[논문]RAPD분자 마커를 이용한 왕대속 대나무의 유전적 다양성 및 계통 관계

이송진; 허만규; 신현철; 허홍욱

doi:10.5352/jls.2010.20.6.819

RAPD분자 마커를 이용한 왕대속 대나무의 유전적 다양성 및 계통 관계
Genetic Diversity and Phylogenetic Relationship of Genus Phyllostachys by RAPD Markers 원문보기

생명과학회지 = Journal of life science, v.20 no.6 = no.122, 2010년, pp.819 - 824

이송진 (부산대학교 생물학과) , 허만규 (동의대학교 분자생물학과) , 신현철 (국립산림과학원 남부산림연구원) , 허홍욱 (부산대학교 생물학과)

초록
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왕대속 대나무들은 대부분 동남아시아에 분포한다. 전세계적으로 왕대속에 속하는 4종은 의학적, 생태학적으로 중요시 되어 왔다. 이번 연구에서 우리나라에 자생하고 있는 왕대속 4종을 RAPD마커를 이용하여 유전적 관계 분석하였다. RAPD분석결과 왕대속에 속하는 4종의 대나무는 명확하게 분류가 되었고 8.9~33.3%로 다형현상이 나타났다. 특히 왕대는 다른 종들 보다 유전적 다양성이 0.018로 가장 낮게 나왔다. 그리고 집단 내 유전적 다양성(Hs)은 0.315, 집단간 다양성(Gst)은 0.659 그리고 유전자 유동(Nm)은 0.0263로 나타났다. 이는 한국의 왕대속 집단은 지리적 및 환경적 요인을 받아 유전적 다양성이 낮게 나타났으며 본 연구는 대나무 유전적 다양성 연구에 중요한 기초자료가 될 것으로 사료된다.

Abstract ▼ AI-Helper

Genus Phyllostachys is a long-lived woody species primarily distributed throughout South East Asia. Many species of this genus has been regarded as medically and ecologically important in the world. We evaluated representative samples of the four taxa with RAPD to estimate genetic relationships within the genus Phyllostachys. The percentages of polymorphic loci were 8.9-33.3% at the species level. P. bambusoides was found to show lower genetic diversity (H=0.018) than other species. Total genetic diversity ($H_T$) was 0.315, genetic diversity within populations ($H_S$) was 0.043, the proportion of total genetic diversity partitioned among populations ($G_{ST}$) was 0.659 and the gene flow (Nm) was 0.0263. As some Korean populations were isolated and patchily distributed, they exhibited low levels of genetic diversity. The four taxa of the genus Phyllostachys analyzed were distinctly related to a monophyletic. P. nigra var. henonis. Stapf was found to be more closely related to P. pubescens than to P. nigra. P. bambusoides was quite distinct from the remaining species.

주제어

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제안 방법

In this study, RAPDs were used to determine the genetic relationships among 12 populations and the results were compared to pedigree relationships where they were available.
All the reactions were repeated twice and only reproducible bands were scored for analyses. To analyze the DNA of individuals, we selected nine decamer primers that produced RAPD bands in four species in a preliminary test (Table 2).

이론/모형

A phylogenetic tree was constructed by the neighbor-joining (NJ) method [19] using the NEIGHBOR program in PHYLIP version 3.57 [5].
Clustering of three populations per species, using the NJ algorithm, was performed based on the matrix of calculated distances (Fig. 1). The four taxa of genus Phyllostachys analyzed were distinctly related to a monophyletic.
The degree of polymorphism was quantified using Shannon’s diversity index (I) [20].

성능/효과

1). In the phylogeny generated, the four taxa of the genus Phyllostachys analyzed were distinctly related to a monophyletic. Since species of Phyllostachys show a wide range of morphological and geographical variation, it is difficult to elucidate phylogenetic relationships with morphological characteristics [10].
1). The four taxa of genus Phyllostachys analyzed were distinctly related to a monophyletic. P.

후속연구

nigra, and thus deserve taxonomic treatment at subspecies or even at species level. Although the size of sampling was not large enough for the genus Phyllostachys, the analyses of RAPDs will certainly provide an enhanced view on the phylogeny of this genus. In addition, It is necessary to identify taxa that additional molecular experiments such as AFLP, SSR, and ITS

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