[국내논문]A comparison of single dose efficacy of Mycoplasma hyopneumoniae bacterin in swine farms with different serological patterns of PRRSV and PCV2원문보기
This study was to evaluate the efficacy of single dose Mycoplasma hyopneumoniae (M. hyo)-vaccination in the swine farms which had different serological patterns of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2). A minimum of 240 pigs from each farm w...
This study was to evaluate the efficacy of single dose Mycoplasma hyopneumoniae (M. hyo)-vaccination in the swine farms which had different serological patterns of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2). A minimum of 240 pigs from each farm was applied, allocating M. hyo vaccinated and control groups. The PRRSV and PCV2 infections were analyzed by serological method (commercial ELISA kit). After administrating pigs a single dose of M. hyo vaccine or control saline at 3 weeks of age, serum antibodies to M. hyo, PRRSV and PCV2 were monitored at 4, 10, 16 and 22 weeks of age. Mortality, weight changes, feed conversion ratio (FCR) and lung score were also evaluated. A single-dose vaccination of M. hyo bacterin was efficacious to reduce mycoplasmal lung lesions and induce good humoral immune response. However, FCR was improved only in one of the three farms where showed seronegative status to both PRRSV and PCV2 in the period from 4 to 16 weeks of age. These results might imply that M. hyo vaccine alone could not overcome the PRRSV and PCV2 infection-associated wasting in the field condition. Therefore, the control of PRRSV and PCV2 should be considered to obtain the better effects of M. hyo vaccination.
This study was to evaluate the efficacy of single dose Mycoplasma hyopneumoniae (M. hyo)-vaccination in the swine farms which had different serological patterns of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2). A minimum of 240 pigs from each farm was applied, allocating M. hyo vaccinated and control groups. The PRRSV and PCV2 infections were analyzed by serological method (commercial ELISA kit). After administrating pigs a single dose of M. hyo vaccine or control saline at 3 weeks of age, serum antibodies to M. hyo, PRRSV and PCV2 were monitored at 4, 10, 16 and 22 weeks of age. Mortality, weight changes, feed conversion ratio (FCR) and lung score were also evaluated. A single-dose vaccination of M. hyo bacterin was efficacious to reduce mycoplasmal lung lesions and induce good humoral immune response. However, FCR was improved only in one of the three farms where showed seronegative status to both PRRSV and PCV2 in the period from 4 to 16 weeks of age. These results might imply that M. hyo vaccine alone could not overcome the PRRSV and PCV2 infection-associated wasting in the field condition. Therefore, the control of PRRSV and PCV2 should be considered to obtain the better effects of M. hyo vaccination.
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제안 방법
hyo vaccine, especially in daily weight gain and mortality [19], PCV2 infectious status which is the main criteria of PMWS did not included. Therefore, this study was performed to evaluate the field efficacy of the M. hyo vaccine with a single-dose protocol in the three commercial farms, observing the serological status of PRRSV and PCV2 of each farm.
For each experimental group, 10 blood samples were taken randomly from pigs at 4, 10, 16 and 22 weeks of age to monitor serum antibodies to M. hyo, PRRSV and PCV2. In the case of farm A, first blood sampling was performed with pigs at 5 weeks of age.
at 2, 500 rpm for 20 min at 4. An indirect enzyme linked immunosorbent assay kit for M. hyo (ELISA kit, Herdcheck M. hyo; IDEXX Laboratories, USA) was used to evaluate the antibody titers and titers were presented as S/P ratios. For PRRSV and PCV2, commerci시 ELISA kit was also used (PRRSV, Herdcheck PRRS 2XR; IDEXX Laboratories, USA) (PCV2, SEREL1SA PCV2 Ab mono미ocking; Synbiotics Europe, France).
Pearson's chi-square test and Fisher's exact test were performed to statistically analyze the mortality and ratio of lungs with mycoplasmal lesion between two groups. For the comparison of mean feed conversion ratio, mean weight and mean lung score between the two groups, a student's r-test was performed using the SPSS 12.
hyo vaccine should also be estimated in the field condition. Therefore, this study was performed to evaluate the efficacy of commercial M. hyo vaccine in the three commercial Korean swine farms with a one- shot vaccination schedule at 3 weeks of age, observing serologic지 status of PRRSV and PCV2.
With diverse conditions in field context, these criteria could be affected by other disease status, especially PRRSV and PCV2 infections which are associated with pig's wasting in the field. Since dual infection of PRRSV and PCV2 was one of the most potential causes of PMWS among other pathogens [22] , this study was focused on the infectious status of PRRSV and PCV2 to assay the relationship of these viruses with efficacy of M. hyo vaccination, using ser이ogical method.
대상 데이터
Three farrow to finish farms (designated A, B, and C), all of which were located in the Gyeonggi province in Korea, were selected. All farms weaned piglets (crossbreeding, Landrace x Yorkshire x Duroc) at 21 days of age and had been diagnosed to be infected with M.
2. Number of porcine circovirus type 2 (PCV2)- seroconverted pigs among the 20 tested samples of each farm at 4, 10, 16, 22 weeks of age.
데이터처리
The proportion of lungs with mycoplasmal lesion in total investigated lungs was compared in each groups. The independence of two variables(vaccinated or control/lungs with mycoplasmal lesion or not) was analyzed by Pearson's chi-square test using SPSS 12.0.
Pearson's chi-square test and Fisher's exact test were performed to statistically analyze the mortality and ratio of lungs with mycoplasmal lesion between two groups. For the comparison of mean feed conversion ratio, mean weight and mean lung score between the two groups, a student's r-test was performed using the SPSS 12.0 program (SPSS, USA).
성능/효과
hyo vaccination improves pig performance compared to non-vaccinated pigs [4, 6]. In this study, a single-dose vaccination of M. hyo bacterin to the pigs at 3 weeks of age was efficacious to reduce mycoplasmal lung lesions and induce a good humoral immune response. However, mean weight and FCR was not always improved in M.
후속연구
hyo vaccination, especially in mean weight and FCR in this study. As this study did not provide information whether the FCR was affected by PRRSV and PCV2 infection directly or indirectly concerning with other swine pathogens, and did not perform histopathological tests to confirm the PMWS, more study in restricted condition, rather than in the field condition w祖 be needed to s叩port the result of this study.
참고문헌 (25)
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