[논문]흰쥐의 중성지질 대사에 미치는 치커리 추출물의 영향

차재영; 박채규; 강호영; 조영수

doi:10.5352/jls.2005.15.4.613

흰쥐의 중성지질 대사에 미치는 치커리 추출물의 영향
Effect of Chicory Extract on Triglyceride Metabolism in Rats 원문보기

생명과학회지 = Journal of life science, v.15 no.4 = no.71, 2005년, pp.613 - 618

차재영 (동아대학교 응용생명공학부) , 박채규 (KT&G 중앙연구소) , 강호영 (부산대학교 생명과학부) , 조영수 (동아대학교 응용생명공학부)

초록
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치커리 수용성 추출물을 $2\%$ 및 $4\%$ 수준으로 첨가한 식이를 Sprague-Dawle계 흰쥐에 2주간 투여하여 중성지질 대사에 미치는 영향을 검토하였다. 혈청 중성지질 농도는 대조군에 비해 치커리 추출물 투여군에서 현저히 감소하였다. Apolipoprotein B-함유 lipoprotein의 합성 및 분비에 필수적인 간장 microsomal triglyceride transfer protein (MTP)활성도 치커리 투여군에서 현저히 감소하였다. 간 조직에서 중성지질 합성의 중요 조절효소로 알려진 phosphatidate phosphohydrolase (PAP) 활성은 각 실험군간에 큰 차이는 없었다. 또한 혈청 및 간 조직의 콜레스테롤, 인지질, 유리지방산 농도도 각 실험군간에 유의적인 차이는 없었다. 이상의 실험결과에서 치커리 수용성 추출물 $2\%$ 및 $4\%$ 첨가는 간 조직에서 혈중으로 분비하는데 중요한 역할을 하는 MTP활성의 감소가 혈청중성지질 농도의 감소에 영향을 미치는 중요한 요인으로 사료되었다.

Abstract ▼ AI-Helper

We investigated the effect of chicory (Chicorium intybus) extract on triglyceride concentration and microsomal triglyceride transfer protein (MTP) activity in rats. The effect of water-soluble extract of chicory fed at the $2.0\%\;and\;4.0\%(w/w)$ levels for 2 weeks on the concentration of serum triglyceride and the activity of hepatic microsomal riglyceride transfer protein (MTP) was investigated in male Sprague-Dawley rats. The triglyceride concentrations in serum of the chicory extract fed groups were significantly lower than in the control group. MTP activity, known to be essential for the assembly/secretion of apolipoprotein B-containing lipoproteins, was also significantly lower in the chicory extract groups than in the control group. The concentrations of other lipids in serum and liver and the activity of phosphatidate phosphohydrolase, the rate-limiting enzyme in triglyceride synthesis, showed no significant differences among in the chicory fed groups. These results indicate that dietary chicory extract decrease hepatic MTP activity and serum triglyceride concentration, and therefore reduces hepatic lipoprotein assembly and secretion.

주제어

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가설 설정

The concentrations of serum lipids are shown in Table 3. The concentrations of triglyceride in the chicory groups were significantly lower than in the control group. This triglyceride-lowering effect of chicory extracts was more pronounced in the high consumption chicory-feeding group.

제안 방법

We have reported that both roasted and unroasted chicory extracts at the levels of 5% (w/w) reduces serum triglyceride concentration in rats and this effect was more pronounced in the unroasted chicory feeding rats compared with the roasted chicory feeding rats[8]. Therefore, in this study we investigated the effect for lower supplemented concentration of chicory extracts on boh serum triglyceride concentration and hepatocyte MTP activity. Our results show a definite correlation between the activity of MTP in liver and the concentration of triglyceride in serum.

데이터처리

All values are presented as means+SE. Data were analyzed using one way analysis of variance (ANOVA), and differences were analyzed using Duncan's new multiple-range test[7]. Statistical significance was accepted when ^<0.

성능/효과

The concentrations of total cholesterol HDL-cholesteroL phospholipid and non-esterified fatty acid in serum were comparable in the chicory groups and the control group. The concentrations of triglyceride, cholesterol and phospholipid in liver are shown in Table 4. Liver lipids did not differ significantly between the control group and the chicory groups.
In conclusion, the present study indicates that dietary roasted-chicory reduces hepatic MTP activity, and thus, serum triglyceride concentration in rats.

참고문헌 (31)

Bartlett, G. R. 1959. Colorimetric assay methods for free and phosphorylated glyceric acids. J Biol Chem. 234, 469-471
Cha, J. Y, Y. S. Cho, I. Kim I, T. Anno, S. M. Rahman, T. Yanagita. 2001. Effects of hesperetin, a citrus flavonoid, on the liver triglyceride content and phosphatidate phosphohydrolase activity in orotic acid-fed rats. Plant Food Human Nutrition 56, 349-358

상세보기
Cha, J. Y., Y. Mameda, K Oogami, K. Yamamoto, T. Yanagita. 1998. Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhanced phosphatidate phosphohydrolase activity in rats. Biosci Biotechnol Biochem 62, 508-513

상세보기
Cha, J. Y., Y. S. Cho. 2000. Effect of potato polyphenolics on the hyperlipidemia in rats. J Korean Sco Food Sci Nutr 29, 274-279
Chang, B. H., W. Liao, L. Li, M. Nakamuta, D. Mack, L. Chan. 1999. Liver-specific inactivation of the abetalipoproteinemia gene completely abrogates very low density lipoprotein/low density lipoprotein production in a viable conditional knockout mice. J Biol Chem 274, 6051-6055

상세보기
Daubioul, C. A, H. S. Taper, L. D. De Wispelaere, N. M. Deizenne. 2000. Dietary oligofructose lessens hepatic steatosis, but dose not prevent hypertriglyceridemia in obese zucker rats. J Nutr 130, 1314-1319

상세보기
Duncan, D. B. 1957. Multiple range test for correlated and heteroscedastic means. Biometrics 13, 164-176

상세보기
Fiordaliso, M., N. Kok, J. P. Desager, F. Goethals, D. Deboyser, M. Roberfroid, N. Delzenne. 1995. Dietary oligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low density lipoproteins of rats. Lipids 30, 163-167

상세보기
Fletcher, M. J. 1968. A colorimetric method for estimating serum triglyceride. Clin Chim Acta 22, 393-397

상세보기
Folch, J., M. Lees, G. H. Sloane-Starley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J BioI Chem 226, 497-509
Fremont, L., M. T. Gozzelino. 1996. Dietary sunflower oil reduces plasma and liver triacylglycerols in fasting rats and is associated with decreased liver microsomal phosphatidate phosphohydrolase activity. Lipids 31, 871-878

상세보기
Gordon, D. A., H. Jamil, D. Sharp, D. Mullaney, Z. Yao, R. E. Gregg, J. Wettwrau. 1994. Secretion of apolipoprotein B containing lipoproteins from HeLa cells is dependent on expression of microsomal triglyceride transfer protein and is regulated by lipid availability. Proc. NatI Acad USA 91, 7628-7632
Jamil, H., J. K Dickson, C. H. Chu, M. W. Lao, J. K. Rinehart, S. A Biller, R. E. Gregg, J. R. Wetterau. 1995. Microsomal triglyceride transfer protein. Specificity of lipid binding and transport. J Biol Chem 270, 6549-6554

상세보기
Kim, M., H. K. Shin. 1998. The water soluble extract of chicory influences serum and liver lipid concentrations, cecal short-chain fatty acid concentrations and fecal lipid excretion in rats. J Nutr 128, 1731-1736

상세보기
Kok, N. N., H. S. Taper, N. M. Delzenne. 1998. Oligofructose modulates lipid metabolism alterations induced by a fat-rich diet in rats. J Appl Toxicol 18, 47-53

상세보기
Kok, K, M. Roberfroid, N. Delzenne. 1996. Dietary oligofructose modifies the impact of fructose on hepatic triacylglycerol metabolism. Metabolism 45, 1547-1550

상세보기
Kok, N. N., L. M. Mordan, C. M. Williams, M. B. Roberfroid, J. P. Thissen, N. M. Delzenne. 1998. Insulin, glucagonlike peptide 1, glucose-dependent insulinotropic polypeptide and insulin-like growth factor I as putative mediators of the hypolipidemic effect of oligofructose in rats. J Nutr 128, 1099-10103

상세보기
Lamb, R. G., H. J. Fallon. 1974. Glycerolipid formation from sn-glycerol-3-phosphate by rat liver cell fractions. Biochim Biophys Acta 348, 166-178

상세보기
Lin, M. C. A, C. Arbeery, K. Bergquist, B. Kienzle, D. A Gordon, J. R. Wetterau. 1994. Cloning and regulation of hamster microsomal triglyceride transfer protein: the regulation is independent from that of other hepatic and transport of fatty acids and triglycerides. J BioI Chem 269, 29138-29145
Livesey, G., T. Smith, B. O. Eggum, I. H. Tetens, M. Nyman, M. Roberfroid, N. Delzenne, T. F. Schweizer, J. Decombaz. 1995. Determination of digestible energy values and fermentabilities of dietary fibre supplements; a European interlaboratory study in vivo. Br J Nutr 74, 289-302

상세보기
Loo, V. J., P. Coussement, L. De Leenheer, M. Hoebregs, G. Smith. 1995. On the presence of inulin and oligofructose as natural ingredients in the Western diet. Crit Rev Food Sci Nutr 35, 525-552

상세보기
Lowry, O. H., N. J. Rosebrough, A. L. Farr, R. S. Randall. 1951. Protein measurement with the Folin phenol reagent. J BioI Chem 193, 265-275
Park, C. K., J. Y. Cha, B. S. Jeon, N. M. Kim, K. H. Shim. 2000. Effects of chicory root water extracts on serum triglyceride and microsomal triglyceride transfer protein (MTP) activity in rats. J Korean Soc Food Sci Nutr 29, 518-524
Raabe, M., M. M. Veniant, M. A. Sullivan, C. H. Zlot, J. Bjorkegren, L. B. Nielsen, I. S. Wong, R. L. Hamilton, S. G. Young. 1999. Analysis of the role of microsomal triglyceride trnsfer protein (MTP) in the liver of tissue-specific knockout mice. J Clin Invest 103, 1287-1298

상세보기
Sperry, W. M., M. Webb. 1950. Averision of the ShoenheimerSperry method for cholesterol determination. J BioI Chem 187, 97-106
Tietge, U. J., A. Bakillah, C. Maugeais, K. Tsukamoto, M. Hussain, D. J. Rader. 1999. Hepatic overexpression of microsomal triglyceride trnsfer protein (MTP) results in increased in vivo secretion of VLDL triglycerides and apolipoprotein B. J Lipid Res 40, 2134-2139

상세보기
Tsuji, Y., K. Yamada, N. Hosoya, S. Moriuchi. 1986. Digestion and absorption of sugars and sugar substitutes in rat small intestine. J Nutr Sci Vitaminol 32, 93-100

상세보기
Vigne, J. L., D. Lairon, P. Borel, H. Portugal, A. M. Pauli, J. C. Hautori., H. Lafont. 1987. Effect of pectin, wheat bran and cellulose on serum lipids and lipoproteins in rats fed on a low- or high-fat diet. Br J Nutr 58, 405-413

상세보기
Walton, P. A., F. Possmayer. 1984. The role of Mg2+-dependent phosphatidate phosphohydrolase in pulmonary glycerolipid biosynthesis. Biochim Biophys Acta 796, 346-372
Wang, Y. M., S. M. Rahman, K. Nagano, et al. 2003. Conjugated linoleic acid reduces hepatic microsomal triacylglycerol transfer protein activity and hepatic triacylglycerol mass in obese rats. J Oleo Sci 52, 129-134

상세보기
Wetterau, J. R., L. P. Aggerbeck, M. E. Bouma, C. Eisenberg, A. Munck, M. Hermier, J. Schmizt, G. Gay, D. J. Rader, R. E. Gergg. 1992. Absence of microsomal triglyceride transfer protein in individuals with abetalipoproteinemia. Science 258, 999-1001

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