[논문]개심장사상충의 정기 출현성에 관한 정량적 분석

박선일; 이홍수; 안소저; 정석영; 김두

개심장사상충의 정기 출현성에 관한 정량적 분석
Quantitative Analysis of the Periodicity of Dirofilaria immitis in Dogs with Various Microfilarial Densities 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.22 no.2 = no.49, 2005년, pp.79 - 83

박선일 (강원대학교 수의학과) , 이홍수 (강원대학교 수의학과) , 안소저 (강원대학교 수의학과) , 정석영 (강원대학교 수의학과) , 김두 (강원대학교 수의학과)

초록
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본 연구에서는 주거환경이 다른 6두의 개를 대상으로 일정한 시간간격으로 채혈하여 만든 염색표본에서 필라리아자충의 수를 측정하여 정기 출현성을 파악하고자 trigonometric모형을 사용하였다. 모형에 근거하여 계산된 주기성 지수 (periodicity index)는 평균 $57.6\%$ (범위: $25.6-95.5\%$)로 나타났다. 혈액 내 필라리아자충의 수가 최고에 이르는 시점은 21:00 (범위: 20:04-21:29), 최저에 이르는 시점은 09:00 (범위: 08:04-09:29)로 나타났다. 실체로 관찰된 자충의 수와 모형에 의하여 계산된 기대치와의 상관계수는 $6.4-49.2\%$로 자충의 수에 따라 차이를 보였다. 본 연구에 사용된 6두의 실험견 모두 nocturnally sub-periodic 양상을 보였다. 이러한 결과는 기존의 연구에서 보고된 혈중 자충의 수가 최대에 도달하는 시간은 동일하였지만 최저에 이르는 시간은 차이가 있는 것으로 나타났다 국내 개에서 조사 대상지역에 따른 정기 출현성에 차이가 없는지를 명확히 증명하고 심장사상충의 역동성을 보다 정확하게 이해하기 위해서는 광범위한 조사가 필요할 것으로 사료된다.

Abstract ▼ AI-Helper

Six dogs including 4 dogs living in different geographic locations of Kangwon province and 2 client-owned dogs were used to determine the periodicity of microfilariae of D. immitis using a trigonometric model. The calculated periodicity index was ranged from 25.6 to $95.5\%$ with mean of $57.6\%$, and the estimated hour of peak was approximately 21:00 hrs (range, 20:04-21:29 hrs) and minimum counts at 09:00 hrs (range, 08:04 - 09:29 hrs). Correlation coefficient between the observed and the expected count from the model varies depending on dogs, ranging from 6.4 to $49.2\%$. Based on this study, the periodicity of microfilariae of D. immitis was considered as nocturnally sub-periodic for all dogs employed. This result is in consistent with previous report in peak hour but different in minimal hour, indicating that further studies on the periodicity need to be performed to better understanding the dynamics of the periodicity and to help practitioners in the choice of the time for examination of the dogs.

주제어

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제안 방법

immitis infection is different. For better understanding of geographic variations of the microfilariae in Korea we analyzed the periodicity in the peripheral blood of infected dogs living in different environ mental conditions, using a mathematical model.
The dogs were screened by an ELISA kit (Snap™, IDEXX, USA) and microscopic test using plain microhematocrit capi llary tubes (Chase Scientific, USA). Briefly, for antigen positive dogs, the tube was centrifuged for 3 min at 1000 rpm and then observed over the upper side of the bufify coat to detect microfilariae.

데이터처리

347xDxcos 15where Y is the expected microfilarial count at the hour H, D is the difference of density between the peak hour and the mean (called periodicity index by Sasa and Tanaka"), and K is the estimated ho!* of maximum micro filarial density over 24 h. Pearson's correlation coefficient was used to test for the degree of relationship between the observed microfilarial counts and the expected density of microfilariae estimated from the forma. The Statistical Analysis System Version 8.

성능/효과

Previous studies have revealed that different forms exist even within the same filarial species25. Furthermore, the correlation coefficient (range, 6.4-49.2%) in this study was not significantly different between observed and expected counts, indicating that variation in the observed counts may not be explained by the expected micro- filarial counts. Similar findings were reported by Church et al, who reported correlation coefficients of 4.
In conclusion, although the periodicity of D. immitis used in this study has shown great variations depending on the dogs, nocturnal sub-periodic nature of microfilaremia was seen for all dogs. Further studies on the periodicity, which seems to vary in different geographic locations, need to be performed to better understanding the dynamics of the periodicity and to help practitioners in the choice of the time for examination of the dogs.

후속연구

immitis used in this study has shown great variations depending on the dogs, nocturnal sub-periodic nature of microfilaremia was seen for all dogs. Further studies on the periodicity, which seems to vary in different geographic locations, need to be performed to better understanding the dynamics of the periodicity and to help practitioners in the choice of the time for examination of the dogs.

참고문헌 (26)

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