사람 Y염색체상에 존재하는 RPS4Y(Ribosomal Protein S4Y) 유전자는 성별감정시 유용한 유전자로 규명되어 유전질환의 조기 발견이나 예방 및 태아의 성별판정 등에 응용되고 있다. 신원이 불분명한 사체에서 성별감정시, 기존의 성별감정에 이용되고 있는 다른 유전자와 함께 RPS4Y 유전자를 검색함으로써 성별감정의 신뢰도를 높힐 수 있을 것으로 사료된다. 또한 사체의 손상이 심할 때 유전자를 이용한 개인식별은 제한을 받게 된다. 이때 치아는 인체의 기관 중 가장 견고한 구조로 구성되어 있어 외부 환경에 대한 물리적, 화학적 저항성이 높아 법의치과학적 개인식별에 널리 이용되므로, 본 연구에서는 사람 치아에서 중합효소연쇄반응법을 이용한 RPS4Y유전자를 검출하여 법의학적 성별감정에 응용하고자 하였다. 남녀 각각 10개의 치아에서 치수와 상아질을 분리한 후 DNA를 추출하여 중합효소연쇄반응을 시행하였다. RPS4Y 유전자를 검출한 결과, 남자에게서만 특이적으로 유전자가 검출되었으며, 이는 사람 치아에서 RPS4Y 유전자를 이용한 성별감정이 법의학적 개인식별의 성별감정 실무에 있어서 다른 유전자와 함께 유용하게 사용될 수 있을 것으로 사료된다.
사람 Y염색체상에 존재하는 RPS4Y(Ribosomal Protein S4Y) 유전자는 성별감정시 유용한 유전자로 규명되어 유전질환의 조기 발견이나 예방 및 태아의 성별판정 등에 응용되고 있다. 신원이 불분명한 사체에서 성별감정시, 기존의 성별감정에 이용되고 있는 다른 유전자와 함께 RPS4Y 유전자를 검색함으로써 성별감정의 신뢰도를 높힐 수 있을 것으로 사료된다. 또한 사체의 손상이 심할 때 유전자를 이용한 개인식별은 제한을 받게 된다. 이때 치아는 인체의 기관 중 가장 견고한 구조로 구성되어 있어 외부 환경에 대한 물리적, 화학적 저항성이 높아 법의치과학적 개인식별에 널리 이용되므로, 본 연구에서는 사람 치아에서 중합효소연쇄반응법을 이용한 RPS4Y유전자를 검출하여 법의학적 성별감정에 응용하고자 하였다. 남녀 각각 10개의 치아에서 치수와 상아질을 분리한 후 DNA를 추출하여 중합효소연쇄반응을 시행하였다. RPS4Y 유전자를 검출한 결과, 남자에게서만 특이적으로 유전자가 검출되었으며, 이는 사람 치아에서 RPS4Y 유전자를 이용한 성별감정이 법의학적 개인식별의 성별감정 실무에 있어서 다른 유전자와 함께 유용하게 사용될 수 있을 것으로 사료된다.
Ribosomal Protein S4Y(RPS4Y) gene is the human sex-linked gene on the Y chromosome. There are a number of reports on the sex determination using RPS4Y gene analysis for prevention and diagnosis in sex-linked disease. Thus RPS4Y gene is a reliable genetic marker for sex determination in forensic medi...
Ribosomal Protein S4Y(RPS4Y) gene is the human sex-linked gene on the Y chromosome. There are a number of reports on the sex determination using RPS4Y gene analysis for prevention and diagnosis in sex-linked disease. Thus RPS4Y gene is a reliable genetic marker for sex determination in forensic medicine. In general, the sex determination of an unidentified body can be achieved based on anatomical characteristics, but sometimes sex determination was considered to be difficult such as pre-adolescent bodies or decomposed, mutilated bodies. In this case, Sex determination using PCR method in human teeth produces good results. Because human teeth have a great structural durability, the DNA well preserved in the teeth. So author isolated nuclear DNA from the 20 human teeth(10 males, 10 females), performed to detect RPS4Y gene by PCR method. Samples were divided four group(10 pulp and 10 dentinal tissue in male, 10 pulp and 10 dentinal tissue in female). It was found that detection of RPS4Y gene for sex determination was possible in all the male pulp tissues and 6 out of 10 male dentinal tissues. But there was not detected in female pulp and dentinal tissues. In the view of this results demonstrates the possibility that detection of RPS4Y gene with other sex chromosome genes from the human teeth is useful to sex determination in forensic medicine.
Ribosomal Protein S4Y(RPS4Y) gene is the human sex-linked gene on the Y chromosome. There are a number of reports on the sex determination using RPS4Y gene analysis for prevention and diagnosis in sex-linked disease. Thus RPS4Y gene is a reliable genetic marker for sex determination in forensic medicine. In general, the sex determination of an unidentified body can be achieved based on anatomical characteristics, but sometimes sex determination was considered to be difficult such as pre-adolescent bodies or decomposed, mutilated bodies. In this case, Sex determination using PCR method in human teeth produces good results. Because human teeth have a great structural durability, the DNA well preserved in the teeth. So author isolated nuclear DNA from the 20 human teeth(10 males, 10 females), performed to detect RPS4Y gene by PCR method. Samples were divided four group(10 pulp and 10 dentinal tissue in male, 10 pulp and 10 dentinal tissue in female). It was found that detection of RPS4Y gene for sex determination was possible in all the male pulp tissues and 6 out of 10 male dentinal tissues. But there was not detected in female pulp and dentinal tissues. In the view of this results demonstrates the possibility that detection of RPS4Y gene with other sex chromosome genes from the human teeth is useful to sex determination in forensic medicine.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
문제 정의
The purpose of this study was to evaluate the reliability and possibility of sex determination after detection of RPS4Y gene from the extracted human teeth.
제안 방법
1.5% agarose gel containing Ethidium bromide(0.5 ㎍/㎖), 9 ㎕ of the PCR product and 1 ㎕ of 10X DNA loading buffer(20% Ficoll 400, 0.1 M EDTA pH 8.0, 1% S.D.S., 0.25% Bromphenol Blue, 0.25% Xylene cyanol) was mixed and was performed, subsequently, by using a UV Transilluminator, the size of PCR products was analyzed. As the Marker, 100 bp DNA Ladder(Bioneer, Daegeon, Korea) used.
Author could not find the report to utilize the RPS4Y gene for sex determination in human teeth, and tried to verifying the possibility of sex determination by detection of RPS4Y gene in human teeth. In this study, RPS4Y gene was detected in 10 out of 10 male pulp tissues and 6 out of 10 male dentinal tissues.
대상 데이터
The pulp tissue(10 males, 10 females) and dentinal tissue(10 males, 10 females) from the human teeth were selected for this study. The ages ranged from 19 to 56 years, each male and female averaged age was 29.
성능/효과
Author could not find the report to utilize the RPS4Y gene for sex determination in human teeth, and tried to verifying the possibility of sex determination by detection of RPS4Y gene in human teeth. In this study, RPS4Y gene was detected in 10 out of 10 male pulp tissues and 6 out of 10 male dentinal tissues. All the female sample in this study, 10 pulp tissues and 10 dentinal tissues, were not detected.
It was found that detection of RPS4Y gene for sex determination was possible in all the male pulp tissues and 6 out of 10 male dentinal tissues. But there was not detected in female pulp and dentinal tissues.
참고문헌 (28)
Erlich, HA., Gelfand, D., Sninsky, JJ. Recent advances in the polymerase chain reaction. Nature 1991;252:1643-1651
Akane, A., Seki, S., Shiono, H. et al. Sex determination of forensic samples by dual PCR amplification of an X-Y homologous gene. Forensic Science International 1992;52:143-148
Akane, A., Shiono, H., Matsubara. K. et al. Purification of forensic specimens for the polymerase chain reaction(PCR) analysis. J. of Forensic Science 1993;38(3):691-701
Goodfellw, PN. Mapping the Y chromosome. Development 1987;supplement 39
Wachtel SS. Moleculr genetics of sex determination. Academic Press Inc. 1994;2-22
Hiroki Murakami, Yuji Yamamoto et al. Forensic study of sex determinati-on using PCR on teeth samples. Acta Med Okayama 2000;54(1):21-32
Gubbay, J., Collignon, J., Koopman, PA. et al. A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes. Nature 1990;346:240-244
Harvey, W. Dental identification and forensic odontology. Henry Kimpton publishers 1976;36-37, 67-68
Hur W., Yoon CL. Comparison of 4 methods of DNA extraction for sex determination and D1S80 locus detection in teeth. Korean Journal of Oral Medicine 1995;20(2):497-512
Pfitzinger, H., Ludes, B., Mangin, P. Sex determination of forensic samples : co-amplification and sumultaneous detection of a Y-specific and an X-specific DNA sequence. International J. of Legal Medicine 1993;105: 213-216
Andrew R. Zinn, Raaji K. Alagappan et al. Structure and function of ribosomal protein S4 genes on the human and mouse sex chromosomes. Molecular and Cellular Biology 1994;2485-2492
Katsuhiko Omeoe, Akira Endo. Relationship between the monosomy X phenotype and Y-linked ribosomal protein S4(RPS4) in several species of mammals : A molecular evolutionary analysis of RPS4 homologs. Genomics 1996;31:44-50
R.L. Souhami, J. Moxham. Textbook of Medicine 1990;720
Haga M. A study of sex distinction in the teeth. Jpn. J. Legal Med. 1959;13:582-590
Vergnaud, G., Kaplan, L. et al. Rapid and early determination of sex using trophoblast biopsy specimens and Y chromosome specific DNA probes. British Medical Journal 1984;289:73-76
Witt M., Erickson RP. A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction. Hum Genet. 1989;82:271-274
David Miller. Sex determination : insights from the human and animal models suggest that the mammalian Y chromosome is uniquely specialised for the male's benefit. Jmhg. 2004;1:170-181
Ester Margarit, Anna Soler. Molecular, cytogenetic, and clinical characterisation of six XX males including one prenatal diagnosis. J. Med Genet. 1998;35:727-730
Vijay K. Dasari, Reza Z. Goharderakhshan. et al. Expression analysis of Y chromomsome genes in human prostate cancer. The Journal of Urology 2001;165:1335-1341
Yun-Fai Chris Lau, Jianqing Zhang. Expression analysis of thirty one Y chromosome genes in human prostate cancer. Molecular Carcinogenesis 2000;27:308-321
Marquis P. Vawter, Simon Evans. Gender-specific gene expression in post-mortem human brain : Localization to sex chromosomes. Neuropsychopharmacology 2004;29:373-384
Schwartz, TR., Schwartz, EA. Characterization of deoxyribonucleic acid obtained from teeth subjected to various environmental conditions. J. of Forensic Science 1991;36(4):979-990
AI-Helper
※ AI-Helper는 부적절한 답변을 할 수 있습니다.