Aspergilus Oyzae 혹은 Saccharomyces Cerevisiae를 첨가하여 제조한 맥주박 위주 발효사료가 반추위 미생물에 미치는 영향 Effects of Brewery Meal-Based Fermented Feedstuff Supplemented with Aspergillus Oryzae or Saccharomyces Cerevisiae on Ruminal Microorganism원문보기
본 연구는 Aspergillus oryzae(AO) 혹은 Saccharomyces cerevisiae(SC)를 첨가하여 제조한 맥주박 위주 발효사료의 급여가 한우의 반추위내 미생물에 미치는 영향을 조사하기 위해 실시하였다. 공시동물은 반추위 cannula가 장착된 한우 암소 2두를 이용하였다. 시험은 시판 배합사료 $715\%$ 및 corn silage $28.5\%$ 급여구(대조구), 시판 배합사료 $45.0\%$, AO 첨가 발효사료 $26.5\%$ 및 com silage $28.5\%$ 급여구(TAO)와 시판 배합사료 $45.0\%$, SC 첨가 발효사료 $26.5\%$ 및 corn silage $28.5\%$ 급여구(TSC)의 3처리구로 구분하여 수행하였다. Total viable bacteria(p<0.05), 혐기성 곰팡이 및 protozoa(p<0.05)의 수는 대조구에 비해 TAO 및 TSC에서 높았다. 단백질 분해 박테리아(p<0.05), 섬유소 분해 박테리아 및 xylan 이용 박테리아 수는 대조구에 비해 TAO 및 TSC구에서 높은 경향이었다 Protozoa의 건물회수율은 대조구에 비해 TAO 및 TSC구에서 높았다(p<0.05). 총 미생물 및 protozoa의 조단백질 함량은 대조구 및 TAO구에 비해 TSC구에서 높았다(p<0.05). 박테리아의 조단백질 함량은 대조구에 비해 TAO 및 TSC구에서 높았다(p<0.05). 총 미생물의 조지방 함량은 대조구 및 TSC구에 비해 TAO구에서 높았으며 (p<0.05), protozoa 및 박테리아의 조지방 함량은 대조구 및 TAO구에 비해 TSC구에서 높았다(p<0.05). 총 미생물의 필수아미노산 비율은 TAO 및 TSC구에 비해 대조구에서 높았다. 박테리아의 methionin 및 alanine 비율은 대조구에 비해 TAO 및 TSC구에서 높았다(p<0.05). 이상의 결과에서 AO 혹은 SC를 첨가하여 제조한 발효사료의 급여는 반추위내 미생물 수 및 균체 조성 변화에 영향을 미치는 것으로 판단된다.
본 연구는 Aspergillus oryzae(AO) 혹은 Saccharomyces cerevisiae(SC)를 첨가하여 제조한 맥주박 위주 발효사료의 급여가 한우의 반추위내 미생물에 미치는 영향을 조사하기 위해 실시하였다. 공시동물은 반추위 cannula가 장착된 한우 암소 2두를 이용하였다. 시험은 시판 배합사료 $715\%$ 및 corn silage $28.5\%$ 급여구(대조구), 시판 배합사료 $45.0\%$, AO 첨가 발효사료 $26.5\%$ 및 com silage $28.5\%$ 급여구(TAO)와 시판 배합사료 $45.0\%$, SC 첨가 발효사료 $26.5\%$ 및 corn silage $28.5\%$ 급여구(TSC)의 3처리구로 구분하여 수행하였다. Total viable bacteria(p<0.05), 혐기성 곰팡이 및 protozoa(p<0.05)의 수는 대조구에 비해 TAO 및 TSC에서 높았다. 단백질 분해 박테리아(p<0.05), 섬유소 분해 박테리아 및 xylan 이용 박테리아 수는 대조구에 비해 TAO 및 TSC구에서 높은 경향이었다 Protozoa의 건물회수율은 대조구에 비해 TAO 및 TSC구에서 높았다(p<0.05). 총 미생물 및 protozoa의 조단백질 함량은 대조구 및 TAO구에 비해 TSC구에서 높았다(p<0.05). 박테리아의 조단백질 함량은 대조구에 비해 TAO 및 TSC구에서 높았다(p<0.05). 총 미생물의 조지방 함량은 대조구 및 TSC구에 비해 TAO구에서 높았으며 (p<0.05), protozoa 및 박테리아의 조지방 함량은 대조구 및 TAO구에 비해 TSC구에서 높았다(p<0.05). 총 미생물의 필수아미노산 비율은 TAO 및 TSC구에 비해 대조구에서 높았다. 박테리아의 methionin 및 alanine 비율은 대조구에 비해 TAO 및 TSC구에서 높았다(p<0.05). 이상의 결과에서 AO 혹은 SC를 첨가하여 제조한 발효사료의 급여는 반추위내 미생물 수 및 균체 조성 변화에 영향을 미치는 것으로 판단된다.
This study was conducted to examine effects of brewery meal-based fermented feedstuff supplemented with Aspergillus oryzae(AO) or Saccharomyces cerevisiae(SC) on luminal micro-organism of Korean native cattle. Two cows equipped with luminal cannulas were used as experimental animals. Experiment was ...
This study was conducted to examine effects of brewery meal-based fermented feedstuff supplemented with Aspergillus oryzae(AO) or Saccharomyces cerevisiae(SC) on luminal micro-organism of Korean native cattle. Two cows equipped with luminal cannulas were used as experimental animals. Experiment was done with three treatment groups: $71.5\%$ of commercial feed and $28.5\%$ of com silage(control): $45.0\%$ of commercial feed, $26.5\%$ of fermented feedstuff supplemented with AO and $28.5\%$ of corn silage(TAO): $45.0\%$ of commercial feed, $26.5\%$ of fermented ffedstuff supplemented with SC and $28.5\%$ of corn silage(TSC). The number of total viable bacteria (p<0.05), anaerobic fungi and protozoa(p<0.05) was higher in TAO and TSC than in control. The number of proteolytic bacteria(p<0.05), cellulolytic bacteria and xylan fermenters tended to be higher in TAO and TSC than in control. The dry matter recovery (DMR) of protozoa was higher in TAO and TSC than in control(p<0.05). The crude protein (CP) content of total microbes and protozoa was higher in TSC than in control and TAO (p<0.05). The CP content of bacteria was higher in TAO and TSC than in control(p<0.05). The ether extract(EE) content of the total microbes was higher in TAO than in control and TSC(p<0.05), and the EE of protozoa and bacteria were higher in TSC than in control and TAO(p<0.05). The ratio of essential amino acids of total microbe was higher in control than in TAO and TSC(p<0.05). The ratio of methionine and alanine of bacteria was higher in TAO and TSC than in control(p<0.05). The results suggested that the feeding of fermented feedstuff supplemented with AO or SC had an influence on the numbers of ruminal microorganism and the changes of microbial body composition.
This study was conducted to examine effects of brewery meal-based fermented feedstuff supplemented with Aspergillus oryzae(AO) or Saccharomyces cerevisiae(SC) on luminal micro-organism of Korean native cattle. Two cows equipped with luminal cannulas were used as experimental animals. Experiment was done with three treatment groups: $71.5\%$ of commercial feed and $28.5\%$ of com silage(control): $45.0\%$ of commercial feed, $26.5\%$ of fermented feedstuff supplemented with AO and $28.5\%$ of corn silage(TAO): $45.0\%$ of commercial feed, $26.5\%$ of fermented ffedstuff supplemented with SC and $28.5\%$ of corn silage(TSC). The number of total viable bacteria (p<0.05), anaerobic fungi and protozoa(p<0.05) was higher in TAO and TSC than in control. The number of proteolytic bacteria(p<0.05), cellulolytic bacteria and xylan fermenters tended to be higher in TAO and TSC than in control. The dry matter recovery (DMR) of protozoa was higher in TAO and TSC than in control(p<0.05). The crude protein (CP) content of total microbes and protozoa was higher in TSC than in control and TAO (p<0.05). The CP content of bacteria was higher in TAO and TSC than in control(p<0.05). The ether extract(EE) content of the total microbes was higher in TAO than in control and TSC(p<0.05), and the EE of protozoa and bacteria were higher in TSC than in control and TAO(p<0.05). The ratio of essential amino acids of total microbe was higher in control than in TAO and TSC(p<0.05). The ratio of methionine and alanine of bacteria was higher in TAO and TSC than in control(p<0.05). The results suggested that the feeding of fermented feedstuff supplemented with AO or SC had an influence on the numbers of ruminal microorganism and the changes of microbial body composition.
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문제 정의
Therefore, various researches have reported that dietary AO or SC causes a range of effects on ruminal microorganism, but, to our knowledge, no data are available on the eflect of fermented feedstuff supplemented with AO or SC on ruminal microorganism. Thus, the objective of this study was to evaluate the effects of brewery meal-based fermented feedstuff supplemented with AO or SC on ruminal microorganism and their body composition.
제안 방법
Two cows equipped with ruminal cannulas were used as experimental animals. Experiment was done with three treatment groups: 71.5% of commercial feed and 28.5% of com silage(control); 45.0% of commercial feed, 26.5% of fermented feedstuff supplemented with AO and 28.5% of corn silage(TAO); 45.0% of commerci사 feed, 26.5% of fermented feedstuff supplemented with SC and 28.5% of com silage(TSC). The number of total viable bacteria (p<0.
For examination of the changes of ruminal microbi지 body compositions, animals were first adapted for 14 days on experimental diets and the experimental period lasted for 3 days, and 200 ml of ruminal fluid and 200 g of ruminal digesta were collected into 100 ml plastic bobles at 3, 6, 9 and 12 hours after the morning feeding during the first day, at 1, 4, 7 and 10 hours after the morning feeding during the second day, and 2, 5, 8 and 11 hours after the morning feeding during the third day. This sequence of sampling allowed a sample to be taken every hour during 12 hours.
This study was conducted to examine effects of brewery meal-based fermented feedstuff supplemented with Aspergillus oryzae(\O) or Saccharomyces cerevisiae(SC) on ruminal microorganism of Korean native cattle. Two cows equipped with ruminal cannulas were used as experimental animals.
Chemical compositions of experimental diets were shown in Table 1. Treatments were composed of three treatment groups: 71.5% of commercial feed and 28.5% of corn silage(control); 45.0% of commercial feed, 26.5% of fermented feedstuff supplemented with AO and 28.5% of corn silage(TAO); 45.0% of commercial feed, 26.5% of fermented feedstuff supplemented with SC and 28.5% of com silage(TSC). The experiments were accomplished in duplicate.
대상 데이터
Two Korean native cattle(400 and 450 kg) equipped with ruminal cannula were used as experimental animals. Experimental diets were formulated using the procedure described by Lin et al.
이론/모형
Data were analyzed by Duncan's procedure using GLM of SAS(1999). The statistical model was as follow;
성능/효과
05). The ether extract(EE) content of the total microbes was higher in TAO than in control and TSC(p<0.05), and the EE of protozoa and bacteria were higher in TSC than in control and TAO(p<0.05). The ratio of essential amino acids of total microbe was higher in control than in TAO and TSC(p<0.
The order of CP content appeared in the sequence of TSC > TAO > control(23.74, 23.30 and 23.15%, respectively) for total microbes, and the order appeared in the sequence of TSC > TAO 그 control(43.30, 43.26 and 42.79%, respectively) for bacteria; thereby, CP content of ruminal microorganism was increased by adding of SC.
05). The ratio of nonessential amino acids(NEAA) of total microbes and protozoa was higher in TSC than in control and TAO (p<0.05), and the ratio of NEAA was also higher in TAO than in control(p<0.05). Also, the ratio of EAA and NEAA of bacteria did not differ among the treatments.
05). The results suggested that the feeding of fermented feedstuff supplemented with AO or SC had an influence on the numbers of ruminal microorganism and the changes of microbial body composition.
후속연구
Furthermore, EE content of protozoa and bacteria were significantly increased by adding of SC, although we can not clarify clear conclusion in this experiment. However, it was presumed by the existence of an unknown factor which activate EE synthesis of ruminal microorganism within SC, and we think as the thing by needing more research about this phenomenon.
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