한국산 Red Deer (Cervus elaphus)의 심근에서 관찰된 Sarcocystis grueneri 양 포낭의 투과전자현미경 소견 Ultrastructure of Sarcocystis grueneri-like Sarcocysts from Cardiac Muscle of Red Deer (Cervus elaphus) in Korea원문보기
한국산 양축 엘크사슴 (Cervus elaphus)의 심장 조직에서 Sarcocystis grueneri 양 포낭을 광학 및 투과전자현미경으로 관찰하였다. 심장조직의 병리소견에서는 다수의 포낭이 관찰되었으며, 염증 및 근섬유의 변성은 관찰되지 않았다. 염증 및 근섬유의 변성은 관찰되지 않았으며 다수의 포낭이 관찰되었다. 포낭은 난형에서 난원형 이었으며, 크기는 90-170$\times$ 110-380 ${\mu}m$로 계측되었다. 투과전자현미경 소견에서 포낭은 숙주세포 유래의 sarcoplasm에 싸여 있었고 0.45-0.6 ${\mu}m$ 두께의 매우 얇은 포낭벽을 갖고 있었으며, 포낭벽은 protrusion 및 ground substance로 구성되어 있었다. 다수의 길고 가느다란 protrusion 층의 높이는 0.2-0.3 ${\mu}m$, protrusion의 길이는 최장 4.2 ${\mu}m$로 계측되었다. Protrusion은 포낭의 표면과 평행하게 주행하였다. 포낭은 중격에 의하여 소실로 나뉘어져 있었고 소실에는 크기가 약 $15\times4\;{\mu}m$인 다수의 merozoites가 존재하였다. 관찰된 모든 포낭은 같은 발육기를 갖고 있었다. Merozoite는 4개 구역(micronemes and rhoptries, amylopectin granules, 핵 및 amylopectin granules)으로 구분되었으며 7-11 개의 rhoptry가 충체의 전방에 분포하였다.
한국산 양축 엘크사슴 (Cervus elaphus)의 심장 조직에서 Sarcocystis grueneri 양 포낭을 광학 및 투과전자현미경으로 관찰하였다. 심장조직의 병리소견에서는 다수의 포낭이 관찰되었으며, 염증 및 근섬유의 변성은 관찰되지 않았다. 염증 및 근섬유의 변성은 관찰되지 않았으며 다수의 포낭이 관찰되었다. 포낭은 난형에서 난원형 이었으며, 크기는 90-170$\times$ 110-380 ${\mu}m$로 계측되었다. 투과전자현미경 소견에서 포낭은 숙주세포 유래의 sarcoplasm에 싸여 있었고 0.45-0.6 ${\mu}m$ 두께의 매우 얇은 포낭벽을 갖고 있었으며, 포낭벽은 protrusion 및 ground substance로 구성되어 있었다. 다수의 길고 가느다란 protrusion 층의 높이는 0.2-0.3 ${\mu}m$, protrusion의 길이는 최장 4.2 ${\mu}m$로 계측되었다. Protrusion은 포낭의 표면과 평행하게 주행하였다. 포낭은 중격에 의하여 소실로 나뉘어져 있었고 소실에는 크기가 약 $15\times4\;{\mu}m$인 다수의 merozoites가 존재하였다. 관찰된 모든 포낭은 같은 발육기를 갖고 있었다. Merozoite는 4개 구역(micronemes and rhoptries, amylopectin granules, 핵 및 amylopectin granules)으로 구분되었으며 7-11 개의 rhoptry가 충체의 전방에 분포하였다.
Sarcocystis grueneri-like sarcocysts were found from the cardiac muscles of a rearing red deer (Cervus elaphus) carcass in Korea. In the light microscopical examination of sarcocysts, they were oval to spherical cysts and 90-170$\times$110-380 ${\mu}m$ in size. However, there w...
Sarcocystis grueneri-like sarcocysts were found from the cardiac muscles of a rearing red deer (Cervus elaphus) carcass in Korea. In the light microscopical examination of sarcocysts, they were oval to spherical cysts and 90-170$\times$110-380 ${\mu}m$ in size. However, there was no inflammation and myofiber degeneration. In the transmission electron microscope, these cysts were located within the sarcoplasm of the host cell and filled with merozoites. The sarcocysts were enclosed by a very thin wall (0.45-0.6 ${\mu}m$ thick) that consists of protrusions and ground substance. The primary cyst wall formed numerous strip-like protrusions which were 0.2-0.3 ${\mu}m$ wide and up to 4.2 ${\mu}m$ long. The protrusions were running in parallel with the surface of the cyst. A characteristic of the cyst wall was absent of fibrils inside the protrusions. Merozoites in the compartment measured about $15\times4\;{\mu}m$. The merozoite consisted of four regions: micronemes and rhoptries, amylopectin granule, nucleus, and amylopectin granules. The number of rhoptry was counted in 7-13.
Sarcocystis grueneri-like sarcocysts were found from the cardiac muscles of a rearing red deer (Cervus elaphus) carcass in Korea. In the light microscopical examination of sarcocysts, they were oval to spherical cysts and 90-170$\times$110-380 ${\mu}m$ in size. However, there was no inflammation and myofiber degeneration. In the transmission electron microscope, these cysts were located within the sarcoplasm of the host cell and filled with merozoites. The sarcocysts were enclosed by a very thin wall (0.45-0.6 ${\mu}m$ thick) that consists of protrusions and ground substance. The primary cyst wall formed numerous strip-like protrusions which were 0.2-0.3 ${\mu}m$ wide and up to 4.2 ${\mu}m$ long. The protrusions were running in parallel with the surface of the cyst. A characteristic of the cyst wall was absent of fibrils inside the protrusions. Merozoites in the compartment measured about $15\times4\;{\mu}m$. The merozoite consisted of four regions: micronemes and rhoptries, amylopectin granule, nucleus, and amylopectin granules. The number of rhoptry was counted in 7-13.
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문제 정의
But the occurrence of unusual Sarcocystis species has been reported (14,17,23). This paper is the first report of S. grueneri-like sarcocysts in red deer in Korea. It will be investigated the future studies to the identification and distribution of this species of sarcocyst in the flocks in Korea.
This report described the ultrastructure of sarcocyst from red deer in Korea. The cysts found in heart tissue appear thin-walled in the light and electron microscope.
성능/효과
The final host in this study was not determined, but it is possible that these deer intermediate hosts may harbour a common species of Sarcocystis on the basis of other reports (11,18,29). In general, Sarcocystis species are specific for their intermediate hosts.
grueneri by Wesemeier and Sedaczek (31). The name was confirmed as S. grueneri. Also, they reported two more species in red deer, which were named S.
후속연구
grueneri-like sarcocysts in red deer in Korea. It will be investigated the future studies to the identification and distribution of this species of sarcocyst in the flocks in Korea.
참고문헌 (32)
Atkinson CT, Wright SD, Telford SR, Grace SJr, McLaughlin GS, Forrester DJ, Roelke ME, McCown JW. Morphology, prevalence, and distribution of Sarcocystis spp. in white-tailed deer (Odocoileus virginianus) from Florida. J Wildl Dis 1993; 29(1): 73-84
Colwell DD, Mahrt JL. Ultrastructure of the cyst wall and merozoites of Sarcocystis from moose (Alces alces) in Alberta, Canada. Z Parasitenkd 1981; 65: 317-329
Dahlgren SS, Gjerge B. Sarcocystis in moose (Alces alces): molecular identification and phylogeny of six Sarcocystis species in moose, and a morphological description of three new species. Parasitol Res 2008; 103: 93-110
Dahlgren SS, Gjerge B. Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species. Parasitol Res 2009; 104: 993-1003
Dubey JP, Speer CA, Fayer R. Sarcocystis of animals and man. CRC Press, Boca Raton, FL. 1989; 2-13
Enzeroth R. Light, scanning, and transmission electron microscope study of the cyst wall of Sarcocystis gracilis Ratz, 1909 (Sporozoa, Coccidia) from the roe deer (Capreolus capreolus L.). 1986; 129: 183-186
Enzeroth R. Chobotar B, Scholtyseck E. Ultrastructure of Sarcocystis sp. from the muscle of a white-tailed deer (Odocoileus virginianus). Z Parasitenkd 1982; 68: 33-38
Enzeroth R. Chobotar B, Scholtyseck E, Nemeseri L. Light and electron microscope study of Sarcocystis sp. from the fallow deer (Cervus dama). Z Parasitenkd 1985; 71:33-39
Entzeroth R, Nemes \acute{e} ri L, Scholtyseck E (1983) Prevalence and ultrastructure of Sarcocystis sp. from the red deer (Cervus elaphus L.) in Hungary. Parasitol Hung 16:47-52
Erber M, Boch J, Bar D. Drei Sarkosporidienarrten des Rehwides. Berl M \ddot{u} nch Tier \ddot{a} rztl Wsch 1978; 91:482-486
Gajadhar AA, Yates WDG, Allen JR. Association of eosinophilic myositis with an unusual species of Sarcocystis in a beef cow. Can J Vet Res 1987; 51: 373-378
Gjerde B. A light microscopic comparison of the cysts of four species of Sarcocystis infecting the domestic reindeer (Rangifer trandus) in northern Norway. Acta Vet Scand 1984; 25: 195-204
Gjerde B. Ultrastructure of the cysts of Sarcocystis grueneri from cardiac muscle of reindeer (Rangifer tarandus tarandus). Z Parasitenkd 1985; 71:189-198
Gjerde B. Ultrastructure of the cysts of Sarcocystis rangi from skeletal muscle of reindeer (Rangifer tarandus tarandus). Rangifer 1985; 5: 43-52
Hern \acute{a} ndez-Rodr \acute{i} guez S, Navarrete I, Mart \acute{i} nez-G \acute{o} mez F. Sarcocystis cervicanis, nueva especie parasita del ciervo (Cervus elaphus). Rev Ib \acute{e} r Parasitol 1981; 41(1): 43-51
Hudkins G, Kistner TP. Sarcocystis hemionilatrantis (Sp. N.) life cycle in mule deer and coyotes. J Wildl Dis 1977; 13: 80-84
Odening K, Quandt S, Bengis RG, Stolte M, Bockhadt I. Two new Sarcocystis species (Protozoa: Sarcocystidae) from the hippopotamus (Artiodactyla: Hippopotamidae) in South Africa. Acta Parasitol 1997; 42: 187-191
Odening K, Stolte M, Walter G, Bockhardt I. The european badger (Carnivora: Mustelidae) as intermediate host of further three Sarcocystis species (Sporozoa). Parasite 1994; 1: 23-30
Pena HFJ, Ogassawara S, Sinhorini IL. Occurrence of cattle Sarcocystis species in raw kibbe from arabian food establishments in the city of Sao Paulo, Rrazil, and experimental transmission to humans. J Parasitol 2001; 87: 1459-1465
Sedlaczek J, Wesemeier H-H. On the diagnosis and nomenclature of Sarcositis species (Sporozoa) in reo deer (Capreolus capreolus). Appl Parasitol 1995; 36: 73-82
Speer CA, Dubey JP. Sarcocystis wapiti sp. nov. from the North American wapiti (Cervus elaphus). Can J Zool 1982; 60: 881-888
Soulsby EJL. Helminths, arthropods and protozoa of domesticated animals. 7th ed. London, Balliere Tinadall 1982; 670-691
Wesemeier HH, Sedlaczek J. One known Sarcocystis species and two found for the first time in red deer and fallow deer wapiti (Cervus elaphus) in Europe. Appl Parasitol 1995; 36: 245-251
Wesemeier HH, Sedlaczek J. One known Sarcocystis species and one found for the first time in fallow deer (Dama dama). Appl Parasitol 1995; 36: 299-302
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