[논문]민들레(Taraxacum officinale) 잎과 뿌리의 페놀산 조성 및 활성산소 소거활성

김영찬; 노정해; 김경탁; 조장원; 이영경; 최웅규

민들레(Taraxacum officinale) 잎과 뿌리의 페놀산 조성 및 활성산소 소거활성
Phenolic Acid Contents and ROS Scavenging Activity of Dandelion(Taraxacum officinale) 원문보기

한국식품저장유통학회지 = Korean journal of food preservation, v.15 no.3, 2008년, pp.325 - 331

김영찬 (한국식품연구원) , 노정해 (한국식품연구원) , 김경탁 (한국식품연구원) , 조장원 (한국식품연구원) , 이영경 (한국식품연구원) , 최웅규 (아시아대학교 한방식품영양학과)

Abstract ▼ AI-Helper

The propose of this study was to investigate the antioxidant activity of 80% ethanol extracts and various solvent fractions of dandelion (Taraxacmn officinale) leaves and roots, Total phenolics and phenolic acid contents were also examined. The total phenol content of leaves and roots were $7.9{\pm}0.4%$ and $9.4{\pm}0.3%$ respectively. Eight phenolic acids were separated by GC, among which caffeic acid (113.7 mg%)and m-coumaric acid (152.6 mg) were the dominant phenolic acids in leaves and roots, respectively. Amongst solvent functions of leaves and roots, the ethyl acetate fraction showed the strongest radical scavenging activity. A strong correlation was found between total phenol content and electron-donating ability, and ABTS radical scavenging activity showed a similar trend as electron-donating ability. Hydroxyl-radical-scavenging activity and lipid peroxidation were significantly higher in the ethyl acetate fraction than other factions. In particular, the SOD-like activity was highest (43.6%) in the ethyl acetate fraction of dandelion leaves, and was higher than that of trolox. Thus, the ethyl acetate fraction of dandelion leaves exhibited significant phenol content, antioxidant activity, and free-radical-scavenging effects.

주제어

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문제 정의

The aim of this study was to determine antioxidant activities of leaves and root of dandelion through a solvent fractionation against DPPH, ABTS, OH, and superoxide anion radicals. Lipid peroxidation inhibitory effect was also examined using phosphatidylcholin/ Fe2+/ascorgate system.

제안 방법

The phenolic compounds and other chemical components present in the extract may suppress lipid peroxidation through different chemical mechanism, including free radical quenching, electron transfer, radical addition or radical recombination. In this study, peroxyl radical generated through thermolysis of Fe3+ and ascorbic acid at 37℃, and efforts were made to evaluate the affinity of methanol extract and fractions of dandelion leaves and root to prevent peroxyl radical-induced damage in lipophilic model system. Ethyl acetate fraction of dandelion leaves exhibited antioxidant activity with 56.

대상 데이터

Dandelion(76troxa(?wm officinale) leaves and root were obtained from Mindulae Food Company located Kyungnam, South Korea. The leaves and root were freeze-dried and powdered.

데이터처리

‘Values within a column with different superscripts arc significantly different at p<0.05 by Duncan's multiple range test.
mean±SD(n=3). 2)Values within a column with different supeiBcripts are significantly different at ”005 by Duncan's multiple range test.
mean±SD(n=3). 2)Values within a column with different superscripts are significantly different at p<0.05 by Duncan's multiple range test.

이론/모형

DPPH radical: DPPH radical scavenging activity was determined according to the method of Blois(19). Briefly, 0.
Hydroxyl radical: Hydroxyl radical scavengin응 activity was determined by the 2-deoxyribose oxidation method(21). Hydroxyl radical was generated by the Fenton reaction in the presence of RSCM7H₂O.
Incubation was carried out for 2 hr in 37 ℃ water bath with constant shaking. Lipid peroxidation products were analyzed by the thiobarbituric method(24), their absorbance was measured at 532 nm.

후속연구

There are, however, reports of phytophenolics exhibiting antioxidant/prooxidant activities, which depend on factors like metal reducing potential, chelating behavior, pH, solubility etc. and therefore further detailed studies on fraction of dandelion and its phenolic fraction with regard to antioxidant activity in vivo are needed.

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