[논문]Isolation of a Pestalotiopsis Species Degrading Mucilage from Fruit of Opuntia ficus-indica var. Saboten

Huh, Yoon-Hee; Ko, Young-Hwan

Isolation of a Pestalotiopsis Species Degrading Mucilage from Fruit of Opuntia ficus-indica var. Saboten 원문보기

Journal of applied biological chemistry, v.50 no.4, 2007년, pp.221 - 226

Huh, Yoon-Hee (Department of Food Bioengineering, Cheju National University) , Ko, Young-Hwan (Department of Food Bioengineering, Cheju National University)

Abstract ▼ AI-Helper

The high molecular-weight mucilage extracted and purified from cactus fruit of Opuntia ficus-indica var. Saboten was degraded by the cell-free culture filtrate of a fungus isolated from soil. TLC analysis of the polymeric mucilage after incubation with the fungal culture filtrate confirmed its degradation. When the degradation products were tested for their qualitative reactions with ninhydrin and phenol-sulfuric acid, only phenol-sulfuric acid gave positive development, and ninhydrin did not show any observable color reaction. This coloring reaction suggested the presence of a carbohydrate without an amino group within the mucilage. Analyses by HPLC and liquid gel permeation chromatography on sephadex G-100 also provided additional information on degradation of the mucilage by the fungal culture filtrate. The sequences of ITS-5.8S rDNA from the fungal isolate that was cultivated for the preparation of mucilage-degrading enzyme showed 99% similarity to those of Pestalotiopsis aquatica.

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문제 정의

No report has yet been published on the microbial enzymatic degradation of the mucilage from the palm-shaped cactus cultivated in Jeju Island. The aim of this study was to isolate a fungus that produces the mucilage-degrading enzyme.

제안 방법

45 pm syringe filter before injection into the HPLC column. HPLC analysis was carried out on a Waters 2690 Millenium32 (Milford, Maryland, USA) apparatus equipped with a Waters 2414 RI detector employing a Phenomenex BIOSEP-SEC- S4000 column (300 x 7.0 mm). Elution was carried out with distilled H₂O.

이론/모형

Carbohydrate contents were determined by the Molisch method [Foulger, 1931], and total sugar contents were determined by the phenol- sulfiiric acid method [Bloor, 1956] using maltose as a standard. Protein contents were determined by the ninhydrin method [Moore and Stein, 1948].
Protein contents were determined by the ninhydrin method [Moore and Stein, 1948].

참고문헌 (16)

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David VJ and Leonard BT (1997) A conserved motif in the 5.8s ribosomal RNA (rRNA) gene is a useful diagnostic marker for plant internal transcribed spacer (ITS) sequences. Plant Mol Biol Rep 15, 326-334

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Foulger JH (1931) The use of the Molisch(alpha-naphthol) reactions in the study of sugars in biological fluids. J Biol Chem 92, 345-353
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Matsuhiro B, Lillo LE, Saenz C, Urzua CC, and Zarate O (2006) Chemical characterization of the mucilage from fruits of Opuntia ficus-indica. Carbohyd Polym 63, 263- 267

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McGarvie D and Parolis H (1981a) Methylation analysis of the mucilage of Opuntia ficus-indica. Carbohyd res 88, 305-314

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McGarvie D and Parolis H (1981b) The acid-labile peripheral chains of the mucilage of Opuntia ficus-indica. Carbohyd Res 94, 57-65

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Stahl E (1969) Thin-layer Chromatography: A laboratory handbook (2nd ed.) pp. 889-900, Toppan Printing Co., New York, USA
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Isolation of a Pestalotiopsis Species Degrading Mucilage from Fruit of Opuntia ficus-indica var. Saboten 원문보기

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Isolation of a Pestalotiopsis Species Degrading Mucilage from Fruit of Opuntia ficus-indica var. Saboten 원문보기

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