and yellow earth etc.
데이터처리
of experiments. Statistical significance was compared between each treated group and control by the Students t-test. Results with p<0.
이론/모형
Cell viability was determined by the MTT assay. Briefly, 500 UL of HMC-1 cells suspension (2.
Culture supernatants were assayed for each cytokines levels by ELISA method. As shown in Table 3, purple bamboo salt inhibited the secretion of TNF- a, ILTB, and Ⅱ.
h. The levels of IFN- 7, IL-2 and IL-4 were analyzed by ELISA method. As shown in Fig.
mg/mL), purple bamboo salt (1 mg/mL). The protein extracts were prepared and samples were analyzed for IFN-7 expression by western blotting as described method (A). IFN- r levels were quantitated by densitometry (B).
성능/효과
1)Purple bamboo salt or NaCI (1 g/kg for 7 days) was administered orally to mice, we have measured the levels of Glc, LDH, BUN, CK and TP in the serum (n=5). Each level was determined by the autoanalyzer.
8 s). After 7 days, the immobility time was significantly decreased in the purple bamboo saltadministration group (35.4±5.9 s for 1 g/kg) in comparison with the control group (93.2±15.4 s). Our results suggest that the decrease in the immobility time caused by purple bamboo salt administration in the FST might be mediated through immune-enhancement.
We measured the immobility time at the 2, 7days after the administration of saline, NaCl, purple bamboo salt. As a result of test, the immobility time was decreased in the purple bamboo salt-administrated group in a comparison with the saline-administrated group (Fig. 4). After 2 days, the immobility time was decreased in the purple bamboo salt-administration group (68.
It is conceivable that purple bamboo salt inhibits the initial phase of immediate type allergic reactions, probably through interference with the degranulation system. In conclusion, the results obtained in the present study provide evidence that purple bamboo salt inhibited the immediate-type allergic reactions in vivo and in vitro in a murine model.
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