[논문]Characteristics of Photosynthetic Pigments during the Outbreak of Harmful Algal Bloom at the South Coastal Area in the Korean Sea Waters

Kim, Sook-Yang; Lim, Woel-Ae; Kang, Young-Sil

Characteristics of Photosynthetic Pigments during the Outbreak of Harmful Algal Bloom at the South Coastal Area in the Korean Sea Waters 원문보기

환경생물 = Korean journal of environmental biology, v.28 no.1, 2010년, pp.20 - 29

Kim, Sook-Yang (National Fisheries Research Development Institute) , Lim, Woel-Ae (National Fisheries Research Development Institute) , Kang, Young-Sil (National Fisheries Research Development Institute)

Abstract ▼ AI-Helper

Cochlodinium polykrikoides has occurs regularly during the summer in the South Sea of Korea. To investigate photosynthetic pigments concerned with phytoplankton community structure as bloom of Cochlodinium polykrikoides, the experiment was sampled at 20 stations three times between July and September 2002 in the Southern Sea of Korea. The distribution of peridinin, the biomarker of dinoflagellate, was higher at the blooming time than it was before and it disappeared after bloom. The correlative coefficients between Chl. a and peridinin at the blooming time and out of bloom were 0.9253 and 0.1613, respectively. This result indicated that the bloom was caused by dinoflagellate. The correlative coefficients between Chl. a and fucoxanthin were 0.3282 and 0.9759, respectively, and the correlative coefficients showed the succession from dinoflagellate to diatom. This result means that the bloom of Cochlodinium polykrikoides can be detected by Chl. a information from satellite remote sensing. Therefore, if the algorithm to detect peridinin in addition to Chl. a were to be developed, dinoflagellate red tide could be monitored more effectively.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

a, degradation production and other extra pigments during the red tide bloom in Korea. This study is focused on the distribution character of photosynthesis pigments before, during and after red tide bloom caused by cochlodinium polykrikoides, which has occurred annually in Korea.

대상 데이터

The photosynthesis pigments were compared and identified with a well-known pigment, which was made from Retention time and Spectrum. The standard Carbon14 (made of Centralen, DHI) was used. These photosynthesis pigments contain kinds of chlorophyll such as chl.
The study area is the central part of the south coast of Korea where harmful red tide continues to be spotted. We took samples at two different depths (surface and bottom) of 20 stations from July 20~22, August 14~16 and September 25~27 in 2002.
The study area is the central part of the south coast of Korea where harmful red tide continues to be spotted. We took samples at two different depths (surface and bottom) of 20 stations from July 20~22, August 14~16 and September 25~27 in 2002. The sea water for analyzing photosynthetic pigments and environments was collected at 0.
The pigment extraction was performed with 100% acetone as stated in UNESCO’s rule. We used four types of solvent A: Methanol (80%), Ammonium acetate (20%), 0.01% BHT, B: Acetonitrile (87.5%), H₂O (12.5%), 0.01% BHT, C: Ethyl acetate (100%), D: Methanol (100%). We injected 100 μL of an extract into HPLC, and after isolated pigments, the identification and quantification was performed by the Dual Detector (made of Waters) and the standard used Carbon14 (made of Centralen (DHI)).

데이터처리

1997) Method. The correction of results was performed with an extinction coefficient. The equation of calculation for concentration of standard pigments is as below (Park et al.
We performed a one-way ANOVA test using SPSS (ver. 10). The peridinin (a marker of dinoflagellate’s pigment) was significantly higher (p=0.

성능/효과

001) during the red tide. As shown by the results of the study about the relationship between pigments and investigated items of environment, the fucoxanthin showed a positive correlation (r²=0.404, p=0.009) with water temperature and a negative correlation (r²=0.435, p=0.004) with salinity but the chlorophyll a showed a negative correlation (r²=0.419=0.006) with salinity.
It was 11 : 27 : 1 at during red tide and 2 : 26 : 1 at the finish of red tide, so that means it was different from an ideal ratio. In addition to the Relative Enrichment factors to ideal ratio during each survey were 187 : 601 : 100, 68 : 170 : 100, 12 : 118 : 100. These means a little available used Si during the red tide than before and after of red tide.

참고문헌 (25)

Bidigare RR, JT Frank, C Zastrow and JM Brooks. 1986. The distribution of algal chlorophylls and their degradation products in thesouthern ocean. Deep-sea Research 33:923-937.

상세보기
Bjornland T and K Tangen 1979. Pigmentation and morphology of a marine Gyrodinium (Dinophyceae) with a major carotenoid different from peridinin and fucoxanthin. J. Phycol. 15:457-463.

상세보기
Foss P, T Storebakken, K Schiedt, S Liaaen-Jensen, E Austreng and K Streiff. 1984. Carotenoids in diets for salmonids.1. Pigmentation of rainbow trout with the individual optical isomers of astaxanthin in comparison with canthaxanthin. Aquaculture 41:213-226.

상세보기
Gieske WWC, GW Kraay. 1986a. Floristic and physiological difference between the shallow and the deep nanopytoplankton community in the euphotic zone of the open tropical Atlantic reveales by HPLC analysis of pigments. Marine Biology 91:567-576.

상세보기
Gieskes WWC and GW Kraay. 1983. Dominance of Cryptophyceae during the phytoplankton spring bloom in the central North Sea detected by HPLC analysis of pigments. Marine Biology 75:179-185.

상세보기
Gieskes WWC and GW Kraay. 1986b. Analysis of phytoplankton by HPLc before, during and after mass occurrence of the microflagellate Corymbellus during the spring bloom in the open northern North Sea in 1983. Marine Biology 92:45-52.

상세보기
Gieskes WWC and GW Kraay. 1988. Monsoonal alternation of a mixed and a layered structure in the phytoplankton of the euphotic zone of the Banda Sea (indonesia): A mathematical analysis of algal pigment fingerprints. NETH. J. Sea Res. 22:123-137.

상세보기
Kim HG, CS Jung, WA Lim, CK Lee, SY Kim, SH Youn, YC Cho and SG Lee. 2001. The Spatio-Temporal Progress of Cochlodinium polykrikoides blooms in the coastal waters of Korea. J. Korean Fish. Soc. 34:691-696.

원문보기 상세보기
Hooks CE, RR Bidigare, MD Keller and RRL Guillard. 1988. Coccoid eukaryotic marine ultraplankters with four different HPLC pigment signatures. J. Phycol. 24:571-580.

상세보기
Hurley JP and CJ Watras. 1991. Identification of bacteriochlorophylls in lakes via reverse-phase HPLC. Limnology and Oceanography. 32:307315.
Jeffery SW. 1974. Profile of photosynthetic pigments in the ocean using thin layer chromatography. Mar. Biol. 26:101-110.

상세보기
Karthals HJ and CLM Steenbergen. 1985. Seperation and quantification of pigments from natural phototrophic microbial populations. FED. Microbial. Ecol. 31:177-185.

상세보기
Lorenzen CJ. 1967. Determination of chlorophyll and phaeopigments: spectrophotometric equations, Limnol. Oceanogr. 12.
Lorenzen CJ. 1974. Chlorophyll-degradation products sediments of Black sea -geology, chemistry, and biology. E. Degens and D.A.Ross, editors, Memoris of the American Association of Petroleum Geologists. 20:426-428.
Mantoura RFC and CA Llewllyn. 1983. The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by Reverse-Phase High-Performance Liquid Chromatography. Analytica Chimica Acta. 151:297-314.

상세보기
Nelson JR. 1989. Phytoplankton pigments in macrozooplankton feces: Variability in carotenoid alterations. Mar. Ecol. Prog. Ser. 52:129-144.

상세보기
Roy S. 1988. Effects of changes in Physiological condition on HPLC-defined chloropigment composition of Phaeodactylum tricornutum (Bohlin) in batch and turbidostat cultures. J. Exp. Mar. Ecol. 18:137-149.
Wright SW, SW Jeffrey, RFC Mantoura, CA Llewellyn, T Bjornland, D Repeta and N Welschmeyer. 1991. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Mar. Ecol. Prog. Ser. 77:183-196.

상세보기
Sun S, FX Cunningham and E Gantt. 1998. Differential expression of two isopentenyl pyrophosphate isomerases and enhanced carotenoid accumulation in a unicellular chlorophyte. Proc. Natl. Acad. Sci. USA. 95:11482-11488.

상세보기
WA Lim, CK Kang, SY Kim, SG Lee, HG Kim and IK Chung. 2003. Short-term Changes of Community srtucture of Phytoplankton in summer around Namhae Island of Korea. J. Korean Algae. Soc. 18:49-58.
Wong GTF, GC Gong, KK Liu and SC Pai. 1998. Excess Nitrate in the East China Sea, Esturine, Coastal and Shelf Science 46:411-418.

상세보기
Wright SW and JD Shearer. 1984. Rapid extraction and high performance liquid chromatography of chlorophylls and carotenoids from marine phytoplankton. J. Chromatogr. 294:281-295.

상세보기
Wright SW and SW Jeffery. 1987. Fucoxanthin pigment markers of marine phytoplankton analysrd by HPLC and HPTLC. Mar. Ecol (Prog. Ser.). 38:259-266.

상세보기
Yacobi YZ, RFC Mantoura and CA Llewellyn. 1991. The distribution of chlorophylls, carotenoids and their breakdown products in Lake Kinneret (Israel) sediments. Freshwater biology. 26:1-10.

상세보기
Zapata M, AM Ayaia, JM Franco and JL Garrido. 1987. Separation of chlorophylls and their degradation products in marine phytoplankton by reverse high-performance liquid chromatography. Chromatographia 23:26-30.

상세보기

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증