[논문]배양액 종류, EGF, <tex>${\\beta}-ME$</tex> 및 호르몬이 돼지 난자의 체외 성숙율에 미치는 영향에 관한 연구

장서희; 이만희; 김상근

[국내논문] 배양액 종류, EGF, ${\\beta}-ME$ 및 호르몬이 돼지 난자의 체외 성숙율에 미치는 영향에 관한 연구
Study on Effects of Media, EGF, ${\\beta}-ME$ and Hormones on IVM of Porcine Oocytes 원문보기

韓國受精卵移植學會誌 = Korean journal of embryo transfer, v.21 no.3, 2006년, pp.247 - 253

장서희 (충남대학교 수의과대학) , 이만희 (경북대학교 수의과대학) , 김상근 (충남대학교 수의과대학)

초록
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본 연구는 안정된 돼지 체외 성숙 난자를 얻을 목적으로 배양액의 종류 및 배양액에 EGF, ${\beta}-ME$, 호르몬 첨가가 돼지 난포란의 체외 성숙에 미치는 영향을 조사하였다. 난포란을 TCM-199, NCSU-23 및 PZM-3으로 48시간 배양했을 때 체외 성숙율은 각각 $22.1{\pm}0.70%,\;30.6{\pm}0.70%$ 및 $30.4{\pm}2.82%$였다. TCM-199로 48시간 배양했을 때 체외 성숙율은 NCSU-23 및 PZM-3 보다 약간 낮은 체외 발생율을 나타냈다. 난포란을 25 ng/ml의 EGF를 첨가한 TCM-199, NCSU-23 및 PZM-3로 48시간 배양했을 때 체외 성숙율은 각각 $46.3{\pm}2.8%,\;76.6{\pm}3.1%$ 및 $72.2{\pm}2.6%$로 나타났다. 난포란의 배양 시 배양액에 25 및 50 ng/ml의 EGF를 첨가 후 48시간 배양했을 때 첨가하지 않은 군에 비해 높은 체외 성숙율을 나타냈다(p<0.05). 난포란을 NCSU-23 및 PZM-3 배양액에 $25{\mu}M/ml$의 ${\beta}-ME$를 첨가한 후 48시간 배양했을 때 체외성숙율은 각각 $43.9{\pm}1.41%,\;41.7{\pm}l.41%,\;44.4{\pm}0.70%,\;40.6{\pm}0.70%$로 나타났다. 난포란을 $25{\mu}M/ml$의 ${\beta}-ME$를 첨가한 NCSU-23로 48시간 배양했을 때 첨가하지 않은 군에 비해 높은 체외 성숙율을 나타냈다(p<0.05). 난포란의 배양 시 NCSU-23에 PMSG, hCG, PMSG+hCG, hCG+${\beta}$-estradiol, PMSG+${\beta}$-estradiol을 첨가 후 배양하였을 때 체외 성숙율은 각각 75.6%, 77.8%, 80.0%, 86.4% 및 84.8%로서 무첨가 군(64.4%)에 비해 높게 나타났다(p<0.05).

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제안 방법

In order to determine better conditions for in vitro maturation of pig oocytes, this study also evaluated the arrest/resumption of oocyte matura tion with media, EGF, 3-ME and hormones.
This experiment was conducted to investigate the effects of kinds of media on the IVM rate of porcine oocytes cultured in maturation medium for 0, 24, 48 and 72 hrs, the in vitro maturation rate were shown in Table 1.
This study was investigated in vitro maturation rate of oocytes cultured in maturation media with or without supplementation of EGF, B -ME and hor mones.
This experiment was conducted to investigate the effects of concentration on the IVM rate of oocytes cultured maturation media for 0~72 hrs, the in vitro maturation rate were shown in Fig. 1 and 2.

데이터처리

For comparison of means, Duncan's multiple verification was performed using SAS package of general Linears Model procedures (SAS Institute).

성능/효과

1. When the oocytes were cultured in TCM-199, NCSU-23 and PZM-3 for 48 hrs, the matu ration rates were 25.4±0.70%, 30.4±0.70% and 30.4±2.82%, respectively. The maturation rate of oocytes cultured in TCM-199 was slightly lower than cultured in NCSU-23 and PZM-3 0?<0.
2. The IVM rate of oocytes cultured in TCM- 199, NCSU-23 and PZM-3 supplementation with 25 ng/ml EGF for 48 hrs were 46.3土 2.8%, 76.6±3.1%, 72.2±2.6%, respectively. When the oocytes were cultured in TCM-199, NCSU- 23 and PZM-3 supplemented with 25 ng/ml for 48 hrs, the IVM rate were higher than cultured in none supplementation of EGF (p< 0.
3. The IVM rate of oocytes were cultured in TCM-199, NCSU-23 and PZM-3 supplemen tation with 25 µ M/ml 8-ME for 24 or 48 hrs were 35.8±0.70%, 34.5±1.41%, 35.5±1.41 %, 32.2±0.70% and 43.9±1.41%, 41.7±1.41%, 44.4土0.70%, 40.6±0.70%, respectively. When the oocytes were cultured in TCM-199, NCSU- 23 and PZM-3 supplemented with 25 µ M/ml 8-ME for 48 hrs, the IVM rate were higher than cultured in non supplementation higher of 3-ME 0VO.
4. The IVM rate of oocytes cultured in TCM- 199 with supplementation of MSG, hCG, PMSG +hCG, hCG+ -estradiol, PMSG+ -estradiol for 48 hrs were 75.6%, 77.8%, 80.0%, 86.4% and 84.8%, respectively, and the IVM rate of oocytes cultured in NCSU-23 supplemented with hormones for 48 hrs higher than cultured in non supplementation of hormones (64.4%).

참고문헌 (20)

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